PMID- 31075255
OWN - NLM
STAT- MEDLINE
DCOM- 20200615
LR  - 20200615
IS  - 1090-2422 (Electronic)
IS  - 0014-4827 (Linking)
VI  - 381
IP  - 1
DP  - 2019 Aug 1
TI  - Recombinant Treponema pallidum protein Tp47 promotes the migration and adherence 
      of THP-1 cells to human dermal vascular smooth muscle cells by inducing MCP-1 and 
      ICAM-1 expression.
PG  - 150-162
LID - S0014-4827(19)30226-5 [pii]
LID - 10.1016/j.yexcr.2019.04.035 [doi]
AB  - Vascular inflammation is a complex and multifactorial pathophysiological process 
      that plays a crucial role in all stages of syphilis and is responsible for tissue 
      damage. Little is known about the interactions of infiltrating immunocytes with 
      human dermal vascular smooth muscle cells (HDVSMCs) in arterioles during the 
      immunopathogenesis of syphilis. The Treponema pallidum subsp. pallidum membrane 
      protein Tp47 is considered a major inducer of inflammation initiation and 
      development. In this study, we demonstrated that Tp47 promoted the migration and 
      adhesion of THP-1 cells to HDVSMCs. Furthermore, Tp47 increased monocyte 
      chemoattractant protein-1 (MCP-1) and intercellular adhesion molecule-1 (ICAM-1) 
      mRNA and protein expression levels in a dose- and time-dependent manner. The 
      migration and adhesion of THP-1 cells to HDVSMCs were significantly suppressed by 
      anti-MCP-1 and anti-ICAM-1 neutralizing antibodies, respectively. Further studies 
      revealed that treatment of HDVSMCs with Tp47 activated the PI3K/Akt, p38 MAPK and 
      NF-kappaB signalling pathways. Inhibition of PI3K/Akt, p38 MAPK and NF-kappaB suppressed 
      the MCP-1 and ICAM-1 expression induced by Tp47. In addition, the migration and 
      adhesion of THP-1 cells to Tp47-treated HDVSMCs were significantly decreased by 
      pretreatment with PI3K/Akt, p38 MAPK and NF-kappaB inhibitors. These findings 
      demonstrate that Tp47 promotes the migration and adherence of THP-1 cells to 
      HDVSMCs by inducing MCP-1 and ICAM-1 expression, which is mediated by activation 
      of the PI3K/Akt, p38 MAPK and NF-kappaB pathways. This study provides a novel 
      potential therapeutic strategy for controlling the vascular inflammatory response 
      in syphilis patients.
CI  - Copyright (c) 2019. Published by Elsevier Inc.
FAU - Gao, Zheng-Xiang
AU  - Gao ZX
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China; Institute of Infectious Disease, School of 
      Medicine, Xiamen University, Xiamen, 361004, China.
FAU - Liu, Dan
AU  - Liu D
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China.
FAU - Liu, Li-Li
AU  - Liu LL
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China; Institute of Infectious Disease, School of 
      Medicine, Xiamen University, Xiamen, 361004, China.
FAU - Lin, Li-Rong
AU  - Lin LR
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China; Institute of Infectious Disease, School of 
      Medicine, Xiamen University, Xiamen, 361004, China.
FAU - Tong, Man-Li
AU  - Tong ML
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China.
FAU - Niu, Jian-Jun
AU  - Niu JJ
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China; Institute of Infectious Disease, School of 
      Medicine, Xiamen University, Xiamen, 361004, China. Electronic address: 
      niujianjun211@xmu.edu.cn.
FAU - Yang, Tian-Ci
AU  - Yang TC
AD  - Center of Clinical Laboratory, Zhongshan Hospital, School of Medicine, Xiamen 
      University, Xiamen, 361004, China; Institute of Infectious Disease, School of 
      Medicine, Xiamen University, Xiamen, 361004, China. Electronic address: 
      yangtianci@xmu.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190507
PL  - United States
TA  - Exp Cell Res
JT  - Experimental cell research
JID - 0373226
RN  - 0 (CCL2 protein, human)
RN  - 0 (Chemokine CCL2)
RN  - 0 (ICAM1 protein, human)
RN  - 0 (NF-kappa B)
RN  - 0 (Recombinant Proteins)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - EC 3.5.2.6 (Tp47 protein, Treponema pallidum)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - Cell Adhesion
MH  - Cell Movement
MH  - Cells, Cultured
MH  - Chemokine CCL2/metabolism
MH  - Dermis/metabolism/pathology
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Muscle, Smooth, Vascular/*metabolism/pathology
MH  - NF-kappa B/metabolism
MH  - Phosphorylation
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Recombinant Proteins
MH  - Signal Transduction
MH  - Syphilis/metabolism/*microbiology/pathology
MH  - THP-1 Cells
MH  - Treponema pallidum/*physiology
MH  - beta-Lactamases/genetics/*physiology
MH  - p38 Mitogen-Activated Protein Kinases/metabolism
OTO - NOTNLM
OT  - Chemoattractant protein-1
OT  - Human dermal vascular smooth muscle cells
OT  - Intercellular adhesion molecule-1
OT  - THP-1 cells
OT  - Treponema pallidum
EDAT- 2019/05/11 06:00
MHDA- 2020/06/17 06:00
CRDT- 2019/05/11 06:00
PHST- 2019/01/27 00:00 [received]
PHST- 2019/04/25 00:00 [revised]
PHST- 2019/04/29 00:00 [accepted]
PHST- 2019/05/11 06:00 [pubmed]
PHST- 2020/06/17 06:00 [medline]
PHST- 2019/05/11 06:00 [entrez]
AID - S0014-4827(19)30226-5 [pii]
AID - 10.1016/j.yexcr.2019.04.035 [doi]
PST - ppublish
SO  - Exp Cell Res. 2019 Aug 1;381(1):150-162. doi: 10.1016/j.yexcr.2019.04.035. Epub 
      2019 May 7.