PMID- 31074409
OWN - NLM
STAT- MEDLINE
DCOM- 20200121
LR  - 20200309
IS  - 1476-1645 (Electronic)
IS  - 0002-9637 (Print)
IS  - 0002-9637 (Linking)
VI  - 101
IP  - 1
DP  - 2019 Jul
TI  - Generation and Selection of a Panel of Pan-Filovirus Single-Chain Antibodies 
      using Cell-Free Ribosome Display.
PG  - 198-206
LID - 10.4269/ajtmh.18-0658 [doi]
AB  - Filoviruses, which include ebolaviruses and marburgvirus, can cause outbreaks of 
      highly lethal hemorrhagic fever. This disease causes significant morbidity and 
      mortality in humans and non-human primates, with human fatality rates reaching 
      90% during some outbreaks. Currently, there is lack of licensed vaccines or 
      antivirals for these viruses. Since early symptoms of filovirus infection mimic 
      more common diseases, there is a strong unmet public health and biodefense need 
      for broad-spectrum filovirus rapid diagnostics. We have generated a panel of 
      mouse single-chain Fv-antibodies (scFvs) to filovirus glycoproteins (GPs) using 
      cell-free ribosome display and determined their cross-reactivity profiles to all 
      known filovirus species. Two scFvs (4-2 and 22-1) were able to detect all known 
      Ebolavirus and Marburgvirus species. This is the first report on ribosome display 
      scFvs that can detect a broad set of filovirus GPs, which demonstrates the 
      potential for use in diagnostics.
FAU - Kunamneni, Adinarayana
AU  - Kunamneni A
AD  - Department of Medicine, Loyola University Medical Center, Chicago, Illinois.
AD  - Department of Internal Medicine, Center for Global Health, University of New 
      Mexico, Albuquerque, New Mexico.
FAU - Clarke, Elizabeth C
AU  - Clarke EC
AD  - Department of Internal Medicine, Center for Global Health, University of New 
      Mexico, Albuquerque, New Mexico.
FAU - Ye, Chunyan
AU  - Ye C
AD  - Department of Internal Medicine, Center for Global Health, University of New 
      Mexico, Albuquerque, New Mexico.
FAU - Bradfute, Steven B
AU  - Bradfute SB
AD  - Department of Internal Medicine, Center for Global Health, University of New 
      Mexico, Albuquerque, New Mexico.
FAU - Durvasula, Ravi
AU  - Durvasula R
AD  - Department of Internal Medicine, Center for Global Health, University of New 
      Mexico, Albuquerque, New Mexico.
AD  - Department of Medicine, Loyola University Medical Center, Chicago, Illinois.
LA  - eng
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PL  - United States
TA  - Am J Trop Med Hyg
JT  - The American journal of tropical medicine and hygiene
JID - 0370507
RN  - 0 (Antibodies, Viral)
RN  - 0 (Single-Chain Antibodies)
RN  - 0 (Viral Proteins)
SB  - IM
MH  - Animals
MH  - Antibodies, Viral/*physiology
MH  - Antibody Affinity
MH  - Antibody Specificity
MH  - Cell-Free System
MH  - Cloning, Molecular
MH  - Filoviridae/*immunology
MH  - HEK293 Cells
MH  - Humans
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Ribosomes
MH  - Single-Chain Antibodies
MH  - Viral Proteins/immunology
PMC - PMC6609206
EDAT- 2019/05/11 06:00
MHDA- 2020/01/22 06:00
PMCR- 2019/05/06
CRDT- 2019/05/11 06:00
PHST- 2019/05/11 06:00 [pubmed]
PHST- 2020/01/22 06:00 [medline]
PHST- 2019/05/11 06:00 [entrez]
PHST- 2019/05/06 00:00 [pmc-release]
AID - tpmd180658 [pii]
AID - 10.4269/ajtmh.18-0658 [doi]
PST - ppublish
SO  - Am J Trop Med Hyg. 2019 Jul;101(1):198-206. doi: 10.4269/ajtmh.18-0658.