PMID- 31081107
OWN - NLM
STAT- MEDLINE
DCOM- 20200909
LR  - 20200909
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 8
DP  - 2019 Apr
TI  - Influence of lncRNA HOTAIR on acute kidney injury in sepsis rats through 
      regulating miR-34a/Bcl-2 pathway.
PG  - 3512-3519
LID - 17717 [pii]
LID - 10.26355/eurrev_201904_17717 [doi]
AB  - OBJECTIVE: To study the regulatory effect of long non-coding ribonucleic acid 
      (lncRNA) HOX transcript antisense RNA (HOTAIR) on acute kidney injury (AKI) in 
      sepsis rats and its regulatory mechanism. MATERIALS AND METHODS: The 
      sepsis-induced AKI model was established in Sprague-Dawley (SD) male rats through 
      cecal ligation puncture. A total of 30 SD rats were randomly divided into the 
      control group, model group and lncRNA HOTAIR mimic group, with 10 rats in each 
      group. Relative levels of tumor necrosis factor-alpha (TNF-alpha) and interleukin-1beta 
      (IL-1beta) in kidney tissues were detected via enzyme-linked immunosorbent assay 
      (ELISA). Apoptosis of kidney tissues was detected via terminal deoxynucleotidyl 
      transferase-mediated dUTP nick end labeling (TUNEL) staining. Moreover, the 
      target gene of miR-34a was searched using the miRNA online database. The 
      messenger RNA (mRNA) expression levels of miR-34a and B-cell lymphoma-2 (Bcl-2) 
      were detected via Reverse Transcription-Polymerase Chain Reaction (RT-PCR). 
      RESULTS: Compared with those in the control group, the rats in the model group 
      showed injured pathological morphology of kidney, elevated contents of TNF-alpha and 
      IL-1beta, and apoptosis in kidney tissues. The target gene of miR-34a was Bcl-2, 
      according to the miRNA online database. MiR-34a level in kidney tissues was 
      upregulated, while the mRNA level of Bcl-2 significantly decreased in the model 
      group. Compared with those in the model group, the pathological morphology of 
      kidney tissues was improved, the content of TNF-alpha and IL-1beta markedly declined, 
      and the apoptotic rate of kidney tissues also reduced in lncRNA HOTAIR mimic 
      group. The miR-34a level in kidney tissues decreased, while the Bcl-2 mRNA level 
      remarkably increased in lncRNA HOTAIR mimic group. CONCLUSIONS: LncRNA HOTAIR 
      overexpression can alleviate AKI in sepsis rats by inhibiting the apoptosis of 
      kidney tissues by downregulating the miR-34a/Bcl-2 signaling pathway.
FAU - Jiang, Z-J
AU  - Jiang ZJ
AD  - Department of Emergency, Qingpu Branch of Zhongshan Hospital Affiliated to Fudan 
      University, Shanghai, China. 15821363280@163.com.
FAU - Zhang, M-Y
AU  - Zhang MY
FAU - Fan, Z-W
AU  - Fan ZW
FAU - Sun, W-L
AU  - Sun WL
FAU - Tang, Y
AU  - Tang Y
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (Bcl2 protein, rat)
RN  - 0 (HOTAIR long untranslated RNA, rat)
RN  - 0 (MIRN34 microRNA, rat)
RN  - 0 (MicroRNAs)
RN  - 0 (Proto-Oncogene Proteins c-bcl-2)
RN  - 0 (RNA, Long Noncoding)
SB  - IM
MH  - Acute Kidney Injury/*genetics/pathology
MH  - Animals
MH  - Apoptosis/genetics
MH  - Disease Models, Animal
MH  - Humans
MH  - Kidney/pathology
MH  - Male
MH  - MicroRNAs/*genetics/metabolism
MH  - Proto-Oncogene Proteins c-bcl-2/*genetics/metabolism
MH  - RNA, Long Noncoding/*metabolism
MH  - Rats
MH  - Sepsis/*complications
MH  - Signal Transduction/genetics
MH  - Up-Regulation
EDAT- 2019/05/14 06:00
MHDA- 2020/09/10 06:00
CRDT- 2019/05/14 06:00
PHST- 2019/05/14 06:00 [entrez]
PHST- 2019/05/14 06:00 [pubmed]
PHST- 2020/09/10 06:00 [medline]
AID - 17717 [pii]
AID - 10.26355/eurrev_201904_17717 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Apr;23(8):3512-3519. doi: 
      10.26355/eurrev_201904_17717.