PMID- 31067731 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20200930 IS - 2218-1989 (Print) IS - 2218-1989 (Electronic) IS - 2218-1989 (Linking) VI - 9 IP - 5 DP - 2019 May 7 TI - Postprandial Metabolic Effects of Fiber Mixes Revealed by in vivo Stable Isotope Labeling in Humans. LID - 10.3390/metabo9050091 [doi] LID - 91 AB - Food supplementation with a fiber mix of guar gum and chickpea flour represents a promising approach to reduce the risk of type 2 diabetes mellitus (T2DM) by attenuating postprandial glycemia. To investigate the effects on postprandial metabolic fluxes of glucose-derived metabolites in response to this fiber mix, a randomized, cross-over study was designed. Twelve healthy, male subjects consumed three different flatbreads either supplemented with 2% guar gum or 4% guar gum and 15% chickpea flour or without supplementation (control). The flatbreads were enriched with ~2% of (13)C-labeled wheat flour. Blood was collected at 16 intervals over a period of 360 min after bread intake and plasma samples were analyzed by GC-MS based metabolite profiling combined with stable isotope-assisted metabolomics. Although metabolite levels of the downstream metabolites of glucose, specifically lactate and alanine, were not altered in response to the fiber mix, supplementation of 4% guar gum was shown to significantly delay and reduce the exogenous formation of these metabolites. Metabolic modeling and computation of appearance rates revealed that the effects induced by the fiber mix were strongest for glucose and attenuated downstream of glucose. Further investigations to explore the potential of fiber mix supplementation to counteract the development of metabolic diseases are warranted. FAU - Schlicker, Lisa AU - Schlicker L AUID- ORCID: 0000-0002-8350-2289 AD - Department for Bioinformatics and Biochemistry, BRICS, Technische Universitat Braunschweig, Rebenring 56, 38106 Braunschweig, Germany. l.schlicker@tu-bs.de. FAU - Boers, Hanny M AU - Boers HM AD - Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands. hanny.boers@unilever.com. FAU - Dudek, Christian-Alexander AU - Dudek CA AUID- ORCID: 0000-0001-9117-7909 AD - Department for Bioinformatics and Biochemistry, BRICS, Technische Universitat Braunschweig, Rebenring 56, 38106 Braunschweig, Germany. c.dudek@tu-bs.de. FAU - Zhao, Gang AU - Zhao G AD - Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Rebenring 56, 38106 Braunschweig, Germany. gzh11@helmholtz-hzi.de. AD - Centre for Individualised Infection Medicine (CIIM), Feodor-Lynen-Strasse 15, 30625 Hannover, Germany. gzh11@helmholtz-hzi.de. FAU - Barua, Arnab AU - Barua A AD - Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Rebenring 56, 38106 Braunschweig, Germany. arnab.barua@theoretical-biology.de. AD - Centre for Individualised Infection Medicine (CIIM), Feodor-Lynen-Strasse 15, 30625 Hannover, Germany. arnab.barua@theoretical-biology.de. FAU - Trezzi, Jean-Pierre AU - Trezzi JP AUID- ORCID: 0000-0001-7418-754X AD - Integrated Biobank of Luxembourg, Luxembourg Institute of Health, 1 rue Louis Rech, 3555 Dudelange, Luxembourg. jean-pierre.trezzi@ext.uni.lu. AD - Luxembourg Centre for Systems Biomedicine, Universite du Luxembourg, 7 avenue des Hauts-Fourneaux, 4362 Esch-sur-Alzette, Luxembourg. jean-pierre.trezzi@ext.uni.lu. FAU - Meyer-Hermann, Michael AU - Meyer-Hermann M AUID- ORCID: 0000-0002-4300-2474 AD - Department of Systems Immunology and Braunschweig Integrated Centre of Systems Biology, Helmholtz Centre for Infection Research, Rebenring 56, 38106 Braunschweig, Germany. mmh@theoretical-biology.de. AD - Centre for Individualised Infection Medicine (CIIM), Feodor-Lynen-Strasse 15, 30625 Hannover, Germany. mmh@theoretical-biology.de. AD - Institute of Biochemistry, Biotechnology and Bioinformatics, Technische Universitat Braunschweig, Spielmannstrasse 7, 38106 Braunschweig, Germany. mmh@theoretical-biology.de. FAU - Jacobs, Doris M AU - Jacobs DM AD - Unilever R&D Vlaardingen, Olivier van Noortlaan 120, 3133 AT Vlaardingen, The Netherlands. doris.jacobs@unilever.com. FAU - Hiller, Karsten AU - Hiller K AD - Department for Bioinformatics and Biochemistry, BRICS, Technische Universitat Braunschweig, Rebenring 56, 38106 Braunschweig, Germany. karsten.hiller@tu-bs.de. AD - Helmholtz Centre for Infection Research, Inhoffenstrasse 7, 38124 Braunschweig, Germany. karsten.hiller@tu-bs.de. LA - eng GR - Aging and Metabolic Programming, AMPro/Helmholtz Alliance/ GR - No grant number/Unilever R&D Vlaardingen/ PT - Journal Article DEP - 20190507 PL - Switzerland TA - Metabolites JT - Metabolites JID - 101578790 PMC - PMC6571904 OTO - NOTNLM OT - 13C-enrichment OT - GC-MS OT - chickpea flour OT - guar gum OT - metabolism OT - starch OT - wheat flour COIS- H.M.B. and D.M.J. are employees of Unilever, which manufactures and markets consumer food products, including the flour used for the flatbreads in this study. EDAT- 2019/05/10 06:00 MHDA- 2019/05/10 06:01 PMCR- 2019/05/01 CRDT- 2019/05/10 06:00 PHST- 2019/04/10 00:00 [received] PHST- 2019/05/02 00:00 [revised] PHST- 2019/05/05 00:00 [accepted] PHST- 2019/05/10 06:00 [entrez] PHST- 2019/05/10 06:00 [pubmed] PHST- 2019/05/10 06:01 [medline] PHST- 2019/05/01 00:00 [pmc-release] AID - metabo9050091 [pii] AID - metabolites-09-00091 [pii] AID - 10.3390/metabo9050091 [doi] PST - epublish SO - Metabolites. 2019 May 7;9(5):91. doi: 10.3390/metabo9050091.