PMID- 31069020
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20200930
IS  - 2000-2297 (Print)
IS  - 2000-2297 (Electronic)
IS  - 2000-2297 (Linking)
VI  - 11
IP  - 1
DP  - 2019
TI  - Effect of reduced nutritional supply on the metabolic activity and survival of 
      cariogenic bacteria in vitro.
PG  - 1605788
LID - 10.1080/20002297.2019.1605788 [doi]
LID - 1605788
AB  - Sealed cariogenic bacteria are deprived from dietary carbohydrate, but could be 
      provided with nutrients by pulpal fluids, with adaptive strain-specific 
      activities being possible. We investigated survival and metabolic activity of the 
      cariogenic bacteria Streptococcus sobrinus, Actinomyces naeslundii and 
      Lactobacillus rhamnosus in different carbohydrate-limited media without carbon 
      source (CLM), or containing glucose (CLM-G), albumin (CLM-A), or alpha1-acid 
      glycoprotein (CLM-AGP) in vitro. Bacterial metabolite concentrations (lactate, 
      pyruvate, oxaloacetate, citrate, acetate, formate, ethanol, acetoin) after 20 and 
      4 hours incubation, and bacterial numbers (CFU) after 24 hours incubation were 
      analyzed using multivariate-analysis-of-variance (MANOVA). The medium 
      (p = 0.02/MANOVA), strain and incubation-time (both p < 0.001) had significant 
      impact on metabolite concentrations. Bacteria secreted mainly lactate 
      (80.3 microg/10(6) bacteria S. sobrinus) and acetate (54.5 microg/10(6) bacteria A. 
      naeslundii). Nearly all metabolites were produced in higher concentrations in S. 
      sobrinus than in A. naeslundii or L. rhamnosus (p < 0.05/HSD). Metabolite 
      concentration was significantly higher in CLM-G than in other media for most 
      metabolites (p < 0.05). L. rhamnosus showed significantly lower survival than S. 
      sobrinus and A. naeslundii (p < 0.05/HSD) regardless of the media, while S. 
      sobrinus and A. naeslundii showed medium-specific survival. Survival of carbon 
      starvation was strain- and medium-specific. Sustained organic acid production was 
      found for all strains and media.
FAU - Ganas, Petra
AU  - Ganas P
AD  - Department of Operative and Preventive Dentistry, Charite - Universitatsmedizin 
      Berlin, Berlin, Germany.
FAU - Schwendicke, Falk
AU  - Schwendicke F
AD  - Department of Operative and Preventive Dentistry, Charite - Universitatsmedizin 
      Berlin, Berlin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20190422
PL  - United States
TA  - J Oral Microbiol
JT  - Journal of oral microbiology
JID - 101551049
PMC - PMC6493303
OTO - NOTNLM
OT  - Actinomyces naeslundii
OT  - Dental caries
OT  - Lactobacillus rhamnosus
OT  - Streptococcus sobrinus
OT  - carbon metabolism
OT  - selective excavation
OT  - stress adaptation
OT  - survival rate
EDAT- 2019/05/10 06:00
MHDA- 2019/05/10 06:01
PMCR- 2019/01/01
CRDT- 2019/05/10 06:00
PHST- 2019/01/29 00:00 [received]
PHST- 2019/03/28 00:00 [revised]
PHST- 2019/04/03 00:00 [accepted]
PHST- 2019/05/10 06:00 [entrez]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2019/05/10 06:01 [medline]
PHST- 2019/01/01 00:00 [pmc-release]
AID - 1605788 [pii]
AID - 10.1080/20002297.2019.1605788 [doi]
PST - epublish
SO  - J Oral Microbiol. 2019 Apr 22;11(1):1605788. doi: 10.1080/20002297.2019.1605788. 
      eCollection 2019.