PMID- 31083497
OWN - NLM
STAT- MEDLINE
DCOM- 20191101
LR  - 20211204
IS  - 1660-3397 (Electronic)
IS  - 1660-3397 (Linking)
VI  - 17
IP  - 5
DP  - 2019 May 11
TI  - Protective Effect of Pyropia yezoensis Peptide on Dexamethasone-Induced Myotube 
      Atrophy in C2C12 Myotubes.
LID - 10.3390/md17050284 [doi]
LID - 284
AB  - Dexamethasone (DEX), a synthetic glucocorticoid, causes skeletal muscle atrophy. 
      This study examined the protective effects of Pyropia yezoensis peptide (PYP15) 
      against DEX-induced myotube atrophy and its association with insulin-like growth 
      factor-I (IGF-I) and the Akt/mammalian target of rapamycin (mTOR)-forkhead box O 
      (FoxO) signaling pathway. To elucidate the molecular mechanisms underlying the 
      effects of PYP15 on DEX-induced myotube atrophy, C2C12 myotubes were treated for 
      24 h with 100 muM DEX in the presence or absence of 500 ng/mL PYP15. Cell 
      viability assays revealed no PYP15 toxicity in C2C12 myotubes. PYP15 activated 
      the insulin-like growth factor-I receptor (IGF-IR) and Akt-mTORC1 signaling 
      pathway in DEX-induced myotube atrophy. In addition, PYP15 markedly downregulated 
      the nuclear translocation of transcription factors FoxO1 and FoxO3a, and 
      inhibited 20S proteasome activity. Furthermore, PYP15 inhibited the 
      autophagy-lysosomal pathway in DEX-stimulated myotube atrophy. Our findings 
      suggest that PYP15 treatment protected against myotube atrophy by regulating 
      IGF-I and the Akt-mTORC1-FoxO signaling pathway in skeletal muscle. Therefore, 
      PYP15 treatment appears to exert protective effects against skeletal muscle 
      atrophy.
FAU - Lee, Min-Kyeong
AU  - Lee MK
AUID- ORCID: 0000-0002-7292-8743
AD  - Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea. 
      3633234@hanmail.net.
FAU - Choi, Jeong-Wook
AU  - Choi JW
AD  - Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea. 
      wook8309@naver.com.
FAU - Choi, Youn Hee
AU  - Choi YH
AUID- ORCID: 0000-0002-0091-7893
AD  - Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea. 
      unichoi@pknu.ac.kr.
AD  - Department of Marine Bio-Materials & Aquaculture, Pukyong National University, 
      Busan 48513, Korea. unichoi@pknu.ac.kr.
FAU - Nam, Taek-Jeong
AU  - Nam TJ
AUID- ORCID: 0000-0002-5954-6935
AD  - Institute of Fisheries Sciences, Pukyong National University, Busan 46041, Korea. 
      namtj@pknu.ac.kr.
AD  - Department of Food Science and Nutrition, Pukyong National University, Busan 
      48513, Korea. namtj@pknu.ac.kr.
LA  - eng
GR  - 2012R1A6A1028677/National Research Foundation of Korea/
PT  - Journal Article
DEP - 20190511
PL  - Switzerland
TA  - Mar Drugs
JT  - Marine drugs
JID - 101213729
RN  - 0 (Insulin Receptor Substrate Proteins)
RN  - 0 (Irs1 protein, mouse)
RN  - 0 (Peptides)
RN  - 0 (Plant Proteins)
RN  - 0 (insulin-like growth factor-1, mouse)
RN  - 67763-96-6 (Insulin-Like Growth Factor I)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.7.1.1 (mTOR protein, mouse)
RN  - EC 2.7.11.1 (Proto-Oncogene Proteins c-akt)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Autophagy/drug effects
MH  - Cell Line
MH  - Cell Survival/drug effects
MH  - Dexamethasone/pharmacology/*toxicity
MH  - Insulin Receptor Substrate Proteins/metabolism
MH  - Insulin-Like Growth Factor I/metabolism
MH  - Lysosomes/metabolism
MH  - Mice
MH  - Muscle Fibers, Skeletal/*drug effects/metabolism/pathology
MH  - Muscle, Skeletal/drug effects
MH  - Muscular Atrophy/chemically induced/*drug therapy/metabolism/pathology
MH  - Peptides/chemistry/*pharmacology
MH  - Plant Proteins/chemistry/*pharmacology
MH  - Proto-Oncogene Proteins c-akt/metabolism
MH  - Rhodophyta/*chemistry
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC6563069
OTO - NOTNLM
OT  - PYP15
OT  - Pyropia yezoensis peptide
OT  - dexamethasone
OT  - myotube atrophy
OT  - protein synthesis
OT  - proteolytic system
COIS- The authors declare no conflict of interest.
EDAT- 2019/05/15 06:00
MHDA- 2019/11/02 06:00
PMCR- 2019/05/01
CRDT- 2019/05/15 06:00
PHST- 2019/04/11 00:00 [received]
PHST- 2019/05/09 00:00 [revised]
PHST- 2019/05/10 00:00 [accepted]
PHST- 2019/05/15 06:00 [entrez]
PHST- 2019/05/15 06:00 [pubmed]
PHST- 2019/11/02 06:00 [medline]
PHST- 2019/05/01 00:00 [pmc-release]
AID - md17050284 [pii]
AID - marinedrugs-17-00284 [pii]
AID - 10.3390/md17050284 [doi]
PST - epublish
SO  - Mar Drugs. 2019 May 11;17(5):284. doi: 10.3390/md17050284.