PMID- 31081112
OWN - NLM
STAT- MEDLINE
DCOM- 20200909
LR  - 20200909
IS  - 2284-0729 (Electronic)
IS  - 1128-3602 (Linking)
VI  - 23
IP  - 8
DP  - 2019 Apr
TI  - LncRNA SNHG20 promotes the development of laryngeal squamous cell carcinoma by 
      regulating miR-140.
PG  - 3401-3409
LID - 17704 [pii]
LID - 10.26355/eurrev_201904_17704 [doi]
AB  - OBJECTIVE: This study aims to investigate the expression level of long non-coding 
      RNA (lncRNA) SNHG20 in laryngeal squamous cell carcinoma (LSCC), and to explore 
      further whether it can promote the development of LSCC by regulating microRNA-140 
      (miR-140). PATIENTS AND METHODS: Expression levels of SNHG20 in 56 pairs of LSCC 
      tissues and adjacent normal tissues were measured by quantitative Real 
      Time-Polymerase Chain Reaction (qRT-PCR). The relationship between SNHG20 
      expression with pathological parameters and the prognosis of LSCC was analyzed. 
      Besides, the SNHG20 expression in LSCC cells was also analyzed by qRT-PCR. The 
      SNHG20 knockdown and overexpression model were constructed by lentivirus 
      transfection in AMC-HN-8 and Hep-2 cells. Cell counting kit-8 (CCK-8) and 
      5-Ethynyl-2'-deoxyuridine (EdU) assay were used to analyze the effect of SNHG20 
      on the biological function of LSCC cells. Finally, the dual-luciferase reporter 
      gene assay was performed to explore the potentials of SNHG20 and miR-140 in LSCC. 
      RESULTS: The SNHG20 expression in LSCC tissues or cells remarkably increased than 
      controls, and the difference was statistically significant. The LSCC patients 
      with the high expression level of SNHG20 were more likely to develop advanced 
      tumor compared with patients with low expression of SNHG20. Moreover, the LSCC 
      patients with the high expression level of SNHG20 had a shorter overall survival 
      than those with low level. The cell proliferation ability significantly decreased 
      in the SNHG20 knockdown group, while notably increased in SNHG20 overexpression 
      group. MiR-140 was negatively correlated with SNHG20 in LSCC tissues and cells. 
      Dual-luciferase reporter gene assay showed that SNHG20 could be targeted by 
      miR-140 through a certain binding site. The cell rescue experiment also indicated 
      that there was a mutual regulation between SNHG20 and miR-140, which could 
      together affect the malignant progression of LSCC. CONCLUSIONS: We showed that 
      the expression levels of SNHG20 in LSCC tissues or cell lines significantly 
      increased and was associated with advanced tumor staging and undesirable 
      prognosis of LSCC. In addition, SNHG20 could promote the malignant progression of 
      LSCC.
FAU - Li, Y
AU  - Li Y
AD  - Department of Otorhinolaryngology, Second Affiliated Hospital, School of 
      Medicine, Zhejiang University, Hangzhou, China. yangbb1959@sina.com.
FAU - Xu, J
AU  - Xu J
FAU - Guo, Y-N
AU  - Guo YN
FAU - Yang, B-B
AU  - Yang BB
LA  - eng
PT  - Journal Article
PL  - Italy
TA  - Eur Rev Med Pharmacol Sci
JT  - European review for medical and pharmacological sciences
JID - 9717360
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn140 microRNA, human)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (long non-coding RNA SNHG20, human)
SB  - IM
MH  - Cell Proliferation/genetics
MH  - Disease Progression
MH  - Female
MH  - *Gene Expression Regulation, Neoplastic
MH  - Gene Knockdown Techniques
MH  - Humans
MH  - Laryngeal Mucosa/pathology/surgery
MH  - Laryngeal Neoplasms/*genetics/mortality/pathology/surgery
MH  - Laryngectomy
MH  - Male
MH  - MicroRNAs/*genetics
MH  - Middle Aged
MH  - Neoplasm Staging
MH  - RNA, Long Noncoding/genetics/*metabolism
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/mortality/pathology/surgery
MH  - Up-Regulation
EDAT- 2019/05/14 06:00
MHDA- 2020/09/10 06:00
CRDT- 2019/05/14 06:00
PHST- 2019/05/14 06:00 [entrez]
PHST- 2019/05/14 06:00 [pubmed]
PHST- 2020/09/10 06:00 [medline]
AID - 17704 [pii]
AID - 10.26355/eurrev_201904_17704 [doi]
PST - ppublish
SO  - Eur Rev Med Pharmacol Sci. 2019 Apr;23(8):3401-3409. doi: 
      10.26355/eurrev_201904_17704.