PMID- 31086573
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 17
IP  - 6
DP  - 2019 Jun
TI  - PM2.5 induces cell cycle arrest through regulating mTOR/P70S6K1 signaling 
      pathway.
PG  - 4371-4378
LID - 10.3892/etm.2019.7466 [doi]
AB  - Fine particulate matter (PM2.5) pollution has become a serious problem in China. 
      This study aims to elucidate the toxicity mechanism of PM2.5. Protein levels were 
      detected by western blotting and RT-qPCR, and cell cycle was detected by flow 
      cytometry. The results showed that exposure to PM2.5 induces cell cycle arrest 
      and downregulation of the expression of cyclin D1 protein. Moreover, the protein 
      expression of thymidylate synthase (TS) enzyme was found to be downregulated and 
      the mRNA expression of TS was upregulated after PM2.5 exposure. Knockout of TS 
      gene promoted cell cycle arrest and downregulation of the expression of cyclin D1 
      protein after PM2.5 exposure. Our data further revealed that PM2.5 exposure 
      downregulates the expression of TS and cyclin D1 partially through the 
      downregulation of the mammalian target of rapamycin (mTOR)/P70S6K1 signaling 
      pathway. Thus, these findings indicate that PM2.5-induced cell cycle arrest might 
      be due to the downregulation of mTOR/P70S6K1 signaling pathway, and thus inhibits 
      the expression of TS protein.
FAU - Zhang, Yu
AU  - Zhang Y
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
FAU - Yang, Dan
AU  - Yang D
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
AD  - Department of Pharmacology, Shenyang Medical College, Shenyang, Liaoning 110034, 
      P.R. China.
FAU - Yang, Biao
AU  - Yang B
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
FAU - Li, Bingyu
AU  - Li B
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
FAU - Guo, Jie
AU  - Guo J
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
FAU - Xiao, Chunling
AU  - Xiao C
AD  - Key Lab of Environmental Pollution and Microecology of Liaoning Province, 
      Shenyang, Liaoning 110034, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190403
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6489014
OTO - NOTNLM
OT  - PM2.5
OT  - cell cycle arrest
OT  - cyclin D1 protein
OT  - mTOR/P70S6K1 signaling pathway
OT  - thymidylate synthase protein
EDAT- 2019/05/16 06:00
MHDA- 2019/05/16 06:01
PMCR- 2019/04/03
CRDT- 2019/05/16 06:00
PHST- 2018/06/11 00:00 [received]
PHST- 2019/02/04 00:00 [accepted]
PHST- 2019/05/16 06:00 [entrez]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2019/05/16 06:01 [medline]
PHST- 2019/04/03 00:00 [pmc-release]
AID - ETM-0-0-7466 [pii]
AID - 10.3892/etm.2019.7466 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Jun;17(6):4371-4378. doi: 10.3892/etm.2019.7466. Epub 2019 Apr 
      3.