PMID- 31089162
OWN - NLM
STAT- MEDLINE
DCOM- 20201021
LR  - 20231012
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 9
IP  - 1
DP  - 2019 May 14
TI  - Simple Surface Modification of Poly(dimethylsiloxane) via Surface Segregating 
      Smart Polymers for Biomicrofluidics.
PG  - 7377
LID - 10.1038/s41598-019-43625-5 [doi]
LID - 7377
AB  - Poly(dimethylsiloxane) (PDMS) is likely the most popular material for 
      microfluidic devices in lab-on-a-chip and other biomedical applications. However, 
      the hydrophobicity of PDMS leads to non-specific adsorption of proteins and other 
      molecules such as therapeutic drugs, limiting its broader use. Here, we introduce 
      a simple method for preparing PDMS materials to improve hydrophilicity and 
      decrease non-specific protein adsorption while retaining cellular 
      biocompatibility, transparency, and good mechanical properties without the need 
      for any post-cure surface treatment. This approach utilizes smart copolymers 
      comprised of poly(ethylene glycol) (PEG) and PDMS segments (PDMS-PEG) that, when 
      blended with PDMS during device manufacture, spontaneously segregate to surfaces 
      in contact with aqueous solutions and reduce the hydrophobicity without any added 
      manufacturing steps. PDMS-PEG-modified PDMS samples showed contact angles as low 
      as 23.6 degrees  +/- 1 degrees  and retained this hydrophilicity for at least twenty months. Their 
      improved wettability was confirmed using capillary flow experiments. Modified 
      devices exhibited considerably reduced non-specific adsorption of albumin, 
      lysozyme, and immunoglobulin G. The modified PDMS was biocompatible, displaying 
      no adverse effects when used in a simple liver-on-a-chip model using primary rat 
      hepatocytes. This PDMS modification method can be further applied in analytical 
      separations, biosensing, cell studies, and drug-related studies.
FAU - Gokaltun, Aslihan
AU  - Gokaltun A
AD  - Center for Engineering in Medicine at Massachusetts General Hospital, Harvard 
      Medical School, and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 
      02114, USA.
AD  - Department of Chemical and Biological Engineering, Tufts University, 4 Colby 
      Street, Medford, MA, 02474, USA.
AD  - Department of Chemical Engineering, Hacettepe University, 06532, Beytepe, Ankara, 
      Turkey.
FAU - Kang, Young Bok Abraham
AU  - Kang YBA
AUID- ORCID: 0000-0002-8562-3797
AD  - Center for Engineering in Medicine at Massachusetts General Hospital, Harvard 
      Medical School, and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 
      02114, USA.
FAU - Yarmush, Martin L
AU  - Yarmush ML
AD  - Center for Engineering in Medicine at Massachusetts General Hospital, Harvard 
      Medical School, and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 
      02114, USA.
AD  - Department of Biomedical Engineering, Rutgers University, 599 Taylor Rd., 
      Piscataway, NJ, 08854, USA.
FAU - Usta, O Berk
AU  - Usta OB
AUID- ORCID: 0000-0001-9328-568X
AD  - Center for Engineering in Medicine at Massachusetts General Hospital, Harvard 
      Medical School, and Shriners Hospital for Children, 51 Blossom St., Boston, MA, 
      02114, USA. berkusta@gmail.com.
FAU - Asatekin, Ayse
AU  - Asatekin A
AD  - Department of Chemical and Biological Engineering, Tufts University, 4 Colby 
      Street, Medford, MA, 02474, USA. ayse.asatekin@tufts.edu.
LA  - eng
GR  - P41 EB002503/EB/NIBIB NIH HHS/United States
GR  - R21 EB020192/EB/NIBIB NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20190514
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (Dimethylpolysiloxanes)
RN  - 0 (Stimuli Responsive Polymers)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 63148-62-9 (baysilon)
SB  - IM
MH  - Animals
MH  - Cells, Cultured
MH  - Dimethylpolysiloxanes/*chemistry
MH  - Hepatocytes
MH  - Hydrophobic and Hydrophilic Interactions
MH  - *Lab-On-A-Chip Devices
MH  - Materials Testing
MH  - Microfluidic Analytical Techniques/*instrumentation
MH  - Polyethylene Glycols/chemistry
MH  - Primary Cell Culture
MH  - Rats
MH  - Stimuli Responsive Polymers/*chemistry
MH  - Surface Properties
PMC - PMC6517421
COIS- The authors declare no competing interests.
EDAT- 2019/05/16 06:00
MHDA- 2020/10/22 06:00
PMCR- 2019/05/14
CRDT- 2019/05/16 06:00
PHST- 2018/12/19 00:00 [received]
PHST- 2019/04/09 00:00 [accepted]
PHST- 2019/05/16 06:00 [entrez]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2020/10/22 06:00 [medline]
PHST- 2019/05/14 00:00 [pmc-release]
AID - 10.1038/s41598-019-43625-5 [pii]
AID - 43625 [pii]
AID - 10.1038/s41598-019-43625-5 [doi]
PST - epublish
SO  - Sci Rep. 2019 May 14;9(1):7377. doi: 10.1038/s41598-019-43625-5.