PMID- 3106158
OWN - NLM
STAT- MEDLINE
DCOM- 19870605
LR  - 20190707
IS  - 0378-1119 (Print)
IS  - 0378-1119 (Linking)
VI  - 49
IP  - 2
DP  - 1986
TI  - The beta-glucanase gene from Bacillus amyloliquefaciens shows extensive homology 
      with that of Bacillus subtilis.
PG  - 177-87
AB  - The nucleotide sequence of a 1583-bp DNA fragment containing gene bg1A for 
      endo-beta-1,3-1,4-glucanase (EC 3.2.1.73) of Bacillus amyloliquefaciens strain 
      BE20/78, a high producer of secreted enzymes, has been determined. The gene bg1A 
      comprises an open reading frame (ORF) of 717 bp (= 239 codons) starting with ATG 
      at 469 up to the translation stop codon TAA at 1188. Upstream from the 
      translation initiation codon ATG, the ribosome-binding sequence 5'-AAAAAAGGGGG-3' 
      and two putative bglA promoters have been identified. A box of eleven AT out of 
      twelve base pairs (bp) precedes the -35 region of promoter P1. Beyond the 
      translation stop codon UAA, a sequence of 69 bp can be folded into a hook-like 
      stem-loop structure which probably functions as a transcriptional terminator. The 
      ORF region of the gene bglA reveals about 90% homology with another 
      beta-glucanase gene, bglS of Bacillus subtilis C120 sequenced by Murphy et al. 
      (1984). Three regions of frequent amino acid (aa) changes are indicated. However, 
      the major difference between these is a set of deletions within the non-coding 
      region separating the bglA gene from an unknown preceding ORF and by one deletion 
      shortening the proposed signal peptide by three aa (Pro-Tyr-Leu-). The putative 
      transcription terminator of gene bglA completely lacks homology with a B. 
      subtilis bglS gene. The signification of deletions erasing the 'sacR-homology 
      region' in B. amyloliquefaciens, which have been detected in proximity of the 
      beta-glucanase gene of B. subtilis by Steinmetz and Aymerich (1986), is 
      discussed.
FAU - Hofemeister, J
AU  - Hofemeister J
FAU - Kurtz, A
AU  - Kurtz A
FAU - Borriss, R
AU  - Borriss R
FAU - Knowles, J
AU  - Knowles J
LA  - eng
SI  - GENBANK/M15674
PT  - Comparative Study
PT  - Journal Article
PL  - Netherlands
TA  - Gene
JT  - Gene
JID - 7706761
RN  - 0 (Bacterial Proteins)
RN  - 0 (DNA, Bacterial)
RN  - 0 (DNA, Recombinant)
RN  - EC 3.2.1.- (Glycoside Hydrolases)
RN  - EC 3.2.1.73 (licheninase)
SB  - IM
MH  - Bacillus/enzymology/*genetics
MH  - Bacillus subtilis/enzymology/genetics
MH  - Bacterial Proteins/*genetics
MH  - DNA, Bacterial/genetics
MH  - DNA, Recombinant/analysis
MH  - Genes
MH  - *Genes, Bacterial
MH  - Genes, Regulator
MH  - Glycoside Hydrolases/*genetics
MH  - Sequence Homology, Nucleic Acid
MH  - Species Specificity
MH  - Transcription, Genetic
EDAT- 1986/01/01 00:00
MHDA- 1986/01/01 00:01
CRDT- 1986/01/01 00:00
PHST- 1986/01/01 00:00 [pubmed]
PHST- 1986/01/01 00:01 [medline]
PHST- 1986/01/01 00:00 [entrez]
AID - 0378-1119(86)90278-7 [pii]
AID - 10.1016/0378-1119(86)90278-7 [doi]
PST - ppublish
SO  - Gene. 1986;49(2):177-87. doi: 10.1016/0378-1119(86)90278-7.