PMID- 31092424
OWN - NLM
STAT- MEDLINE
DCOM- 20190523
LR  - 20190523
IS  - 1791-7530 (Electronic)
IS  - 0250-7005 (Linking)
VI  - 39
IP  - 5
DP  - 2019 May
TI  - MCM5 Expression Is Associated With the Grade of Malignancy and Ki-67 Antigen in 
      LSCC.
PG  - 2325-2335
LID - 10.21873/anticanres.13349 [doi]
AB  - BACKGROUND/AIM: The minichromosome maintenance proteins (MCMs) may be potential 
      biomarkers of cancer cell proliferation. They are essential to initiate DNA 
      replication. The aim of the study was to investigate the level of MCM5 expression 
      in benign lesions (BLs) and laryngeal squamous cell cancer (LSCC). MATERIALS AND 
      METHODS: Immunohistochemical (IHC) analysis was carried out on 83 LSCCs and 10 
      BLs. Western-blot, immunofluorescence analysis (IF) and real-time PCR (RT-PCR) 
      were performed using HEp-2 cancer cells and HaCaT keratinocytes. RESULTS: The 
      expression of MCM5 was higher in LSCC than in the BLs (p<0.0001) and was higher 
      in subsequent malignancies of LSCC. Positive correlations were demonstrated 
      between the expression levels of MCM5 and the Ki-67 antigen. In vitro studies 
      have confirmed that the expression of MCM5 is elevated in cancer cells. 
      CONCLUSION: MCM5 protein may be used as a potential marker of cancer cell 
      proliferation in LSCC.
CI  - Copyright(c) 2019, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Nowinska, Katarzyna
AU  - Nowinska K
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland 
      katarzyna.nowinska@umed.wroc.pl.
FAU - Ciesielska, Urszula
AU  - Ciesielska U
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Piotrowska, Aleksandra
AU  - Piotrowska A
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Jablonska, Karolina
AU  - Jablonska K
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Partynska, Aleksandra
AU  - Partynska A
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
FAU - Paprocka, Maria
AU  - Paprocka M
AD  - Laboratory of Cellular Interactions, Ludwik Hirszfeld Institute of Immunology and 
      Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland.
FAU - Zatonski, Tomasz
AU  - Zatonski T
AD  - Department of Otolaryngology, Head & Neck Surgery, Wroclaw Medical University, 
      Wroclaw, Poland.
FAU - Podhorska-Okolow, Marzena
AU  - Podhorska-Okolow M
AD  - Division of Ultrastructure Research, Wroclaw Medical University, Wroclaw, Poland.
FAU - Dziegiel, Piotr
AU  - Dziegiel P
AD  - Department of Human Morphology and Embryology, Division of Histology and 
      Embryology, Wroclaw Medical University, Wroclaw, Poland.
AD  - Department of Physiotherapy, Wroclaw University School of Physical Education, 
      Wroclaw, Poland.
LA  - eng
PT  - Journal Article
DEP - 20190515
PL  - Greece
TA  - Anticancer Res
JT  - Anticancer research
JID - 8102988
RN  - 0 (Biomarkers, Tumor)
RN  - 0 (Cell Cycle Proteins)
RN  - 0 (Ki-67 Antigen)
RN  - 0 (MCM5 protein, human)
SB  - IM
MH  - Aged
MH  - Biomarkers, Tumor/genetics
MH  - Cell Cycle Proteins/*genetics
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Keratinocytes/metabolism
MH  - Ki-67 Antigen/*genetics
MH  - Laryngeal Neoplasms/*genetics/pathology
MH  - Male
MH  - Middle Aged
MH  - Neoplasms/*genetics/pathology
MH  - Squamous Cell Carcinoma of Head and Neck/*genetics/pathology
OTO - NOTNLM
OT  - Ki-67
OT  - MCM
OT  - laryngeal squamous cell cancer
OT  - minichromosome proteins
EDAT- 2019/05/17 06:00
MHDA- 2019/05/24 06:00
CRDT- 2019/05/17 06:00
PHST- 2019/03/20 00:00 [received]
PHST- 2019/04/11 00:00 [revised]
PHST- 2019/04/12 00:00 [accepted]
PHST- 2019/05/17 06:00 [entrez]
PHST- 2019/05/17 06:00 [pubmed]
PHST- 2019/05/24 06:00 [medline]
AID - 39/5/2325 [pii]
AID - 10.21873/anticanres.13349 [doi]
PST - ppublish
SO  - Anticancer Res. 2019 May;39(5):2325-2335. doi: 10.21873/anticanres.13349. Epub 
      2019 May 15.