PMID- 31070893
OWN - NLM
STAT- MEDLINE
DCOM- 20200918
LR  - 20231213
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 91
IP  - 13
DP  - 2019 Jul 2
TI  - Profiling of Human Milk Oligosaccharides for Lewis Epitopes and Secretor Status 
      by Electrostatic Repulsion Hydrophilic Interaction Chromatography Coupled with 
      Negative-Ion Electrospray Tandem Mass Spectrometry.
PG  - 8199-8206
LID - 10.1021/acs.analchem.9b00687 [doi]
AB  - Human milk oligosaccharides (HMOs) are one of the most abundant ingredients in 
      breast milk, and they play a beneficial role for newborns and are important for 
      infant health. The peripheral fucosylated sequences of HMOs, such as the 
      histo-blood group ABH(O) and Lewis a, b, x, and y antigens, are determined by the 
      expression of the secretor (Se) and Lewis (Le) genes in the mammary gland, and 
      are often the recognition motifs and serve as decoy receptors for microbes. In 
      this work, we developed a method for determination of secretor status and Lewis 
      blood phenotype and assignment of Lewis blood-group epitopes. The method was 
      based on electrostatic repulsion/hydrophilic interaction chromatography coupled 
      with tandem mass spectrometry (ERLIC-MS/MS). A specifically designed stationary 
      phase, aspartic acid-bonded silica (ABS), was used to separate the acidic and 
      neutral HMOs by electrostatic repulsion followed by HILIC. Negative-ion 
      electrospray MS/MS was then used for analysis of secretor status and Lewis blood 
      phenotypes and assignment of important epitopes of HMOs from the lactating 
      mothers by selecting a specific set of unique fragment ions.
FAU - Yan, Jingyu
AU  - Yan J
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
FAU - Ding, Junjie
AU  - Ding J
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
AD  - University of Chinese Academy of Sciences , Beijing 100049 , China.
FAU - Jin, Gaowa
AU  - Jin G
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
FAU - Duan, Zhaojun
AU  - Duan Z
AD  - National Institute for Viral Disease Control and Prevention , Beijing 102206 , 
      China.
FAU - Yang, Fan
AU  - Yang F
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
FAU - Li, Dandi
AU  - Li D
AD  - National Institute for Viral Disease Control and Prevention , Beijing 102206 , 
      China.
FAU - Zhou, Han
AU  - Zhou H
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
FAU - Li, Ming
AU  - Li M
AD  - College of Basic Medical Science , Dalian Medical University , Dalian , China.
FAU - Guo, Zhimou
AU  - Guo Z
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
FAU - Chai, Wengang
AU  - Chai W
AD  - Glycosciences Laboratory , Faculty of Medicine, Imperial College London , 
      Hammersmith Campus, Du Cane Road , London W12 0NN , United Kingdom.
FAU - Liang, Xinmiao
AU  - Liang X
AUID- ORCID: 0000-0001-5802-1961
AD  - Dalian Institute of Chemical Physics , Chinese Academy of Sciences, Key 
      Laboratory of Separation Science for Analytical Chemistry , Dalian 116023 , 
      China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190522
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Lewis Blood Group Antigens)
RN  - 0 (Oligosaccharides)
RN  - EC 2.4.1.- (Fucosyltransferases)
SB  - IM
MH  - Adult
MH  - Chromatography, High Pressure Liquid
MH  - Female
MH  - Fucosyltransferases/*genetics
MH  - Humans
MH  - Hydrophobic and Hydrophilic Interactions
MH  - Lewis Blood Group Antigens/*analysis/genetics
MH  - Milk, Human/*chemistry
MH  - Oligosaccharides/*chemistry
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Galactoside 2-alpha-L-fucosyltransferase
EDAT- 2019/05/10 06:00
MHDA- 2020/09/20 06:00
CRDT- 2019/05/10 06:00
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2020/09/20 06:00 [medline]
PHST- 2019/05/10 06:00 [entrez]
AID - 10.1021/acs.analchem.9b00687 [doi]
PST - ppublish
SO  - Anal Chem. 2019 Jul 2;91(13):8199-8206. doi: 10.1021/acs.analchem.9b00687. Epub 
      2019 May 22.