PMID- 31070332
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20191120
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Linking)
VI  - 37
IP  - 5
DP  - 2019 May 8
TI  - [Determination of five aminoglycoside residues in honey using mixed-mode ion 
      exchange liquid chromatography-tandem mass spectrometry].
PG  - 499-504
LID - 10.3724/SP.J.1123.2018.11043 [doi]
AB  - A method using mixed-mode ion exchange liquid chromatography-tandem mass 
      spectrometry was developed for the determination of streptomycin, 
      dihydrostreptomycin, spectinomycin, kanamycin and amikacin in honey. The honey 
      samples were extracted with phosphate followed by clean-up with SupelMIP 
      Aminoglycosides SPE cartridges. The separation was performed on an SIELC Obelisc 
      R column (100 mmx2.1 mm, 5 mum) by the gradient elution program using 0.1%(v/v) 
      formic acid aqueous solution and acetonitrile as the mobile phases, at a flow 
      rate of 0.4 mL/min. Five aminoglycosides (AGs) were identified by the tandem mass 
      spectrometer with an electrospray ionization source under the multiple reaction 
      monitoring (MRM) mode. The quantification analysis was performed by the external 
      standard method. The calibration curves showed good linearity in the range of 
      5-100 mug/L for streptomycin and dihydrostreptomycin, and 20-500 mug/L for 
      spectinomycin, kanamycin and amikacin. The limits of quantification (LOQs) of the 
      five drugs in honey were 5-20 mug/kg. The average recoveries of the five drugs 
      from feeds spiked at LOQ, 2-fold LOQ and 5-fold LOQ levels were 75.1%-92.3%, and 
      the relative standard deviations (RSDs) were 4.5%-10.7%. The method is simple, 
      rapid, and sensitive, and is suitable for the simultaneous determination of the 
      five aminoglycoside residues in honey.
FAU - Wu, Yunhui
AU  - Wu Y
AD  - Xiamen Ocean Vocational College, Xiamen 361100, China.
FAU - Yan, Lijuan
AU  - Yan L
AD  - Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, 
      China.
FAU - Shen, Luying
AU  - Shen L
AD  - Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, 
      China.
FAU - Lin, Liyi
AU  - Lin L
AD  - Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, 
      China.
FAU - Zhang, Feng
AU  - Zhang F
AD  - Chinese Academy of Inspection and Quarantine, Beijing 100176, China.
FAU - Xu, Dunming
AU  - Xu D
AD  - Inspection and Quarantine Technology Center, Xiamen Customs, Xiamen 361026, 
      China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
OTO - NOTNLM
OT  - aminoglycoside
OT  - honey
OT  - mixed-mode ion exchange liquid chromatography-tandem mass spectrometry
EDAT- 2019/05/10 06:00
MHDA- 2019/05/10 06:01
CRDT- 2019/05/10 06:00
PHST- 2019/05/10 06:00 [entrez]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2019/05/10 06:01 [medline]
AID - 10.3724/SP.J.1123.2018.11043 [doi]
PST - ppublish
SO  - Se Pu. 2019 May 8;37(5):499-504. doi: 10.3724/SP.J.1123.2018.11043.