PMID- 31073796 OWN - NLM STAT- MEDLINE DCOM- 20200324 LR - 20200324 IS - 1436-5073 (Electronic) IS - 0026-3672 (Linking) VI - 186 IP - 6 DP - 2019 May 9 TI - Colorimetric bio-barcode immunoassay for parathion based on amplification by using platinum nanoparticles acting as a nanozyme. PG - 339 LID - 10.1007/s00604-019-3433-6 [doi] AB - A competitive bio-barcode immunoassay is described for the trace detection of parathion in water, pear, cabbage, and rice samples. It is based on amplification by platinum nanoparticle acting as a nanozyme. Gold nanoparticles (AuNPs) were modified with (a) monoclonal antibodies (mAbs) against parathion, and (b) thiolated single-stranded DNA (ssDNA) oligonucleotides. Magnetic nanoparticles (MNPs) were functionalized with ovalbumin coupled with parathion hapten. Parathion and its hapten compete with mAbs on the surface of the AuNPs. Subsequently, the platinum nanoparticles (PtNPs) probe, which was functionalized with the complementary thiolated ssDNA (C-ssDNA), was added to the reaction mixture for the detection of parathion. The signal was catalytically amplified by coupling with platinum nanozyme using teramethylbenzidine and H(2)O(2) as the chromogenic system. The immunoassay has a linear range that extends from 0.01-50 mug.L(-1), and the limit of detection is 2.0 x 10(-3) mug.L(-1). The recoveries and relative standard deviations (RSDs) ranged from 91.1-114.4% and 3.6-15.8%, respectively. The method correlates well with data obtained by gas chromatography-tandem mass spectrometry (GC-MS/MS). Graphical abstract The parathion and the magnetic nanoparticles (MNPs) labelled with hapten-OVA competitively reacted to AuNPs modified with mAbs and thiolated DNA for the detection of parathion. The signal was catalyzed by platinum nanozyme. The limit of detection for parathion is 2.0 ng.L(-1). FAU - Chen, Ge AU - Chen G AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Jin, Maojun AU - Jin M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. katonking@163.com. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. katonking@163.com. FAU - Yan, Mengmeng AU - Yan M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Cui, Xueyan AU - Cui X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Wang, Yuanshang AU - Wang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Zheng, Weijia AU - Zheng W AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Qin, Guoxin AU - Qin G AD - Agro-Product Quality Safety and Testing Technology Research Institute, Guangxi Academy of Agricultural Sciences, Nanning, 530007, People's Republic of China. FAU - Zhang, Yudan AU - Zhang Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Li, Mingjie AU - Li M AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Liao, Yun AU - Liao Y AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Zhang, Xiuyuan AU - Zhang X AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Yan, Feiyan AU - Yan F AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. FAU - Abd El-Aty, A M AU - Abd El-Aty AM AD - Department of Pharmacology, Faculty of Veterinary Medicine, Cairo University, Giza, 12211, Egypt. AD - Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, 25240, Turkey. FAU - Hacimuftuoglu, Ahmet AU - Hacimuftuoglu A AD - Department of Medical Pharmacology, Medical Faculty, Ataturk University, Erzurum, 25240, Turkey. FAU - Wang, Jing AU - Wang J AD - Institute of Quality Standard and Testing Technology for Agro-Products, Chinese Academy of Agricultural Sciences, Beijing, 100081, People's Republic of China. w_jing2001@126.com. AD - Key Laboratory of Agro-Product Quality and Safety, Ministry of Agriculture, Beijing, 100081, People's Republic of China. w_jing2001@126.com. LA - eng GR - 2016YFD0401101/the projects of National Key Research Program of China/International GR - 31671938/National Natural Science Foundation/International GR - Y2017JC13/Special Fund for Agro-scientific Research in the Public Interest (CN)/International PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20190509 PL - Austria TA - Mikrochim Acta JT - Mikrochimica acta JID - 7808782 RN - 0 (Antibodies, Monoclonal) RN - 0 (Benzidines) RN - 0 (Pesticide Residues) RN - 0 (Water Pollutants, Chemical) RN - 059QF0KO0R (Water) RN - 3B3T5CB8EO (3,3',5,5'-tetramethylbenzidine) RN - 49DFR088MY (Platinum) RN - 61G466064D (Parathion) RN - 7440-57-5 (Gold) RN - BBX060AN9V (Hydrogen Peroxide) SB - IM MH - Antibodies, Monoclonal/immunology MH - Benzidines/chemistry MH - Brassica/chemistry MH - Catalysis MH - Colorimetry/methods MH - Gold/chemistry MH - Hydrogen Peroxide/chemistry MH - Immunoassay/*methods MH - Limit of Detection MH - Metal Nanoparticles/*chemistry MH - Oryza/chemistry MH - Parathion/*analysis/immunology MH - Pesticide Residues/analysis/immunology MH - Platinum/chemistry MH - Pyrus/chemistry MH - Water/analysis MH - Water Pollutants, Chemical/analysis/immunology OTO - NOTNLM OT - Agro-products OT - AuNPs OT - Colorimetric detection OT - DNA OT - Magnetic nanoparticles OT - Nanomaterial OT - Pesticide OT - PtNPs EDAT- 2019/05/11 06:00 MHDA- 2020/03/25 06:00 CRDT- 2019/05/11 06:00 PHST- 2018/12/20 00:00 [received] PHST- 2019/04/10 00:00 [accepted] PHST- 2019/05/11 06:00 [entrez] PHST- 2019/05/11 06:00 [pubmed] PHST- 2020/03/25 06:00 [medline] AID - 10.1007/s00604-019-3433-6 [pii] AID - 10.1007/s00604-019-3433-6 [doi] PST - epublish SO - Mikrochim Acta. 2019 May 9;186(6):339. doi: 10.1007/s00604-019-3433-6.