PMID- 31091745
OWN - NLM
STAT- MEDLINE
DCOM- 20191113
LR  - 20231012
IS  - 1424-8220 (Electronic)
IS  - 1424-8220 (Linking)
VI  - 19
IP  - 10
DP  - 2019 May 14
TI  - Self-Assembly of Functional Nucleic Acid-Based Colorimetric Competition Assay for 
      the Detection of Immunoglobulin E.
LID - 10.3390/s19102224 [doi]
LID - 2224
AB  - In this work, we have developed a simple and rapid colorimetric assay for the 
      detection of immunoglobulin E (IgE) using functional nucleic acids (FNAs) and a 
      solid-phase competition enzyme-linked immunosorbent assay (ELISA). The FNAs 
      including aptamer of recombinant IgE, G-quadruplex and its complementary 
      fragments were immobilized on 96-well microplates to achieve recognition and 
      detection of IgE in biological samples. The G-quadruplex DNAzyme catalyzed 
      2,2'-Azino-bis (3-ethylbenzothiazoline-6-sulfonic acid (ABTS)-hemin-H(2)O(2) 
      system was used to improve the sensitivity of colorimetric assay. In the presence 
      of IgE, the hairpin structure and G-quadruplex would be destroyed, resulting in 
      the inactivation of DNAzyme and subsequent reduction of its absorbance. This 
      cost-effective approach detected IgE in the linear range from 5.0 pg/mL to 500 
      ng/mL, with the limit of detection (LOD) of 2.0 pg/mL, under optimal conditions. 
      Moreover, the developed method was successfully applied to the rapid detection of 
      IgE in human urine, indicating a great potentiality of this approach in clinical 
      diagnosis and other biomedical applications.
FAU - Lin, Xuexia
AU  - Lin X
AUID- ORCID: 0000-0002-4761-6844
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. 
      linxuexia@hqu.edu.cn.
FAU - Yu, Caiyun
AU  - Yu C
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. 
      caiyunYu001@163.com.
FAU - Lin, Honggui
AU  - Lin H
AD  - School of Marine Engineering, Jimei University, Xiamen 361021 China. 
      linhongui36@163.com.
FAU - Wang, Cui
AU  - Wang C
AD  - Applied and Environment Microbiology, Department of Biology, Georgie State 
      University, Atlanta, GA 30303, USA. lorna.cui@hotmail.com.
FAU - Su, Jianlong
AU  - Su J
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. 
      sujianlong_123@163.com.
FAU - Cheng, Jie
AU  - Cheng J
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. 
      chengj@hqu.edu.cn.
FAU - Kankala, Ranjith Kumar
AU  - Kankala RK
AUID- ORCID: 0000-0003-4081-9179
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. 
      ranjithkankala@hqu.edu.cn.
FAU - Zhou, Shu-Feng
AU  - Zhou SF
AUID- ORCID: 0000-0002-5542-6119
AD  - Department of Chemical Engineering & Pharmaceutical Engineering, College of 
      Chemical Engineering, Huaqiao University, Xiamen 361021, China. szhou@hqu.edu.cn.
LA  - eng
GR  - 21804045, 21275059 and 21575044/National Natural Science Foundation of China/
GR  - 2018J05020, and 2016J01062/Natural Science Foundation of Fujian Province/
GR  - 3502Z20183027, and 3502Z20183040/Natural Science Foundation of Xiamen City/
GR  - ZQN-PY612/Promotion Program for Young and Middle-aged Teacher in Science and 
      Technology Research of Huaqiao University/
PT  - Journal Article
DEP - 20190514
PL  - Switzerland
TA  - Sensors (Basel)
JT  - Sensors (Basel, Switzerland)
JID - 101204366
RN  - 0 (Benzothiazoles)
RN  - 0 (DNA, Catalytic)
RN  - 0 (Sulfonic Acids)
RN  - 28752-68-3 (2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid)
RN  - 37341-29-0 (Immunoglobulin E)
RN  - BBX060AN9V (Hydrogen Peroxide)
SB  - IM
MH  - Benzothiazoles/chemistry
MH  - *Biosensing Techniques
MH  - *Colorimetry
MH  - DNA, Catalytic/chemistry
MH  - G-Quadruplexes
MH  - Humans
MH  - Hydrogen Peroxide/chemistry
MH  - Immunoglobulin E/chemistry/*isolation & purification
MH  - Limit of Detection
MH  - Nucleic Acid Amplification Techniques
MH  - Sulfonic Acids/chemistry
PMC - PMC6567344
OTO - NOTNLM
OT  - DNAzyme
OT  - colorimetric competition detection
OT  - functional nucleic acids
OT  - immunoglobulin E
COIS- The authors declare no conflict of interest.
EDAT- 2019/05/17 06:00
MHDA- 2019/11/14 06:00
PMCR- 2019/05/01
CRDT- 2019/05/17 06:00
PHST- 2019/04/22 00:00 [received]
PHST- 2019/05/10 00:00 [revised]
PHST- 2019/05/11 00:00 [accepted]
PHST- 2019/05/17 06:00 [entrez]
PHST- 2019/05/17 06:00 [pubmed]
PHST- 2019/11/14 06:00 [medline]
PHST- 2019/05/01 00:00 [pmc-release]
AID - s19102224 [pii]
AID - sensors-19-02224 [pii]
AID - 10.3390/s19102224 [doi]
PST - epublish
SO  - Sensors (Basel). 2019 May 14;19(10):2224. doi: 10.3390/s19102224.