PMID- 31058899
OWN - NLM
STAT- MEDLINE
DCOM- 20200325
LR  - 20200325
IS  - 1940-087X (Electronic)
IS  - 1940-087X (Linking)
IP  - 146
DP  - 2019 Apr 21
TI  - Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific 
      Immunoglobulin E (sIgE).
LID - 10.3791/59100 [doi]
AB  - Allergic disease is common in both adults and children. Identification of the 
      causative allergens is significant in disease management and prevention. However, 
      a specific immunoglobulin E (IgE) measurement system with a high 
      price-performance ratio is lacking in mainland China, especially in the primary 
      care hospitals. This paper describes the principle and operation procedures of 
      using a microfluidic cartridge-based chemiluminescence system to detect 
      allergen-specific IgE in serum. The results were compared with those from 
      ImmunoCAP (System 1), the industrial standard, and the reproducibility of the 
      system to detect patients sensitized to common allergens is evaluated. The 
      results showed that in comparison with ImmunoCAP (System 1), the BioIC System 
      (System 2) has good precision and sensitivity in detecting serum-specific IgE 
      against various inhalant and food allergens but with a significantly lower cost. 
      It can serve as a good alternative to System 1 in primary care hospitals in 
      mainland China who have lower financial affordability.
FAU - Huang, Zhifeng
AU  - Huang Z
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Luo, Wenting
AU  - Luo W
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Zou, Xiangwei
AU  - Zou X
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Liu, Xiaoqing
AU  - Liu X
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Cai, Chuanxu
AU  - Cai C
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Wu, Zehong
AU  - Wu Z
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Hu, Haisheng
AU  - Hu H
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University.
FAU - Sun, Baoqing
AU  - Sun B
AD  - Guangzhou Institute of Respiratory Health, State Key Laboratory of Respiratory 
      Disease, First Affiliated Hospital of Guangzhou Medical University, Guangzhou 
      Medical University; sunbaoqing@vip.163.com.
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Video-Audio Media
DEP - 20190421
PL  - United States
TA  - J Vis Exp
JT  - Journal of visualized experiments : JoVE
JID - 101313252
RN  - 0 (Allergens)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Allergens/analysis/*immunology
MH  - Antibody Specificity
MH  - Child
MH  - China
MH  - Female
MH  - Food
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Male
MH  - Microarray Analysis/*methods
MH  - *Microfluidics
MH  - Reproducibility of Results
MH  - Young Adult
EDAT- 2019/05/07 06:00
MHDA- 2020/03/26 06:00
CRDT- 2019/05/07 06:00
PHST- 2019/05/07 06:00 [entrez]
PHST- 2019/05/07 06:00 [pubmed]
PHST- 2020/03/26 06:00 [medline]
AID - 10.3791/59100 [doi]
PST - epublish
SO  - J Vis Exp. 2019 Apr 21;(146). doi: 10.3791/59100.