PMID- 31055804
OWN - NLM
STAT- MEDLINE
DCOM- 20190813
LR  - 20211204
IS  - 1003-9406 (Print)
IS  - 1003-9406 (Linking)
VI  - 36
IP  - 6
DP  - 2019 Jun 10
TI  - [Characterizing the molecular cytogenetics in acute monocytic leukemia].
PG  - 556-560
LID - 10.3760/cma.j.issn.1003-9406.2019.06.006 [doi]
AB  - OBJECTIVE: To characterize the molecular genetics of 81 patients with acute 
      monocytic leukemia (AML). METHODS: Fluorescence in situ hybridization (FISH) was 
      employed to detect MLL gene rearrangements. Combined mutations of 17 genes were 
      detected by DNA-based PCR and Sanger sequencing. RESULTS: Sixty seven patients 
      were found to harbor at least one mutation. The most commonly mutated gene was 
      NPM1 (n=18), which was followed by FLT3-ITD (n=16), NRAS (n=16), DNMT3A (n=15), 
      TET2 (n=12), RUNX1 (n=11) and KRAS (n=9). Based on the functions of mutated 
      genes, the most frequently involved genes were those involved in DNA methylation 
      (38.27%), tyrosine kinase receptor signaling (32.1%), transcription regulation 
      (28.4%), and RAS pathway (24.7%). Single gene mutation predominated in patient 
      with cytogenetic abnormalities, while coexistence of 2 mutations have 
      predominated in patient with normal cytogenetic findings. Stratified by 
      cytogenetic findings, patients with single gene mutations (intermediate-risk 
      group) had significantly higher complete remission (CR) rates than those with >/=2 
      gene mutations (unfavorable-risk group) (91.7% vs. 57.6% , 87.5% vs. 25.0%, P 
      =0.0319, 0.0117, respectively). CONCLUSION: Over 80% of AML patients were found 
      to harbor at least one mutation. Their clinical phenotype and prognosis may be 
      impacted by the synergy of MLL gene rearrangement and multiple mutations. For 
      patients under the same risk stratification, the number of mutations is reversely 
      correlated with the CR rate.
FAU - Zhou, Feng
AU  - Zhou F
AD  - Department of Hematology, Changzhou Second People's Hospital Affiliated to 
      Nanjing Medical University, Changzhou, Jiangsu 213003, China. Email: 
      chaohy2006@126.com.
FAU - Chao, Hongying
AU  - Chao H
FAU - Lu, Xuzhang
AU  - Lu X
FAU - Chen, Tao
AU  - Chen T
FAU - Yang, Jianhe
AU  - Yang J
FAU - Jiang, Naike
AU  - Jiang N
FAU - Cen, Ling
AU  - Cen L
FAU - Zhou, Min
AU  - Zhou M
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi
JT  - Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese 
      journal of medical genetics
JID - 9425197
RN  - 0 (NPM1 protein, human)
RN  - 117896-08-9 (Nucleophosmin)
RN  - EC 2.7.10.1 (fms-Like Tyrosine Kinase 3)
MH  - Cytogenetics
MH  - Humans
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Monocytic, Acute
MH  - *Leukemia, Myeloid, Acute
MH  - Mutation
MH  - Nucleophosmin
MH  - Prognosis
MH  - fms-Like Tyrosine Kinase 3
EDAT- 2019/05/06 06:00
MHDA- 2019/08/14 06:00
CRDT- 2019/05/06 06:00
PHST- 2019/05/06 06:00 [entrez]
PHST- 2019/05/06 06:00 [pubmed]
PHST- 2019/08/14 06:00 [medline]
AID - 940636109 [pii]
AID - 10.3760/cma.j.issn.1003-9406.2019.06.006 [doi]
PST - ppublish
SO  - Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2019 Jun 10;36(6):556-560. doi: 
      10.3760/cma.j.issn.1003-9406.2019.06.006.