PMID- 31091483
OWN - NLM
STAT- MEDLINE
DCOM- 20200108
LR  - 20200108
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 133
DP  - 2019 Jul 15
TI  - Development of a flow cytometric assay to assess the bacterial count in boar 
      semen.
PG  - 125-134
LID - S0093-691X(18)30882-3 [pii]
LID - 10.1016/j.theriogenology.2019.04.036 [doi]
AB  - The aim of the study was to develop a new flow cytometric assay for the 
      determination of the bacterial count in commercially processed boar semen. In 
      total 224 fresh boar semen samples collected at an AI-station were analyzed. The 
      number of total viable counts (TVC) was determined by using flow cytometry after 
      staining with SYBR Green I and Propidium Iodide (PI). In the first part of the 
      study 111 fresh boar semen samples were spiked with pure cultures of defined 
      numbers of bacteria commonly detected in boar ejaculates and analyzed by flow 
      cytometry. In the second part, 113 fresh semen samples were assessed on the day 
      of collection through flow cytometry and the Most Probable Number (MPN) method, 
      as the standard bacteriological method. The first part of the study showed a 
      strong correlation between the detected and expected numbers (r = 0.96; 
      P < 0.001), while in the second part of the study the TVC determined by flow 
      cytometry and by the MPN method correlated only moderately (r = 0.28; P < 0.01; 
      median MPN: 24,000 +/- MAD 21,600 bacteria/mL; median flow cytometry: 24,426 +/- MAD 
      15,610 bacteria/mL). In summary flow cytometry is a fast alternative to the 
      classical culture technique to determine highly contaminated boar ejaculates. The 
      developed flow cytometric protocol enables one to enumerate the viable bacteria 
      within fresh boar ejaculates without requiring numerous treatment steps, and thus 
      offering the possibility of an on-line use in AI-centers.
CI  - Copyright (c) 2019 Elsevier Inc. All rights reserved.
FAU - Oehler, Christin
AU  - Oehler C
AD  - Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland. Electronic address: coehler@vetclinics.uzh.ch.
FAU - Janett, Fredi
AU  - Janett F
AD  - Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
FAU - Schmitt, Sarah
AU  - Schmitt S
AD  - Institute of Veterinary Bacteriology, Vetsuisse Faculty, University of Zurich, 
      Zurich, Switzerland.
FAU - Malama, Eleni
AU  - Malama E
AD  - Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
FAU - Bollwein, Heinrich
AU  - Bollwein H
AD  - Clinic of Reproductive Medicine, Vetsuisse Faculty, University of Zurich, Zurich, 
      Switzerland.
LA  - eng
PT  - Journal Article
DEP - 20190430
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Animals
MH  - Flow Cytometry/methods/veterinary
MH  - Male
MH  - Semen/*microbiology
MH  - Semen Analysis/methods/*veterinary
MH  - Swine/*microbiology
OTO - NOTNLM
OT  - Bacteria
OT  - Boar
OT  - Flow cytometry
OT  - Semen
EDAT- 2019/05/16 06:00
MHDA- 2020/01/09 06:00
CRDT- 2019/05/16 06:00
PHST- 2018/10/23 00:00 [received]
PHST- 2019/04/25 00:00 [revised]
PHST- 2019/04/30 00:00 [accepted]
PHST- 2019/05/16 06:00 [pubmed]
PHST- 2020/01/09 06:00 [medline]
PHST- 2019/05/16 06:00 [entrez]
AID - S0093-691X(18)30882-3 [pii]
AID - 10.1016/j.theriogenology.2019.04.036 [doi]
PST - ppublish
SO  - Theriogenology. 2019 Jul 15;133:125-134. doi: 
      10.1016/j.theriogenology.2019.04.036. Epub 2019 Apr 30.