PMID- 31065700
OWN - NLM
STAT- MEDLINE
DCOM- 20191224
LR  - 20200220
IS  - 1525-3171 (Electronic)
IS  - 0032-5791 (Linking)
VI  - 98
IP  - 10
DP  - 2019 Oct 1
TI  - Effect of a synbiotic supplement on cecal microbial ecology, antioxidant status, 
      and immune response of broiler chickens reared under heat stress.
PG  - 4408-4415
LID - 10.3382/ps/pez246 [doi]
AB  - The aim of this study was to examine the effect of a dietary synbiotic supplement 
      on the cecal microflora, antioxidant status, and immune response of broiler 
      chickens under heat stress (HS). A total of 360 one-day-old male Ross 708 broiler 
      chicks were randomly distributed among 3 dietary treatments containing a 
      synbiotic (PoultryStar consists of Bifidobacterium animalis, Enterococcus 
      faecium, Lactobacillus reuteri, Pediococcus acidilactici, and 
      fructooligosaccharides) at 0 (control), 0.5 (0.5X), and 1.0 (1.0X) g/kg. Each 
      treatment contained 8 replicates of 15 birds each housed in floor pens. Heat 
      stimulation was at 32 degrees C for 9 h daily from day 15 to 42. Heat stress-induced 
      changes of cecal bacteria were detected using bacteria-specific agars, and spleen 
      protein concentration and mRNA expression of interleukins and antioxidants were 
      examined using ELISA and real-time PCR, respectively. Under the HS condition, 
      synbiotic fed broilers regardless of dose had lower cecal enumerations of 
      Escherichia coli and coliforms, and a lower heterophil/lymphocyte (H/L) ratio (P 
      < 0.05) compared to controls. 1.0X group also had higher cecal enumerations of 
      Bifidobacterium spp. and Lactobacillus spp., spleen glutathione peroxidase (GPx), 
      and plasma nuclear factor erythroid 2-related factor 2 (Nrf-2), and a lower H/L 
      ratio compared to both control and 0.5X groups (P < 0.05). However, there were no 
      treatment effects on the levels of Enterococcus spp., the circulating monocytes, 
      eosinophils, and basophils, Toll like receptor-4 (TLR-4), interleukin-6 (IL-6), 
      interlukin-10 (IL-10), and their mRNA expression, as well as plasma Kelch-like 
      ECH-associated protein 1 (Keap-1) (P > 0.05). These results suggest that the 
      synbiotic could inhibit the negative effects of HS on broiler health through the 
      reduction of cecal pathogens, regulation of stress reactions, and improvement of 
      antioxidant status.
CI  - Published by Oxford University Press on behalf of Poultry Science Association 
      2019.
FAU - Mohammed, A A
AU  - Mohammed AA
AD  - Department of Animal Sciences, Purdue University, West Lafayette, IN 47907, USA.
AD  - Department of Animal Hygiene, Faculty of Veterinary Medicine, Assiut University, 
      Assiut 71526, Egypt.
FAU - Jiang, S
AU  - Jiang S
AD  - College of Animal Science and Technology, Southwest University, Chongqing 400715, 
      P. R. China.
FAU - Jacobs, J A
AU  - Jacobs JA
AD  - USDA Agricultural Research Service, West Lafayette, IN 47907, USA.
FAU - Cheng, H W
AU  - Cheng HW
AD  - USDA Agricultural Research Service, West Lafayette, IN 47907, USA.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Poult Sci
JT  - Poultry science
JID - 0401150
RN  - 0 (Antioxidants)
SB  - IM
MH  - Animal Nutritional Physiological Phenomena/drug effects
MH  - Animals
MH  - Antioxidants/*metabolism
MH  - Cecum/microbiology/physiology
MH  - Chickens/immunology/*metabolism/microbiology
MH  - Dose-Response Relationship, Drug
MH  - Gastrointestinal Microbiome/*drug effects/physiology
MH  - Hot Temperature/*adverse effects
MH  - Immunity, Innate/*drug effects
MH  - Male
MH  - Random Allocation
MH  - Stress, Physiological
MH  - Synbiotics/*administration & dosage
OTO - NOTNLM
OT  - antioxidant status
OT  - broiler chicken
OT  - heat stress
OT  - immunity
OT  - synbiotic
EDAT- 2019/05/09 06:00
MHDA- 2019/12/25 06:00
CRDT- 2019/05/09 06:00
PHST- 2019/04/10 00:00 [accepted]
PHST- 2019/02/13 00:00 [received]
PHST- 2019/05/09 06:00 [pubmed]
PHST- 2019/12/25 06:00 [medline]
PHST- 2019/05/09 06:00 [entrez]
AID - S0032-5791(19)47956-X [pii]
AID - 10.3382/ps/pez246 [doi]
PST - ppublish
SO  - Poult Sci. 2019 Oct 1;98(10):4408-4415. doi: 10.3382/ps/pez246.