PMID- 31068929
OWN - NLM
STAT- MEDLINE
DCOM- 20201001
LR  - 20231011
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 10
DP  - 2019
TI  - Silica Particles Mediate Phenotypic and Functional Alteration of Dendritic Cells 
      and Induce Th2 Cell Polarization.
PG  - 787
LID - 10.3389/fimmu.2019.00787 [doi]
LID - 787
AB  - During silicosis, immune cells, including macrophages, T cells, B cells, and NK 
      cells, participate in fibrosis development through alteration of the immune 
      status. Dendritic cells (DCs) are professional antigen-presenting cells (APCs) 
      with a key role in initiating immune responses and sustaining immune tolerance to 
      maintain homeostasis. The relative contribution of DCs to silicosis progression 
      is not well-documented. In the current study, we investigated the phenotypic and 
      functional alterations of peripheral blood mononuclear cell (PBMC)-derived DCs of 
      Sprague-Dawley (SD) rat during immune responses to silica exposure. We 
      established models for direct and indirect exposure of DCs to silica by either 
      treating DCs with silica or coculturing them with alveolar macrophages (AMs) 
      treated with silica, respectively. The functional activity of DCs was analyzed by 
      measuring their expression of costimulatory molecules, fluorescent microparticle 
      uptake, cytokine production, and ability to mediate T cell polarization in vitro. 
      In vivo, we demonstrated that silica could induce DC migration in response to 
      silica exposure. Our results show that cytokine production by DCs was increased 
      in response to direct silica direct exposure, while indirect silica exposure led 
      to reduced cytokine levels. Moreover, the phagocytic capacity of DCs increased in 
      cocultures after silica exposure. Gene and protein expression analyses showed 
      that silica exposure altered the expression levels of Toll-like receptor pathway 
      proteins and inflammatory factors. DC surface expression of the costimulatory 
      molecules, CD80, CD86, and major histocompatibility complex, was inhibited by 
      exposure to silica, which mediated a Th2-polarizing response in vitro. In rats, 
      silica exposure induced migration of DCs from the peripheral blood into the 
      alveoli. These results demonstrate that direct and indirect exposure to silica 
      particles alter the phenotype and function of DCs, thereby regulating immune 
      responses. Such changes may contribute to the development of silicosis by 
      altering DC phenotype, function, and migration and by influencing the balance 
      between Th1 and Th2 cells.
FAU - Liu, Suna
AU  - Liu S
AD  - Department of Henan Newborn Screening Center, Department of Pediatrics, Third 
      Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
FAU - Hao, Changfu
AU  - Hao C
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Bao, Lei
AU  - Bao L
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Zhao, Dehua
AU  - Zhao D
AD  - Department of Henan Newborn Screening Center, Department of Pediatrics, Third 
      Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
FAU - Zhang, Hongyi
AU  - Zhang H
AD  - Hospital Infection Management, The First Affiliated Hospital of Henan University, 
      Kaifeng, China.
FAU - Hou, Jianyong
AU  - Hou J
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Wang, Di
AU  - Wang D
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Chen, Huiting
AU  - Chen H
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Feng, Feifei
AU  - Feng F
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
FAU - Yao, Wu
AU  - Yao W
AD  - Department of Public Health, Zhengzhou University, Zhengzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190424
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
RN  - 0 (Biomarkers)
RN  - 0 (Cytokines)
RN  - 0 (Particulate Matter)
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Animals
MH  - Biomarkers
MH  - Cytokines/metabolism
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Disease Models, Animal
MH  - Immunophenotyping
MH  - Leukocytes, Mononuclear/immunology/metabolism/pathology
MH  - Lymphocyte Activation/*immunology
MH  - Male
MH  - Occupational Exposure
MH  - *Particulate Matter/adverse effects
MH  - Phagocytosis
MH  - Phenotype
MH  - Rats
MH  - *Silicon Dioxide
MH  - Silicosis/etiology/metabolism/pathology
MH  - Th2 Cells/*immunology/*metabolism
PMC - PMC6491578
OTO - NOTNLM
OT  - Th2 polarization
OT  - alveolar macrophages
OT  - dendritic cells
OT  - phagocytosis
OT  - silica
OT  - silicosis
EDAT- 2019/05/10 06:00
MHDA- 2020/10/02 06:00
PMCR- 2019/01/01
CRDT- 2019/05/10 06:00
PHST- 2018/08/28 00:00 [received]
PHST- 2019/03/25 00:00 [accepted]
PHST- 2019/05/10 06:00 [entrez]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2020/10/02 06:00 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2019.00787 [doi]
PST - epublish
SO  - Front Immunol. 2019 Apr 24;10:787. doi: 10.3389/fimmu.2019.00787. eCollection 
      2019.