PMID- 31089421
OWN - NLM
STAT- MEDLINE
DCOM- 20191220
LR  - 20220408
IS  - 1942-0994 (Electronic)
IS  - 1942-0900 (Print)
IS  - 1942-0994 (Linking)
VI  - 2019
DP  - 2019
TI  - Endogenous CSE/Hydrogen Sulfide System Regulates the Effects of Glucocorticoids 
      and Insulin on Muscle Protein Synthesis.
PG  - 9752698
LID - 10.1155/2019/9752698 [doi]
LID - 9752698
AB  - AIMS: Insulin and glucocorticoids play crucial roles in skeletal muscle protein 
      turnover. Fast-twitch glycolytic fibres are more susceptible to atrophy than 
      slow-twitch oxidative fibres. Based on accumulating evidence, hydrogen sulfide 
      (H(2)S) is a physiological mediator of this process. The regulatory effect of 
      H(2)S on protein synthesis in fast-twitch fibres was evaluated. RESULTS: A NaHS 
      (sodium hydrosulfide) injection simultaneously increased the diameter of M. 
      pectoralis major (i.e., fast-twitch glycolytic fibres) and activated the 
      mammalian target of the rapamycin (mTOR)/p70S6 kinase (p70S6K) pathway. 
      Dexamethasone (DEX) inhibited protein synthesis, downregulated mTOR and p70S6K 
      phosphorylation, and suppressed the expression of the cystathionine gamma-lyase (CSE) 
      protein in myoblasts. The precursor of H(2)S, L-cysteine, completely abolished 
      the inhibitory effects of DEX. The CSE inhibitor DL-propargylglycine (PAG) 
      completely abrogated the effects of RU486 on blocking the suppressive effects of 
      DEX. The H(2)S donor NaHS increased the H(2)S concentrations and abrogated the 
      inhibitory effects of DEX on protein synthesis. Insulin increased protein 
      synthesis and upregulated CSE expression. However, PAG abrogated the stimulatory 
      effects of insulin on protein synthesis and the activity of the mTOR/p70S6K 
      pathway. INNOVATION: These results demonstrated that CSE/H(2)S regulated protein 
      synthesis in fast-twitch muscle fibres, and glucocorticoids and insulin regulated 
      protein synthesis in an endogenous CSE/H(2)S system-dependent manner. 
      CONCLUSIONS: The results from the present study suggest that the endogenous 
      CSE/H(2)S system regulates fast-twitch glycolytic muscle degeneration and 
      regeneration.
FAU - Wang, Ruxia
AU  - Wang R
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Li, Kelin
AU  - Li K
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Wang, Hui
AU  - Wang H
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Jiao, Hongchao
AU  - Jiao H
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Wang, Xiaojuan
AU  - Wang X
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Zhao, Jingpeng
AU  - Zhao J
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
FAU - Lin, Hai
AU  - Lin H
AUID- ORCID: 0000-0002-0878-137X
AD  - Shandong Provincial Key Laboratory of Animal Biotechnology and Disease Control 
      and Prevention, Shandong Agricultural University, 61 Daizong Street, Taian City, 
      Shandong Province 271018, China.
LA  - eng
PT  - Journal Article
DEP - 20190407
PL  - United States
TA  - Oxid Med Cell Longev
JT  - Oxidative medicine and cellular longevity
JID - 101479826
RN  - 0 (Alkynes)
RN  - 0 (Glucocorticoids)
RN  - 0 (Insulin)
RN  - 0 (Muscle Proteins)
RN  - 0 (Receptors, Glucocorticoid)
RN  - 320T6RNW1F (Mifepristone)
RN  - 64165-64-6 (propargylglycine)
RN  - 7S5I7G3JQL (Dexamethasone)
RN  - EC 2.7.11.1 (Ribosomal Protein S6 Kinases, 70-kDa)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 4.4.1.1 (Cystathionine gamma-Lyase)
RN  - K848JZ4886 (Cysteine)
RN  - TE7660XO1C (Glycine)
RN  - YY9FVM7NSN (Hydrogen Sulfide)
SB  - IM
MH  - Alkynes/pharmacology
MH  - Animals
MH  - Chickens
MH  - Cystathionine gamma-Lyase/*metabolism
MH  - Cysteine/pharmacology
MH  - Dexamethasone/pharmacology
MH  - Glucocorticoids/*pharmacology
MH  - Glycine/analogs & derivatives/pharmacology
MH  - Hydrogen Sulfide/*metabolism
MH  - Injections, Intraperitoneal
MH  - Insulin/*pharmacology
MH  - Mifepristone/pharmacology
MH  - Muscle Proteins/*biosynthesis
MH  - Myoblasts/drug effects/metabolism
MH  - Phosphorylation/drug effects
MH  - Protein Biosynthesis/*drug effects
MH  - Receptors, Glucocorticoid/metabolism
MH  - Ribosomal Protein S6 Kinases, 70-kDa/metabolism
MH  - Signal Transduction/drug effects
MH  - TOR Serine-Threonine Kinases/metabolism
PMC - PMC6476024
EDAT- 2019/05/16 06:00
MHDA- 2019/12/21 06:00
PMCR- 2019/04/07
CRDT- 2019/05/16 06:00
PHST- 2018/09/04 00:00 [received]
PHST- 2018/11/09 00:00 [revised]
PHST- 2018/12/18 00:00 [accepted]
PHST- 2019/05/16 06:00 [entrez]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2019/12/21 06:00 [medline]
PHST- 2019/04/07 00:00 [pmc-release]
AID - 10.1155/2019/9752698 [doi]
PST - epublish
SO  - Oxid Med Cell Longev. 2019 Apr 7;2019:9752698. doi: 10.1155/2019/9752698. 
      eCollection 2019.