PMID- 31071316
OWN - NLM
STAT- MEDLINE
DCOM- 20200609
LR  - 20200609
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 381
IP  - 2
DP  - 2019 Aug 15
TI  - Long noncoding RNA XIST increases the aggressiveness of laryngeal squamous cell 
      carcinoma by regulating miR-124-3p/EZH2.
PG  - 172-178
LID - S0014-4827(19)30225-3 [pii]
LID - 10.1016/j.yexcr.2019.04.034 [doi]
AB  - The long non-coding RNAs (lncRNAs) are an emerging class of cancer regulators. 
      The objective of the study was to elucidate the roles and underlying mechanisms 
      of XIST in laryngeal squamous cell carcinoma. Quantitative real-time PCR 
      (qRT-PCR) suggested that XIST was highly upregulated in laryngeal squamous 
      cancerous (LSCC) tissues. Knockdown of XIST, mediated by lentiviral transfection 
      of XIST-specific short-hairpin RNA (shRNA), led to the inhibition of 
      proliferation, migration, and invasion of LSCC cells in vitro. In vivo, XIST 
      knockdown also suppressed the growth of LSCC xenografts in mice. Upregulation of 
      miR-124 and downregulation of EZH2 were concomitantly observed after XIST 
      knockdown, and our data suggested that XIST served as the competitive endogenous 
      RNA of miR-124 to modulate EZH2 expression. Moreover, ectopic overexpression of 
      EZH2 prominently attenuated the anti-proliferation activity by XIST knockdown. 
      Therefore, XIST plays an important role in progression of LSCC by modulating the 
      miR-124-EZH2 axis.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Xiao, Dong
AU  - Xiao D
AD  - Department of Otolaryngology, The First Hospital, Jilin University, Changchun 
      City, Jilin Province, 130021, PR China.
FAU - Cui, Xiangyan
AU  - Cui X
AD  - Department of Otolaryngology, The First Hospital, Jilin University, Changchun 
      City, Jilin Province, 130021, PR China.
FAU - Wang, Xin
AU  - Wang X
AD  - Department of Otolaryngology, The First Hospital, Jilin University, Changchun 
      City, Jilin Province, 130021, PR China. Electronic address: wn03358@163.com.
LA  - eng
PT  - Journal Article
DEP - 20190506
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (MIRN124 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (XIST non-coding RNA)
RN  - EC 2.1.1.43 (EZH2 protein, human)
RN  - EC 2.1.1.43 (Enhancer of Zeste Homolog 2 Protein)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/*pathology
MH  - Cells, Cultured
MH  - Disease Progression
MH  - Enhancer of Zeste Homolog 2 Protein/*genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - HEK293 Cells
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/*pathology
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*genetics
MH  - Neoplasm Invasiveness
MH  - RNA, Long Noncoding/*physiology
OTO - NOTNLM
OT  - EZH2
OT  - Laryngeal squamous cell carcinoma
OT  - lncRNA XIST
OT  - miR-124-3p
EDAT- 2019/05/10 06:00
MHDA- 2020/06/10 06:00
CRDT- 2019/05/10 06:00
PHST- 2019/03/20 00:00 [received]
PHST- 2019/04/26 00:00 [revised]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2020/06/10 06:00 [medline]
PHST- 2019/05/10 06:00 [entrez]
AID - S0014-4827(19)30225-3 [pii]
AID - 10.1016/j.yexcr.2019.04.034 [doi]
PST - ppublish
SO  - Exp Cell Res. 2019 Aug 15;381(2):172-178. doi: 10.1016/j.yexcr.2019.04.034. Epub 
      2019 May 6.