PMID- 31061298
OWN - NLM
STAT- MEDLINE
DCOM- 20190806
LR  - 20190806
IS  - 1347-5223 (Electronic)
IS  - 0009-2363 (Linking)
VI  - 67
IP  - 8
DP  - 2019 Aug 1
TI  - A Probe for Fluorescence Detection of the Acetylcholinesterase Activity Based on 
      Molecularly Imprinted Polymers Coated Carbon Dots.
PG  - 795-800
LID - 10.1248/cpb.c18-00944 [doi]
AB  - This paper presents a new probe for fluorescence detection of the 
      acetylcholinesterase (AChE) activity based on molecularly imprinted polymer (MIP) 
      coated carbon dots (C-dots) composite. The C-dots were hydrothermally synthesized 
      with grafted silica surface and sealed with molecularly imprinted polymers in 
      silica pores (MIP@C-dots) in situ. Removed the original template molecules, the 
      MIP@C-dots composite exhibits quite high selectivity for acetylthiocholine (ACh). 
      With AChE, its substrate ACh will be hydrolyzed into thiocholine and the 
      fluorescence signals exhibit a dramatic decrease at 465 nm, Under optimal 
      conditions, the fluorescent probe shows sensitive responses to AChE in the range 
      of 0.01-0.6 mU/mL. The detection limits of AChE are as low as 3 microU/mL. These 
      experiments results validate the novel fluorescent probe based on MIP@C-dots 
      composite, paving a new way to evaluation of AChE activity and Screening 
      inhibitors.
FAU - Jia, Zhao
AU  - Jia Z
AD  - School of Chemical Engineering, Northwest University.
AD  - Shangluo University.
FAU - Luo, Yane
AU  - Luo Y
AD  - College of Food Science and Technology, Northwest University.
FAU - Wen, Huiyun
AU  - Wen H
AD  - School of Chemical Engineering, Northwest University.
FAU - Huang, Saipeng
AU  - Huang S
AD  - School of Chemical Engineering, Northwest University.
FAU - Du, Xin
AU  - Du X
AD  - School of Chemical Engineering, Northwest University.
FAU - Xue, Weiming
AU  - Xue W
AD  - School of Chemical Engineering, Northwest University.
LA  - eng
PT  - Journal Article
DEP - 20190426
PL  - Japan
TA  - Chem Pharm Bull (Tokyo)
JT  - Chemical & pharmaceutical bulletin
JID - 0377775
RN  - 0 (Cholinesterase Inhibitors)
RN  - 0 (Fluorescent Dyes)
RN  - 0 (Polymers)
RN  - 7440-44-0 (Carbon)
RN  - 7631-86-9 (Silicon Dioxide)
RN  - EC 3.1.1.7 (Acetylcholinesterase)
SB  - IM
MH  - Acetylcholinesterase/*analysis/metabolism
MH  - Carbon/*chemistry
MH  - Cholinesterase Inhibitors/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Fluorescence
MH  - Fluorescent Dyes/*chemistry
MH  - Humans
MH  - Molecular Conformation
MH  - *Molecular Imprinting
MH  - Polymers/*chemistry
MH  - Quantum Dots/*chemistry
MH  - Silicon Dioxide/chemistry
MH  - Structure-Activity Relationship
OTO - NOTNLM
OT  - acetylcholinesterase
OT  - carbon dot
OT  - fluorescence probe
OT  - surface imprinting
EDAT- 2019/05/08 06:00
MHDA- 2019/08/07 06:00
CRDT- 2019/05/08 06:00
PHST- 2019/05/08 06:00 [pubmed]
PHST- 2019/08/07 06:00 [medline]
PHST- 2019/05/08 06:00 [entrez]
AID - 10.1248/cpb.c18-00944 [doi]
PST - ppublish
SO  - Chem Pharm Bull (Tokyo). 2019 Aug 1;67(8):795-800. doi: 10.1248/cpb.c18-00944. 
      Epub 2019 Apr 26.