PMID- 31058887
OWN - NLM
STAT- MEDLINE
DCOM- 20200324
LR  - 20200324
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 146
DP  - 2019 Apr 18
TI  - Generation of 3D Skin Organoid from Cord Blood-derived Induced Pluripotent Stem 
      Cells.
LID - 10.3791/59297 [doi]
AB  - The skin is the body's largest organ and has many functions. The skin acts as a 
      physical barrier and protector of the body and regulates bodily functions. 
      Biomimetics is the imitation of the models, systems, and elements of nature for 
      the purpose of solving complex human problems(1). Skin biomimetics is a useful 
      tool for in vitro disease research and in vivo regenerative medicine. Human 
      induced pluripotent stem cells (iPSCs) have the characteristic of unlimited 
      proliferation and the ability of differentiation to three germ layers. Human 
      iPSCs are generated from various primary cells, such as blood cells, 
      keratinocytes, fibroblasts, and more. Among them, cord blood mononuclear cells 
      (CBMCs) have emerged as an alternative cell source from the perspective of 
      allogeneic regenerative medicine. CBMCs are useful in regenerative medicine 
      because human leukocyte antigen (HLA) typing is essential to the cell banking 
      system. We provide a method for the differentiation of CBMC-iPSCs into 
      keratinocytes and fibroblasts and for generation of a 3D skin organoid. 
      CBMC-iPSC-derived keratinocytes and fibroblasts have characteristics similar to a 
      primary cell line. The 3D skin organoids are generated by overlaying an epidermal 
      layer onto a dermal layer. By transplanting this 3D skin organoid, a humanized 
      mice model is generated. This study shows that a 3D human iPSC-derived skin 
      organoid may be a novel, alternative tool for dermatologic research in vitro and 
      in vivo.
FAU - Kim, Yena
AU  - Kim Y
AD  - CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, 
      College of Medicine, The Catholic University of Korea; Department of Biomedicine 
      & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic 
      University of Korea.
FAU - Ju, Ji Hyeon
AU  - Ju JH
AD  - CiSTEM Laboratory, Catholic Induced Pluripotent Stem Cell (iPSC) Research Center, 
      College of Medicine, The Catholic University of Korea; Department of Biomedicine 
      & Health Science, Seoul St. Mary's Hospital, College of Medicine, The Catholic 
      University of Korea; Division of Rheumatology, Department of Internal Medicine, 
      Seoul St. Mary's Hospital, College of Medicine, The Catholic University of Korea; 
      juji@catholic.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20190418
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
SB  - IM
MH  - Animals
MH  - *Cell Differentiation
MH  - Cell Line
MH  - Fetal Blood/cytology
MH  - Fibroblasts/*cytology
MH  - Humans
MH  - Induced Pluripotent Stem Cells/*cytology
MH  - Keratinocytes/*cytology
MH  - Mice
MH  - Organoids/*growth & development
MH  - Regenerative Medicine
MH  - Skin/cytology/*growth & development
EDAT- 2019/05/07 06:00
MHDA- 2020/03/25 06:00
CRDT- 2019/05/07 06:00
PHST- 2019/05/07 06:00 [entrez]
PHST- 2019/05/07 06:00 [pubmed]
PHST- 2020/03/25 06:00 [medline]
AID - 10.3791/59297 [doi]
PST - epublish
SO  - J Vis Exp. 2019 Apr 18;(146). doi: 10.3791/59297.