PMID- 31069605
OWN - NLM
STAT- MEDLINE
DCOM- 20200421
LR  - 20200421
IS  - 1568-5608 (Electronic)
IS  - 0925-4692 (Linking)
VI  - 27
IP  - 6
DP  - 2019 Dec
TI  - Zinc and iron complexes of oleanolic acid, (OA) attenuate allergic airway 
      inflammation in rats.
PG  - 1179-1192
LID - 10.1007/s10787-019-00597-2 [doi]
AB  - Oleanolic acid (OA) is a hydroxyl pentacyclic triterpene acid (HTAs) used in 
      various ailments. Inflammatory diseases may be profoundly influenced by iron (Fe) 
      and zinc (Zn) status. We studied the anti-asthmatic effects of two metal 
      complexes (Fe and Zn) of OA in the ovalbumin (OVA)-induced rat model. Delayed 
      type hypersensitivity (DTH) was measured. Total and differential leucocyte count 
      was done in blood as well as bronchoalveolar lavage fluid (BALF). The mRNA 
      expression levels of pro-inflammatory cytokines were measured in lung tissue by 
      reverse transcription polymerase chain reaction. The levels of cyclooxygenase-2 
      (COX-2), immunoglobulin E (IgE) and 5-lipoxygenase (5-LOX) were estimated by 
      enzyme linked immunosorbent assay. Splenocyte proliferation was performed through 
      BrdU uptake method and nitric oxide levels were measured by colorimetric assay 
      kit. The acute toxicity study was also done for the complexes. The asthmatic 
      group developed allergic airway inflammation shown by increased DTH and 
      inflammatory markers in blood and BALF. OA + Fe and OA + Zn displayed significant 
      decrease in DTH, NO, expression of IL-4, 5, 13, 17, toll-like receptor-2, nuclear 
      factor-kappa B and tumor necrosis factor-alpha; serum IgE, COX-2, and 5-LOX. The 
      metal complexes also attenuated OVA-stimulated splenocyte proliferation. While no 
      hepatotoxic or nephrotoxic potential was shown by OA + Fe and OA + Zn. Our 
      findings indicate that both OA + Fe and OA + Zn possess significant 
      anti-asthmatic effect which may be ascribed to its immunomodulatory and 
      anti-inflammatory features.
FAU - Jehangir, Adnan
AU  - Jehangir A
AD  - Department of Pharmacology, University of Health Sciences, Lahore, 54600, 
      Pakistan.
AD  - Riphah International University, Islamabad, Pakistan.
FAU - Shahzad, Muhammad
AU  - Shahzad M
AUID- ORCID: 0000-0002-4812-8957
AD  - Department of Pharmacology, University of Health Sciences, Lahore, 54600, 
      Pakistan. shahzad912@hotmail.com.
FAU - Shahid, Khadija
AU  - Shahid K
AD  - Riphah International University, Islamabad, Pakistan.
FAU - Waheed, Akbar
AU  - Waheed A
AD  - Riphah International University, Islamabad, Pakistan.
FAU - Ayub, Farhana
AU  - Ayub F
AD  - Riphah International University, Islamabad, Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20190508
PL  - Switzerland
TA  - Inflammopharmacology
JT  - Inflammopharmacology
JID - 9112626
RN  - 0 (Anti-Inflammatory Agents)
RN  - 0 (Cytokines)
RN  - 0 (Immunologic Factors)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tlr2 protein, rat)
RN  - 0 (Toll-Like Receptor 2)
RN  - 411VRN1TV4 (Nitric Acid)
RN  - 6SMK8R7TGJ (Oleanolic Acid)
RN  - E1UOL152H7 (Iron)
RN  - J41CSQ7QDS (Zinc)
SB  - IM
MH  - Animals
MH  - Anti-Inflammatory Agents/pharmacology
MH  - Asthma/*drug therapy/immunology
MH  - Cytokines/genetics
MH  - Female
MH  - Immunologic Factors/pharmacology
MH  - Iron/therapeutic use
MH  - Lymphocyte Activation/drug effects
MH  - Nitric Acid/analysis
MH  - Oleanolic Acid/pharmacology/*therapeutic use
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Toll-Like Receptor 2/physiology
MH  - Zinc/therapeutic use
OTO - NOTNLM
OT  - 5-Lipoxygenase (5-LOX)
OT  - Allergic asthma (AA)
OT  - Bronchoalveolar lavage fluid (BALF)
OT  - Cyclooxygenase-2 (COX-2)
OT  - Immunoglobulin E (IgE)
OT  - Iron (Fe)
OT  - Oleanolic acid (OA)
OT  - Zinc (Zn)
EDAT- 2019/05/10 06:00
MHDA- 2020/04/22 06:00
CRDT- 2019/05/10 06:00
PHST- 2019/01/01 00:00 [received]
PHST- 2019/04/10 00:00 [accepted]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2020/04/22 06:00 [medline]
PHST- 2019/05/10 06:00 [entrez]
AID - 10.1007/s10787-019-00597-2 [pii]
AID - 10.1007/s10787-019-00597-2 [doi]
PST - ppublish
SO  - Inflammopharmacology. 2019 Dec;27(6):1179-1192. doi: 10.1007/s10787-019-00597-2. 
      Epub 2019 May 8.