PMID- 31086583
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 1792-0981 (Print)
IS  - 1792-1015 (Electronic)
IS  - 1792-0981 (Linking)
VI  - 17
IP  - 6
DP  - 2019 Jun
TI  - Effects of hypoxia ischemia on caspase-3 expression and neuronal apoptosis in the 
      brain of neonatal mice.
PG  - 4517-4521
LID - 10.3892/etm.2019.7487 [doi]
AB  - Effects of hypoxia ischemia on caspase-3 expression and neuronal apoptosis in the 
      brain of neonatal mice were investigated. Twenty-five neonatal CD1 mice aged 1 
      week were selected and randomly divided into sham-operation group (n=8) and 
      newborn hypoxia ischemia encephalopathy (NHIE) model group (n=17). The messenger 
      ribonucleic acid (mRNA) expression levels of caspase-3 and Fas ligand (FasL) in 
      brain tissues of mice in both groups were detected via reverse 
      transcription-polymerase chain reaction (RT-PCR). The protein expression levels 
      of caspase-3 and FasL in mice in both groups were detected via western blotting. 
      Moreover, apoptosis of brain tissues was detected using the terminal 
      deoxynucleotidyl transferase-mediated dUTP nick end labeling (TUNEL), and 
      caspase-3 protein expression level in brain tissues was detected using 
      immunohistochemical methods. Results of RT-PCR and western blotting revealed that 
      compared with those in sham-operation group, caspase-3 and FasL expression levels 
      in model group were significantly increased. Results of TUNEL showed that the 
      number of apoptotic neurons in model group was significantly increased. Besides, 
      results of immunohistochemical detection manifested that the caspase-3 protein 
      expression level in model group was obviously increased. Hypoxia ischemia can 
      lead to significant increase of caspase-3 expression and increase of neuronal 
      apoptosis in the brain of neonatal mice.
FAU - Deng, Changbo
AU  - Deng C
AD  - Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, Guangdong 510700, P.R. China.
FAU - Li, Juan
AU  - Li J
AD  - Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, Guangdong 510700, P.R. China.
FAU - Li, Luyi
AU  - Li L
AD  - Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, Guangdong 510700, P.R. China.
FAU - Sun, Fengjie
AU  - Sun F
AD  - Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, Guangdong 510700, P.R. China.
FAU - Xie, Jiqing
AU  - Xie J
AD  - Department of Pediatrics, The Fifth Affiliated Hospital of Guangzhou Medical 
      University, Guangzhou, Guangdong 510700, P.R. China.
LA  - eng
PT  - Journal Article
DEP - 20190415
PL  - Greece
TA  - Exp Ther Med
JT  - Experimental and therapeutic medicine
JID - 101531947
PMC - PMC6488988
OTO - NOTNLM
OT  - caspase-3
OT  - hypoxia ischemia
OT  - neuronal apoptosis
EDAT- 2019/05/16 06:00
MHDA- 2019/05/16 06:01
PMCR- 2019/04/15
CRDT- 2019/05/16 06:00
PHST- 2018/06/18 00:00 [received]
PHST- 2019/02/01 00:00 [accepted]
PHST- 2019/05/16 06:00 [entrez]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2019/05/16 06:01 [medline]
PHST- 2019/04/15 00:00 [pmc-release]
AID - ETM-0-0-7487 [pii]
AID - 10.3892/etm.2019.7487 [doi]
PST - ppublish
SO  - Exp Ther Med. 2019 Jun;17(6):4517-4521. doi: 10.3892/etm.2019.7487. Epub 2019 Apr 
      15.