PMID- 31062434
OWN - NLM
STAT- MEDLINE
DCOM- 20191111
LR  - 20191111
IS  - 1939-165X (Electronic)
IS  - 0275-6382 (Linking)
VI  - 48
IP  - 2
DP  - 2019 Jun
TI  - Hematologic, biochemical, hormonal, and redox status alterations during 
      reproductive activity in adult rams of two breeds.
PG  - 347-354
LID - 10.1111/vcp.12731 [doi]
AB  - BACKGROUND: Reproduction is a crucial, energy-consuming physiologic activity, 
      which can temporarily disturb the homeostatic balance of the organism. Although 
      rams are commonly placed in intense breeding programs on contemporary sheep 
      farms, the clinicopathologic and redox status alterations have not been 
      adequately studied during reproductive activity. OBJECTIVES: The objective was to 
      investigate the clinicopathologic and redox status changes in rams during 
      reproductive activity. METHODS: Thirty-seven (19 Chios and 18 Florina breed) 
      adult, healthy rams were individually mated with ten estrus-synchronized ewes of 
      the same breed. Blood samples were collected prior to mating, during mating, and 
      postmating. CBC (Advia 120), cytologic blood smear evaluation, biochemical 
      analyses (Flexor E, AVL 9180 Electrolyte Analyzer), and thyroxine measurements 
      (Immulite 1000) were performed. The reactive oxygen metabolites (d-ROMs) test, 
      the biological antioxidant potential (BAP) test, and oxidative stress index were 
      used to assess redox status. The linear mixed effects model (statistical language 
      R) was used for statistical analyses. RESULTS: Many, mostly breed-independent, 
      clinicopathologic changes were detected during reproductive activity. Conversely, 
      redox status alterations were found to be breed-dependent. CONCLUSIONS: Intense 
      reproductive activity was related to mild, acute muscle damage in both breeds and 
      mild oxidative stress in Florina rams, whereas Chios rams exhibited oxidative 
      stress resistance. The observed clinicopathologic and redox status alterations 
      were generally consistent with those reported during various forms of physical 
      activity and exercise in animals and humans. Intense reproductive activity does 
      not seem to be physiologically innocuous; however, it appears to be a relatively 
      mild muscle-damaging physical activity.
CI  - (c) 2019 American Society for Veterinary Clinical Pathology.
FAU - Oikonomidis, Ioannis L
AU  - Oikonomidis IL
AUID- ORCID: 0000-0002-1591-9891
AD  - Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, 
      Aristotle University of Thessaloniki, Thessaloniki, Greece.
FAU - Brozos, Christos
AU  - Brozos C
AD  - Farm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, 
      Aristotle University of Thessaloniki, Thessaloniki, Greece.
FAU - Kiossis, Evangelos
AU  - Kiossis E
AD  - Farm Animal Clinic, School of Veterinary Medicine, Faculty of Health Sciences, 
      Aristotle University of Thessaloniki, Thessaloniki, Greece.
FAU - Kritsepi-Konstantinou, Maria
AU  - Kritsepi-Konstantinou M
AD  - Diagnostic Laboratory, School of Veterinary Medicine, Faculty of Health Sciences, 
      Aristotle University of Thessaloniki, Thessaloniki, Greece.
LA  - eng
PT  - Journal Article
DEP - 20190506
PL  - United States
TA  - Vet Clin Pathol
JT  - Veterinary clinical pathology
JID - 9880575
RN  - 0 (Antioxidants)
RN  - Q51BO43MG4 (Thyroxine)
SB  - IM
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Blood Cell Count/veterinary
MH  - Breeding
MH  - Male
MH  - Oxidation-Reduction
MH  - Oxidative Stress
MH  - *Reproduction
MH  - Sheep/blood/*physiology
MH  - Species Specificity
MH  - Thyroxine/blood
OTO - NOTNLM
OT  - biochemistry
OT  - breeding
OT  - complete blood count
OT  - male sheep
OT  - mating
OT  - oxidative stress
EDAT- 2019/05/08 06:00
MHDA- 2019/11/12 06:00
CRDT- 2019/05/08 06:00
PHST- 2018/02/12 00:00 [received]
PHST- 2018/08/31 00:00 [revised]
PHST- 2018/12/05 00:00 [accepted]
PHST- 2019/05/08 06:00 [pubmed]
PHST- 2019/11/12 06:00 [medline]
PHST- 2019/05/08 06:00 [entrez]
AID - 10.1111/vcp.12731 [doi]
PST - ppublish
SO  - Vet Clin Pathol. 2019 Jun;48(2):347-354. doi: 10.1111/vcp.12731. Epub 2019 May 6.