PMID- 31081043
OWN - NLM
STAT- MEDLINE
DCOM- 20190830
LR  - 20200225
IS  - 1791-2423 (Electronic)
IS  - 1019-6439 (Print)
IS  - 1019-6439 (Linking)
VI  - 54
IP  - 6
DP  - 2019 Jun
TI  - TGF-beta-induced long non-coding RNA MIR155HG promotes the progression and EMT of 
      laryngeal squamous cell carcinoma by regulating the miR-155-5p/SOX10 axis.
PG  - 2005-2018
LID - 10.3892/ijo.2019.4784 [doi]
AB  - Non‑coding RNAs, particularly long non‑coding RNAs (lncRNAs), play important 
      roles in tumorigenesis. The miR‑155 host gene (MIR155HG) lncRNA has been found to 
      play a crucial role in tumor progression. However, the role of MIR155HG in 
      laryngeal squamous cell carcinoma (LSCC) remains unclear. Thus, the aim of the 
      present study was to explore the roles and underlying molecular mechanisms of 
      action of MIR155HG and miR‑155‑5p in LSCC, in an effort to provide novel 
      approaches for the antitumor therapy for LSCC. In the present study, the 
      expression levels of miR‑155‑5p and MIR155HG were detected by reverse 
      tran-scription‑quantitative polymerase chain reaction. In addition, the 
      biological functions of MIR155HG and miR‑155‑5p on LSCC were evaluated in vitro 
      by MTS assay, colony formation assay and Transwell assays, and in vivo by 
      tumorigenesis assays. It was revealed that MIR155HG and miR‑155‑5p were 
      significantly upregulated in LSCC tissues, and were associated with the TNM 
      stage, pathological differentiation and lymph node metastasis. Moreover, the 
      knockdown of MIR155HG and miR‑155‑5p inhibited the proliferation, migration and 
      invasion of LSCC cells, whereas their overexpression exerted the opposite effects 
      in vitro and MIR155HG overexpression promoted tumorigenesis in vivo. Furthermore, 
      MIR155HG downregulation reduced the expression level of miR‑155‑5p. The 
      inhibitory effect of MIR155HG knockdown on malignant behavior was abrogated by 
      miR‑155‑5p overexpression. Bioinformatics analysis and luciferase reporter assay 
      confirmed that miR‑155‑5p contributed to the progression of LSCC by directly 
      binding to the 3' untranslated region of SRY‑related‑HMG‑box 10 (SOX10). In 
      addition, MIR155HG and miR‑155‑5p were upregulated by the induction of 
      transforming growth factor‑beta (TGF‑beta) and promoted the expression of mesenchymal 
      markers synergistically. On the whole, the findings of the present study indicate 
      a novel role of MIR155HG in the TGF‑beta‑induced EMT of LSCC cells by regulating EMT 
      markers through the miR‑155/SOX10 axis. The MIR155HG/miR‑155‑5p/SOX10 axis plays 
      an important role in promoting the progression of LSCC and may thus serve as a 
      potential therapeutic target for LSCC treatment.
FAU - Cui, Weina
AU  - Cui W
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Meng, Wenxia
AU  - Meng W
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Zhao, Lei
AU  - Zhao L
AD  - Department of Otorhinolaryngology, The Affiliated Hospital of Hebei University, 
      Baoding, Hebei 071000, P.R. China.
FAU - Cao, Huan
AU  - Cao H
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
FAU - Chi, Weiwei
AU  - Chi W
AD  - Department of Otorhinolaryngology, The First Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050031, P.R. China.
FAU - Wang, Baoshan
AU  - Wang B
AD  - Department of Otorhinolaryngology, The Second Hospital of Hebei Medical 
      University, Shijiazhuang, Hebei 050000, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190412
PL  - Greece
TA  - Int J Oncol
JT  - International journal of oncology
JID - 9306042
RN  - 0 (MIRN155 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (SOX10 protein, human)
RN  - 0 (SOXE Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
SB  - IM
MH  - Animals
MH  - Carcinoma, Squamous Cell/genetics/metabolism/*pathology
MH  - Cell Line, Tumor
MH  - Cell Proliferation
MH  - Disease Progression
MH  - Epithelial-Mesenchymal Transition
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Laryngeal Neoplasms/genetics/metabolism/*pathology
MH  - Lymphatic Metastasis
MH  - Male
MH  - Mice
MH  - MicroRNAs/*genetics
MH  - Neoplasm Staging
MH  - Neoplasm Transplantation
MH  - RNA, Long Noncoding/*genetics
MH  - SOXE Transcription Factors/*genetics
MH  - Transforming Growth Factor beta/*metabolism
MH  - Up-Regulation
PMC - PMC6521927
EDAT- 2019/05/14 06:00
MHDA- 2019/08/31 06:00
PMCR- 2019/04/12
CRDT- 2019/05/14 06:00
PHST- 2018/10/26 00:00 [received]
PHST- 2019/04/05 00:00 [accepted]
PHST- 2019/05/14 06:00 [entrez]
PHST- 2019/05/14 06:00 [pubmed]
PHST- 2019/08/31 06:00 [medline]
PHST- 2019/04/12 00:00 [pmc-release]
AID - ijo-54-06-2005 [pii]
AID - 10.3892/ijo.2019.4784 [doi]
PST - ppublish
SO  - Int J Oncol. 2019 Jun;54(6):2005-2018. doi: 10.3892/ijo.2019.4784. Epub 2019 Apr 
      12.