PMID- 31080068
OWN - NLM
STAT- MEDLINE
DCOM- 20200211
LR  - 20200211
IS  - 1097-4172 (Electronic)
IS  - 0092-8674 (Linking)
VI  - 177
IP  - 5
DP  - 2019 May 16
TI  - Rational Engineering of XCaMPs, a Multicolor GECI Suite for In Vivo Imaging of 
      Complex Brain Circuit Dynamics.
PG  - 1346-1360.e24
LID - S0092-8674(19)30393-9 [pii]
LID - 10.1016/j.cell.2019.04.007 [doi]
AB  - To decipher dynamic brain information processing, current genetically encoded 
      calcium indicators (GECIs) are limited in single action potential (AP) detection 
      speed, combinatorial spectral compatibility, and two-photon imaging depth. To 
      address this, here, we rationally engineered a next-generation quadricolor GECI 
      suite, XCaMPs. Single AP detection was achieved within 3-10 ms of spike onset, 
      enabling measurements of fast-spike trains in parvalbumin (PV)-positive 
      interneurons in the barrel cortex in vivo and recording three distinct (two 
      inhibitory and one excitatory) ensembles during pre-motion activity in freely 
      moving mice. In vivo paired recording of pre- and postsynaptic firing revealed 
      spatiotemporal constraints of dendritic inhibition in layer 1 in vivo, between 
      axons of somatostatin (SST)-positive interneurons and apical tufts dendrites of 
      excitatory pyramidal neurons. Finally, non-invasive, subcortical imaging using 
      red XCaMP-R uncovered somatosensation-evoked persistent activity in hippocampal 
      CA1 neurons. Thus, the XCaMPs offer a critical enhancement of solution space in 
      studies of complex neuronal circuit dynamics. VIDEO ABSTRACT.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Inoue, Masatoshi
AU  - Inoue M
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Bioengineering, Stanford 
      University School of Medicine, Stanford, CA 94305, USA.
FAU - Takeuchi, Atsuya
AU  - Takeuchi A
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Manita, Satoshi
AU  - Manita S
AD  - Department of Neurophysiology, Faculty of Medicine, University of Yamanashi, 
      Chuo, Yamanashi 409-3898, Japan.
FAU - Horigane, Shin-Ichiro
AU  - Horigane SI
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neuroscience I, Research 
      Institute of Environmental Medicine, Nagoya University, Nagoya, Aichi 464-8601, 
      Japan; Department of Molecular/Cellular Neuroscience, Nagoya University Graduate 
      School of Medicine, Nagoya, Aichi 466-8550, Japan.
FAU - Sakamoto, Masayuki
AU  - Sakamoto M
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Kawakami, Ryosuke
AU  - Kawakami R
AD  - Laboratory of Molecular and Cellular Biophysics, Research Institute for 
      Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; 
      Department of Molecular Medicine for Pathogenesis, Ehime University Graduate 
      School of Medicine, Toon, Ehime 791-0295, Japan.
FAU - Yamaguchi, Kazushi
AU  - Yamaguchi K
AD  - Laboratory of Molecular and Cellular Biophysics, Research Institute for 
      Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
FAU - Otomo, Kouhei
AU  - Otomo K
AD  - Laboratory of Molecular and Cellular Biophysics, Research Institute for 
      Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan.
FAU - Yokoyama, Hiroyuki
AU  - Yokoyama H
AD  - New Industry Creation Hatchery Center (NICHe), Tohoku University, Sendai, Miyagi 
      980-8579, Japan.
FAU - Kim, Ryang
AU  - Kim R
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Yokoyama, Tatsushi
AU  - Yokoyama T
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Takemoto-Kimura, Sayaka
AU  - Takemoto-Kimura S
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neuroscience I, Research 
      Institute of Environmental Medicine, Nagoya University, Nagoya, Aichi 464-8601, 
      Japan; Department of Molecular/Cellular Neuroscience, Nagoya University Graduate 
      School of Medicine, Nagoya, Aichi 466-8550, Japan; Precursory Research for 
      Embryonic Science and Technology (PRESTO), Japan Science and Technology Agency, 
      Saitama, Japan.
FAU - Abe, Manabu
AU  - Abe M
AD  - Department of Cellular Neurobiology, Brain Research Institute, Niigata 
      University, Niigata, Niigata 951-8585, Japan.
FAU - Okamura, Michiko
AU  - Okamura M
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Kondo, Yayoi
AU  - Kondo Y
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Quirin, Sean
AU  - Quirin S
AD  - Department of Bioengineering, Stanford University School of Medicine, Stanford, 
      CA 94305, USA.
FAU - Ramakrishnan, Charu
AU  - Ramakrishnan C
AD  - Department of Bioengineering, Stanford University School of Medicine, Stanford, 
      CA 94305, USA.
FAU - Imamura, Takeshi
AU  - Imamura T
AD  - Department of Molecular Medicine for Pathogenesis, Ehime University Graduate 
      School of Medicine, Toon, Ehime 791-0295, Japan.
FAU - Sakimura, Kenji
AU  - Sakimura K
AD  - Department of Cellular Neurobiology, Brain Research Institute, Niigata 
      University, Niigata, Niigata 951-8585, Japan.
FAU - Nemoto, Tomomi
AU  - Nemoto T
AD  - Laboratory of Molecular and Cellular Biophysics, Research Institute for 
      Electronic Science, Hokkaido University, Sapporo, Hokkaido 001-0020, Japan; Core 
      Research for Evolutional Science and Technology (CREST), Japan Agency for Medical 
      Research and Development, Tokyo, Japan.
FAU - Kano, Masanobu
AU  - Kano M
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; International Research Center for 
      Neurointelligence, The University of Tokyo, Tokyo, Japan.
FAU - Fujii, Hajime
AU  - Fujii H
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan.
FAU - Deisseroth, Karl
AU  - Deisseroth K
AD  - Department of Bioengineering, Stanford University School of Medicine, Stanford, 
      CA 94305, USA.
FAU - Kitamura, Kazuo
AU  - Kitamura K
AD  - Department of Neurophysiology, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; Department of Neurophysiology, Faculty 
      of Medicine, University of Yamanashi, Chuo, Yamanashi 409-3898, Japan.
FAU - Bito, Haruhiko
AU  - Bito H
AD  - Department of Neurochemistry, Graduate School of Medicine, The University of 
      Tokyo, Bunkyo-ku, Tokyo 113-0033, Japan; International Research Center for 
      Neurointelligence, The University of Tokyo, Tokyo, Japan. Electronic address: 
      hbito@m.u-tokyo.ac.jp.
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190509
PL  - United States
TA  - Cell
JT  - Cell
JID - 0413066
SB  - IM
MH  - Action Potentials/*physiology
MH  - Animals
MH  - Axons/*metabolism
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Female
MH  - Hippocampus/cytology/*metabolism
MH  - Interneurons/cytology/*metabolism
MH  - Mice
MH  - Mice, Transgenic
MH  - Pyramidal Cells/cytology/*metabolism
MH  - Rats
MH  - Rats, Sprague-Dawley
OTO - NOTNLM
OT  - PV recording
OT  - XCaMP
OT  - ckkap sequence
OT  - genetically encoded calcium indicators
OT  - multiplex imaging
OT  - non-invasive hippocampal recording
OT  - paired pre- and post-synapse recording
OT  - two-photon Ca(2+) imaging
EDAT- 2019/05/14 06:00
MHDA- 2020/02/12 06:00
CRDT- 2019/05/14 06:00
PHST- 2018/11/19 00:00 [received]
PHST- 2019/02/28 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/05/14 06:00 [pubmed]
PHST- 2020/02/12 06:00 [medline]
PHST- 2019/05/14 06:00 [entrez]
AID - S0092-8674(19)30393-9 [pii]
AID - 10.1016/j.cell.2019.04.007 [doi]
PST - ppublish
SO  - Cell. 2019 May 16;177(5):1346-1360.e24. doi: 10.1016/j.cell.2019.04.007. Epub 
      2019 May 9.