PMID- 31060342
OWN - NLM
STAT- MEDLINE
DCOM- 20190821
LR  - 20200225
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 20
IP  - 9
DP  - 2019 May 5
TI  - Molecular Signature of Prospero Homeobox 1 (PROX1) in Follicular Thyroid 
      Carcinoma Cells.
LID - 10.3390/ijms20092212 [doi]
LID - 2212
AB  - The prospero homeobox 1 (PROX1) transcription factor is a product of one of the 
      lymphangiogenesis master genes. It has also been suggested to play a role in 
      carcinogenesis, although its precise role in tumour development and metastasis 
      remains unclear. The aim of this study was to gain more knowledge on the PROX1 
      function in thyroid tumorigenesis. Follicular thyroid cancer-derived 
      cells-CGTH-W-1-were transfected with PROX1-siRNA (small interfering RNA) and 
      their proliferation, cell cycle, apoptosis and motility were then analysed. The 
      transcriptional signature of PROX1 depletion was determined using RNA-Sequencing 
      (RNA-Seq) and the expression of relevant genes was further validated using 
      reverse transcriptase quantitative PCR (RT-qPCR), Western blot and 
      immunocytochemistry. PROX1 depletion resulted in a decreased cell motility, with 
      both migratory and invasive potential being significantly reduced. The cell 
      morphology was also affected, while the other studied cancer-related cell 
      characteristics were not significantly altered. RNA-seq analysis revealed 
      significant changes in the expression of transcripts encoding genes involved in 
      both motility and cytoskeleton organization. Our transcriptional analysis of 
      PROX1-depleted follicular thyroid carcinoma cells followed by functional and 
      phenotypical analyses provide, for the first time, evidence that PROX1 plays an 
      important role in the metastasis of thyroid cancer cells by regulating genes 
      involved in focal adhesion and cytoskeleton organization in tumour cells.
FAU - Rudzinska, Magdalena
AU  - Rudzinska M
AD  - Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical 
      Education, 01-813 Warsaw, Poland. magdda.rudzinska@gmail.com.
FAU - Grzanka, Malgorzata
AU  - Grzanka M
AD  - Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical 
      Education, 01-813 Warsaw, Poland. mgrzanka@cmkp.edu.pl.
FAU - Stachurska, Anna
AU  - Stachurska A
AD  - Department of Immunohematology, Centre of Postgraduate Medical Education, 01-813 
      Warsaw, Poland. astachurska@cmkp.edu.pl.
FAU - Mikula, Michal
AU  - Mikula M
AD  - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and 
      Institute of Oncology, 02-781 Warsaw, Poland. mikula.michal@gmail.com.
FAU - Paczkowska, Katarzyna
AU  - Paczkowska K
AD  - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and 
      Institute of Oncology, 02-781 Warsaw, Poland. paczkowska.km@gmail.com.
FAU - Stepien, Tomasz
AU  - Stepien T
AD  - Clinic of Endocrinological and General Surgery, Medical University of Lodz, 
      93-513 Lodz, Poland. tomsamaz@wp.pl.
FAU - Paziewska, Agnieszka
AU  - Paziewska A
AD  - Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of 
      Postgraduate Medical Education, 01-813 Warsaw, Poland. apaziewska@cmkp.edu.pl.
FAU - Ostrowski, Jerzy
AU  - Ostrowski J
AD  - Department of Genetics, Maria Sklodowska-Curie Memorial Cancer Center and 
      Institute of Oncology, 02-781 Warsaw, Poland. jostrow@warman.com.pl.
AD  - Department of Gastroenterology, Hepatology and Clinical Oncology, Centre of 
      Postgraduate Medical Education, 01-813 Warsaw, Poland. jostrow@warman.com.pl.
FAU - Czarnocka, Barbara
AU  - Czarnocka B
AD  - Department of Biochemistry and Molecular Biology, Centre of Postgraduate Medical 
      Education, 01-813 Warsaw, Poland. bczarnocka@cmkp.edu.pl.
LA  - eng
GR  - 2013/11/N/NZ5/03394 (to M.R.) and 2012/07/B/ NZ5/02444 (to B.C.)/The National 
      Science Center, Poland/
PT  - Journal Article
DEP - 20190505
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (Tumor Suppressor Proteins)
RN  - 0 (prospero-related homeobox 1 protein)
MH  - Adenocarcinoma, Follicular/*genetics/pathology
MH  - Apoptosis/genetics
MH  - Biomarkers, Tumor
MH  - Biopsy
MH  - Cell Cycle/genetics
MH  - Cell Line, Tumor
MH  - Cell Movement
MH  - Cell Survival/genetics
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - High-Throughput Nucleotide Sequencing
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - Immunohistochemistry
MH  - RNA, Small Interfering/genetics
MH  - Transcriptome
MH  - Tumor Suppressor Proteins/*genetics
PMC - PMC6539481
OTO - NOTNLM
OT  - PROX1
OT  - RNA-seq
OT  - cytoskeleton
OT  - follicular thyroid carcinoma
OT  - invasion
OT  - migration
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses or interpretation of data; in 
      the writing of the manuscript or in the decision to publish the results.
EDAT- 2019/05/08 06:00
MHDA- 2019/08/23 06:00
PMCR- 2019/05/01
CRDT- 2019/05/08 06:00
PHST- 2019/03/13 00:00 [received]
PHST- 2019/04/24 00:00 [revised]
PHST- 2019/04/28 00:00 [accepted]
PHST- 2019/05/08 06:00 [entrez]
PHST- 2019/05/08 06:00 [pubmed]
PHST- 2019/08/23 06:00 [medline]
PHST- 2019/05/01 00:00 [pmc-release]
AID - ijms20092212 [pii]
AID - ijms-20-02212 [pii]
AID - 10.3390/ijms20092212 [doi]
PST - epublish
SO  - Int J Mol Sci. 2019 May 5;20(9):2212. doi: 10.3390/ijms20092212.