PMID- 31062928
OWN - NLM
STAT- MEDLINE
DCOM- 20200619
LR  - 20200619
IS  - 1932-7005 (Electronic)
IS  - 1932-6254 (Linking)
VI  - 13
IP  - 8
DP  - 2019 Aug
TI  - The cytoptrotection of small intestinal submucosa-derived gel in HL-1 cells 
      during hypoxia/reoxygenation-induced injury.
PG  - 1346-1361
LID - 10.1002/term.2878 [doi]
AB  - Small intestinal submucosa (SIS)-derived gel injected into infarcted myocardium 
      has been shown to promote repair and regeneration after myocardial infarction 
      (MI); however, the specific impact of SIS gel on cardiomyocytes remained unknown. 
      The aim of this study was to characterise SIS gel function in 
      hypoxia-reoxygenation (H/R)-induced cardiomyocyte damage and its potential 
      mechanism. HL-1 cardiomyocytes seeded on SIS matrix-coated plates, SIS gel, and 
      uncoated plates were subjected to H/R, cell viability, apoptosis, expression of 
      caspase-3, Bcl-2, and Bax were investigated. SIS gel and SIS matrix as coating 
      substrates markedly improved cell viability, preventing cell apoptosis compared 
      with uncoated plates, with SIS gel yielding the best cytoprotective effects. SIS 
      gel down-regulated expression of pro-inflammatory cytokines (TNF-alpha, CCL2, and 
      IL-6) by inhibiting the JNK-mitogen-activated protein kinase (MAPK)/NF-kappaB 
      pathways. Furthermore, SIS gel protected cardiomyocytes from apoptosis by 
      activating protein kinase B (AKT) and extracellular-signal-regulated kinase (ERK) 
      pathways, and markedly up-regulated antiapoptotic Bcl-2 expression but inhibited 
      that of proapoptotic Bax and c-caspase 3. Together, these findings show that SIS 
      gel could decrease H/R-induced cell apoptosis through a mechanism potentially 
      related to its ability to regulate expression of inflammatory cytokines and 
      antiapoptosis signalling pathways to prevent cell apoptosis. Our findings thereby 
      shed light on the mechanism related to SIS gel therapeutic efficacy for MI.
CI  - (c) 2019 John Wiley & Sons, Ltd.
FAU - Wang, Su-Ya
AU  - Wang SY
AUID- ORCID: 0000-0002-2825-9212
AD  - Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy 
      and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
FAU - Sang, Jiang-Wei
AU  - Sang JW
AD  - Regenerative Medicine Research Center, West China Hospital, Sichuan University, 
      Chengdu, China.
FAU - Ding, Wei
AU  - Ding W
AD  - Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy 
      and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
FAU - Qin, Ting-Wu
AU  - Qin TW
AD  - Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy 
      and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
FAU - Bai, Lin
AU  - Bai L
AD  - Regenerative Medicine Research Center, West China Hospital, Sichuan University, 
      Chengdu, China.
FAU - Zhang, Jie
AU  - Zhang J
AD  - Key Laboratory of Transplant Engineering and Immunology, Ministry of Health, West 
      China Hospital, Sichuan University, Chengdu, China.
FAU - Luo, Jing-Cong
AU  - Luo JC
AUID- ORCID: 0000-0003-3251-4105
AD  - Division of Stem Cell and Tissue Engineering, State Key Laboratory of Biotherapy 
      and Cancer Center, West China Hospital, Sichuan University, Chengdu, China.
LA  - eng
GR  - 2012JY0082/National Basic Research Program of Sichuan Province/International
GR  - 2013SZ0088/National Basic Research Program of Sichuan Province/International
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190718
PL  - England
TA  - J Tissue Eng Regen Med
JT  - Journal of tissue engineering and regenerative medicine
JID - 101308490
RN  - 0 (Gels)
RN  - S88TT14065 (Oxygen)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Cell Hypoxia/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - *Cytoprotection/drug effects
MH  - Gels/*pharmacology
MH  - Inflammation/pathology
MH  - Intestinal Mucosa/*chemistry
MH  - Intestine, Small/*chemistry
MH  - Mice
MH  - Oxygen
OTO - NOTNLM
OT  - SIS gel
OT  - apoptosis
OT  - cardiomyocyte
OT  - cytoprotection
OT  - hypoxia/reoxygenation injury
OT  - inflammation
EDAT- 2019/05/08 06:00
MHDA- 2020/06/20 06:00
CRDT- 2019/05/08 06:00
PHST- 2018/07/04 00:00 [received]
PHST- 2019/03/25 00:00 [revised]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/05/08 06:00 [pubmed]
PHST- 2020/06/20 06:00 [medline]
PHST- 2019/05/08 06:00 [entrez]
AID - 10.1002/term.2878 [doi]
PST - ppublish
SO  - J Tissue Eng Regen Med. 2019 Aug;13(8):1346-1361. doi: 10.1002/term.2878. Epub 
      2019 Jul 18.