PMID- 31086021
OWN - NLM
STAT- MEDLINE
DCOM- 20190902
LR  - 20200225
IS  - 1420-3049 (Electronic)
IS  - 1420-3049 (Linking)
VI  - 24
IP  - 9
DP  - 2019 May 13
TI  - Development of an Accelerated Solvent Extraction-Ultra-Performance Liquid 
      Chromatography-Fluorescence Detection Method for Quantitative Analysis of 
      Thiamphenicol, Florfenicol and Florfenicol Amine in Poultry Eggs.
LID - 10.3390/molecules24091830 [doi]
LID - 1830
AB  - A simple, rapid and novel method for the detection of residues of thiamphenicol 
      (TAP), florfenicol (FF) and its metabolite, florfenicol amine (FFA), in poultry 
      eggs by ultra-performance liquid chromatography-fluorescence detection (UPLC-FLD) 
      was developed. The samples were extracted with acetonitrile-ammonia (98:2, v/v) 
      using accelerated solvent extraction (ASE) and purified by manual degreasing with 
      acetonitrile-saturated n-hexane. The target compounds were separated on an 
      ACQUITY UPLC((R)) BEH C(18) (2.1 mm x 100 mm, 1.7 mum) chromatographic column using 
      a mobile phase composed of 0.005 mol/L NaH(2)PO(4), 0.003 mol/L sodium lauryl 
      sulfate and 0.05% trimethylamine, adjusted to pH 5.3 +/- 0.1 by phosphoric acid and 
      acetonitrile (64:36, v/v). The limits of detection (LODs) and limits of 
      quantification (LOQs) of the three target compounds in poultry eggs were 1.8-4.9 
      microg/kg and 4.3-11.7 microg/kg, respectively. The recoveries of the three target 
      compounds in poultry eggs were above 80.1% when the spiked concentrations of 
      three phenicols were the LOQ, 0.5 maximum residue limit (MRL), 1.0 MRL and 2.0 
      MRL. The intraday relative standard deviations (RSDs) were less than 5.5%, and 
      the interday RSDs were less than 6.6%. Finally, this new detection method was 
      successfully applied to the quantitative analysis of TAP, FF and FFA in 150 
      commercial poultry eggs.
FAU - Wang, Bo
AU  - Wang B
AD  - College of Veterinary Medicine, Yangzhou University, Yangzhou 225009, China. 
      yzwbo168@163.com.
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. yzwbo168@163.com.
FAU - Xie, Xing
AU  - Xie X
AD  - Key Laboratory of Veterinary Biological Engineering and Technology, Ministry of 
      Agriculture, Institute of Veterinary Medicine, Jiangsu Academy of Agricultural 
      Sciences, Nanjing 210014, China. yzxx1989@163.com.
FAU - Zhao, Xia
AU  - Zhao X
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. 18252711481@139.com.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. 18252711481@139.com.
FAU - Xie, Kaizhou
AU  - Xie K
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. yzxkz168@163.com.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. yzxkz168@163.com.
FAU - Diao, Zhixiang
AU  - Diao Z
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. 18352764521@163.com.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. 18352764521@163.com.
FAU - Zhang, Genxi
AU  - Zhang G
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. zgx1588@126.com.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. zgx1588@126.com.
FAU - Zhang, Tao
AU  - Zhang T
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. zhangt@yzu.edu.cn.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. zhangt@yzu.edu.cn.
FAU - Dai, Guojun
AU  - Dai G
AD  - Joint International Research Laboratory of Agriculture & Agri-Product Safety, 
      Yangzhou University, Yangzhou 225009, China. gjdai@163.com.
AD  - College of Animal Science and Technology, Yangzhou University, Yangzhou 225009, 
      China. gjdai@163.com.
LA  - eng
GR  - CARS-41-G23/China Agriculture Research System/
GR  - PAPD/the Priority Academic Programme Development of Jiangsu Higher Education 
      Institutions/
GR  - 31800161/the National Natural and Science Foundation of China/
PT  - Journal Article
DEP - 20190513
PL  - Switzerland
TA  - Molecules
JT  - Molecules (Basel, Switzerland)
JID - 100964009
RN  - 0 (Solvents)
RN  - 76639-93-5 (florfenicol amine)
RN  - 9J97307Y1H (florfenicol)
RN  - FLQ7571NPM (Thiamphenicol)
SB  - IM
MH  - Animals
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Eggs/*analysis
MH  - Limit of Detection
MH  - Poultry
MH  - Solvents/*chemistry
MH  - Spectrometry, Fluorescence/*methods
MH  - Thiamphenicol/*analogs & derivatives/*analysis
PMC - PMC6539316
OTO - NOTNLM
OT  - ASE
OT  - UPLC-FLD
OT  - florfenicol
OT  - florfenicol amine
OT  - poultry eggs
OT  - thiamphenicol
COIS- The authors declare no conflicts of interest.
EDAT- 2019/05/16 06:00
MHDA- 2019/09/03 06:00
PMCR- 2019/05/13
CRDT- 2019/05/16 06:00
PHST- 2019/04/13 00:00 [received]
PHST- 2019/05/05 00:00 [revised]
PHST- 2019/05/10 00:00 [accepted]
PHST- 2019/05/16 06:00 [entrez]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2019/09/03 06:00 [medline]
PHST- 2019/05/13 00:00 [pmc-release]
AID - molecules24091830 [pii]
AID - molecules-24-01830 [pii]
AID - 10.3390/molecules24091830 [doi]
PST - epublish
SO  - Molecules. 2019 May 13;24(9):1830. doi: 10.3390/molecules24091830.