PMID- 31069688
OWN - NLM
STAT- MEDLINE
DCOM- 20191127
LR  - 20191127
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 1965
DP  - 2019
TI  - Characterization of Epigenetic Histone Activation/Repression Marks in Sequences 
      of Genes by Chromatin Immunoprecipitation-Quantitative Polymerase Chain Reaction 
      (ChIP-qPCR).
PG  - 389-403
LID - 10.1007/978-1-4939-9182-2_25 [doi]
AB  - Chromatin immunoprecipitation (ChIP) is widely used to measure protein-DNA 
      interactions. This protocol outlines a ChIP method used to identify the 
      association of a protein or protein modification (such as a specific histone 
      modification-methylation, acetylation, etc.) of interest with a specific DNA 
      sequence in a target gene in fetal mouse brains on gestational day (GD) 17. 
      Briefly, DNA and proteins are cross-linked (via formaldehyde), and chromatin is 
      sonicated into fragments between 200 and 1000 base pair (bp) long, with an 
      average length of 500 bp. The DNA-protein complexes are captured using antibodies 
      directed toward the protein or protein modification of interest. These 
      immunoprecipitated complexes are retrieved using agarose beads. The DNA-protein 
      cross-links are reversed (via heat and via presence of high salt concentrations), 
      and the ChIP DNA is purified and measured via a quantitative polymerase chain 
      (qPCR) reaction. The results show the association of histone modifications at 
      unknown sites of specific genes of interest, indicating which epigenetic 
      modifications of specific genes may be responsible for the outcome of interest.
FAU - Bhatia, Shama
AU  - Bhatia S
AD  - Department of Pharmaceutical Sciences and Centre for Pharmaceutical Oncology, 
      University of Toronto, Toronto, ON, Canada.
FAU - Matthews, Jason
AU  - Matthews J
AD  - Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, 
      Canada.
AD  - Department of Nutrition, Institute of Basic Medical Sciences, University of Oslo, 
      Oslo, Norway.
FAU - Wells, Peter G
AU  - Wells PG
AD  - Department of Pharmaceutical Sciences and Centre for Pharmaceutical Oncology, 
      University of Toronto, Toronto, ON, Canada. pg.wells@utoronto.ca.
AD  - Department of Pharmacology and Toxicology, University of Toronto, Toronto, ON, 
      Canada. pg.wells@utoronto.ca.
LA  - eng
GR  - MOP-82812/CIHR/Canada
GR  - MOP-115108/CIHR/Canada
GR  - PJT-156023/CIHR/Canada
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Chromatin)
RN  - 0 (Histones)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Acetylation
MH  - Animals
MH  - Binding Sites
MH  - Brain/*embryology/metabolism
MH  - Chromatin/genetics/metabolism
MH  - Chromatin Immunoprecipitation/*methods
MH  - DNA/chemistry/*metabolism
MH  - Epigenesis, Genetic
MH  - Histones/*metabolism
MH  - Methylation
MH  - Mice
MH  - Protein Processing, Post-Translational
MH  - Real-Time Polymerase Chain Reaction
OTO - NOTNLM
OT  - ChIP-qPCR
OT  - Chromatin immunoprecipitation (ChIP)
OT  - DNA-protein interactions
OT  - Fetal brains
OT  - Histone modifications
EDAT- 2019/05/10 06:00
MHDA- 2019/11/28 06:00
CRDT- 2019/05/10 06:00
PHST- 2019/05/10 06:00 [entrez]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2019/11/28 06:00 [medline]
AID - 10.1007/978-1-4939-9182-2_25 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2019;1965:389-403. doi: 10.1007/978-1-4939-9182-2_25.