PMID- 31074015
OWN - NLM
STAT- MEDLINE
DCOM- 20200831
LR  - 20200831
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 120
IP  - 9
DP  - 2019 Sep
TI  - Circular RNA rno_circ_0004002 regulates cell proliferation, apoptosis, and 
      epithelial-mesenchymal transition through targeting miR-342-5p and Wnt3a in 
      anorectal malformations.
PG  - 15483-15493
LID - 10.1002/jcb.28814 [doi]
AB  - Circular RNAs (circRNAs) are a novel class of noncoding RNAs that have regulatory 
      functions in varieties of diseases. The purpose of this study was to investigate 
      the potential role of circRNAs in anorectal malformations (ARM). With sequencing 
      analysis, 78 circRNAs were identified to be differentially expressed on 
      gestational day (GD) 19 in ARM and normal anorectal tissues, including 41 
      upregulated and 37 downregulated circRNAs. Among the downregulated circRNAs, 
      rno_circ_0004002 was selected as the candidate circRNA. Functionally, silencing 
      of rno_circ_0004002 was correlated with suppression of proliferation, and 
      promotion of apoptosis and epithelial-mesenchymal transition (EMT) in anorectal 
      development. For the mechanism investigation, bioinformatic prediction and 
      luciferase reporter assay revealed that rno_circ_0004002 was proved to be a 
      sponge of miR-342-5p, and miR-342-5p could target 3'-UTR of Wnt3a to negatively 
      regulate its expression. The quantitative reverse transcription polymerase chain 
      reaction (qRT-PCR) results showed that the expression of miR-342-5p was 
      significantly increased in ARM anorectal tissues compared with normal tissues on 
      GD19 and GD21, and the expression of Wnt3a demonstrated the opposite on GD21. In 
      addition, rescue assays disclosed that miR-342-5p inhibitor could reverse the 
      role of rno_circ_0004002 knockdown on the repression of anorectal development. In 
      summary, our study shed light on the regulatory mechanism of rno_circ_0004002 in 
      cell proliferation, apoptosis, and EMT via sponging miR-342-5p, which 
      downregulated the Wnt3a expression in anorectal development. We also suggested 
      that GD19 and GD21 was the crucial time in the progression of ARM, and 
      rno_circ_0004002/miR-342-5p/Wnt3a might serve as potential therapeutic targets 
      for ARM.
CI  - (c) 2019 Wiley Periodicals, Inc.
FAU - Qu, Yuan
AU  - Qu Y
AD  - Department of Pediatric Surgery, Shengjing Hospital, China Medical University, 
      Shenyang, Liaoning, China.
FAU - Liu, Dan
AU  - Liu D
AD  - Department of Pediatric Surgery, Shengjing Hospital, China Medical University, 
      Shenyang, Liaoning, China.
FAU - Jia, Huimin
AU  - Jia H
AUID- ORCID: 0000-0001-8596-1019
AD  - Department of Pediatric Surgery, Shengjing Hospital, China Medical University, 
      Shenyang, Liaoning, China.
FAU - Yang, Zhonghua
AU  - Yang Z
AD  - Department of Pediatric Surgery, Shengjing Hospital, China Medical University, 
      Shenyang, Liaoning, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190509
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (3' Untranslated Regions)
RN  - 0 (MIRN342 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Circular)
RN  - 0 (WNT3A protein, human)
RN  - 0 (Wnt3A Protein)
SB  - IM
MH  - 3' Untranslated Regions
MH  - Animals
MH  - Anorectal Malformations/*genetics
MH  - Apoptosis
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Disease Models, Animal
MH  - Disease Progression
MH  - Epithelial-Mesenchymal Transition
MH  - Gene Expression Regulation
MH  - HEK293 Cells
MH  - Humans
MH  - MicroRNAs/*genetics
MH  - RNA, Circular/*genetics
MH  - Rats
MH  - Sequence Analysis, RNA/*methods
MH  - Wnt3A Protein/*genetics
OTO - NOTNLM
OT  - Wnt3a
OT  - anorectal malformations
OT  - circular RNA
OT  - epithelial-mesenchymal transition
OT  - miR-342-5p
OT  - rno_circ_0004002
EDAT- 2019/05/11 06:00
MHDA- 2020/09/01 06:00
CRDT- 2019/05/11 06:00
PHST- 2019/01/08 00:00 [received]
PHST- 2019/02/14 00:00 [revised]
PHST- 2019/02/27 00:00 [accepted]
PHST- 2019/05/11 06:00 [pubmed]
PHST- 2020/09/01 06:00 [medline]
PHST- 2019/05/11 06:00 [entrez]
AID - 10.1002/jcb.28814 [doi]
PST - ppublish
SO  - J Cell Biochem. 2019 Sep;120(9):15483-15493. doi: 10.1002/jcb.28814. Epub 2019 
      May 9.