PMID- 31071580
OWN - NLM
STAT- MEDLINE
DCOM- 20190613
LR  - 20190613
IS  - 1873-376X (Electronic)
IS  - 1570-0232 (Linking)
VI  - 1120
DP  - 2019 Jul 1
TI  - Oxidized glycerophosphatidylcholines in diabetes through non-targeted 
      metabolomics: Their annotation and biological meaning.
PG  - 62-70
LID - S1570-0232(19)30490-8 [pii]
LID - 10.1016/j.jchromb.2019.04.053 [doi]
AB  - Lipid oxidation is one of the most important processes occurring in living cells 
      and has been investigated through stable end-products. Currently, new insights 
      into many physiological and pathophysiological processes provide a measurement of 
      the first products of oxidation, e.g., oxidized glycerophosphatidylcholines 
      (oxGPCs). Here, we evaluate the capacity of untargeted global metabolomics to 
      measure oxGPCs in serum samples. This evaluation covered analytical 
      reproducibility and data quality as well as the ability to capture metabolic 
      alterations in diverse conditions. The analytical evaluation was performed based 
      on the quality control samples, while the comparative analysis was based on the 
      model of the development of type 2 diabetes mellitus (T2DM). The novelty of this 
      approach arises not only from the measurement of oxGPCs instead of lipid 
      peroxide-derived aldehydes but also from the stratification of the patients 
      according to body mass index (BMI). Such a scenario was dictated by the fact 
      that, despite the well-known relationship between obesity and T2DM development, 
      there are lean individuals suffering from T2DM as well as obese people with 
      normal glucose homeostasis. Our results provided evidence to support the ability 
      of nontargeted metabolomics to measure oxGPCs. Comparative analysis of measured 
      oxGPCs revealed differences in the level of oxGPCs either between different 
      stages of disease development (insulin resistance, prediabetes) or BMI groups 
      (normal weight, overweight, obese). The obtained results provided new insights 
      into the metabolic processes leading to the development of T2DM and opened new 
      paths in the investigation of the impact of body mass in T2DM progress.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Godzien, Joanna
AU  - Godzien J
AD  - Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland; 
      CEMBIO, Centre for Metabolomics and Bioanalysis, San Pablo CEU University, 
      Madrid, Spain.
FAU - Kalaska, Bartlomiej
AU  - Kalaska B
AD  - CEMBIO, Centre for Metabolomics and Bioanalysis, San Pablo CEU University, 
      Madrid, Spain; Department of Pharmacodynamics, Medical University of Bialystok, 
      Bialystok, Poland.
FAU - Adamska-Patruno, Edyta
AU  - Adamska-Patruno E
AD  - Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
FAU - Siroka, Jitka
AU  - Siroka J
AD  - CEMBIO, Centre for Metabolomics and Bioanalysis, San Pablo CEU University, 
      Madrid, Spain; Laboratory of Growth Regulators, The Czech Academy of Sciences, 
      Institute of Experimental Botany & Palacky University, Faculty of Science, 
      Olomouc, Czech Republic; Laboratory of Metabolomics, Institute of Molecular and 
      Translational Medicine, Palacky University in Olomouc, Olomouc, Czech Republic.
FAU - Ciborowski, Michal
AU  - Ciborowski M
AD  - Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
FAU - Kretowski, Adam
AU  - Kretowski A
AD  - Clinical Research Centre, Medical University of Bialystok, Bialystok, Poland.
FAU - Barbas, Coral
AU  - Barbas C
AD  - CEMBIO, Centre for Metabolomics and Bioanalysis, San Pablo CEU University, 
      Madrid, Spain. Electronic address: cbarbas@ceu.es.
LA  - eng
PT  - Journal Article
DEP - 20190429
PL  - Netherlands
TA  - J Chromatogr B Analyt Technol Biomed Life Sci
JT  - Journal of chromatography. B, Analytical technologies in the biomedical and life 
      sciences
JID - 101139554
RN  - 60M22SGW66 (Glycerylphosphorylcholine)
SB  - IM
MH  - Adult
MH  - Chromatography, Liquid
MH  - Diabetes Mellitus, Type 2/*blood/metabolism
MH  - Glycerylphosphorylcholine/*blood/*chemistry
MH  - Humans
MH  - Metabolome/*physiology
MH  - Metabolomics/*methods
MH  - Middle Aged
MH  - Oxidation-Reduction
MH  - Tandem Mass Spectrometry
OTO - NOTNLM
OT  - Long chain oxidized glycerophosphatidylcholines
OT  - Metabolomics
OT  - Oxidation
OT  - T2DM
OT  - oxGPCs
EDAT- 2019/05/10 06:00
MHDA- 2019/06/14 06:00
CRDT- 2019/05/10 06:00
PHST- 2019/03/25 00:00 [received]
PHST- 2019/04/26 00:00 [revised]
PHST- 2019/04/28 00:00 [accepted]
PHST- 2019/05/10 06:00 [pubmed]
PHST- 2019/06/14 06:00 [medline]
PHST- 2019/05/10 06:00 [entrez]
AID - S1570-0232(19)30490-8 [pii]
AID - 10.1016/j.jchromb.2019.04.053 [doi]
PST - ppublish
SO  - J Chromatogr B Analyt Technol Biomed Life Sci. 2019 Jul 1;1120:62-70. doi: 
      10.1016/j.jchromb.2019.04.053. Epub 2019 Apr 29.