PMID- 31093645
OWN - NLM
STAT- MEDLINE
DCOM- 20200907
LR  - 20200907
IS  - 1529-7268 (Electronic)
IS  - 0006-3363 (Linking)
VI  - 101
IP  - 1
DP  - 2019 Jul 1
TI  - Novel relationships between porcine fetal size, sex, and endometrial 
      angiogenesisdagger.
PG  - 112-125
LID - 10.1093/biolre/ioz068 [doi]
AB  - It is hypothesized that growth restriction occurs due to inadequate 
      vascularization of the feto-maternal interface. Evidence exists for sexual 
      dimorphism in placental function although associations between fetal sex and the 
      endometrium remain poorly investigated. This study investigated the relationship 
      between porcine fetal size, sex and endometrial angiogenesis at multiple 
      gestational days (GD). Endometrial samples supplying the lightest and closest to 
      mean litter weight (CTMLW), male and female Large White X Landrace conceptuses or 
      fetuses were obtained at GD18, 30, 45, 60, and 90 (n = 5-9 litters/GD). 
      Immunohistochemistry for CD31 revealed a greater number of blood vessels in 
      endometrium supplying females compared to those supplying males at GD45. 
      Endometrial samples supplying the lightest fetuses had fewer blood vessels (GD60) 
      and uterine glands (GD90) compared to those supplying the CTMLW fetuses. 
      Quantitative PCR revealed decreased CD31 (GD60), HPSE and VEGFA (GD90) 
      expression, alongside increased HIF1A (GD45) expression in endometrial samples 
      supplying the lightest compared to the CTMLW fetuses. At GD30, PTGFR, CD31, and 
      VEGFA mRNA expression was increased in samples supplying female fetuses compared 
      to those supplying male fetuses. Intriguingly, decreased expression of ACP5, 
      CD31, HIF1A, and VEGFA mRNAs was observed at GD60 in endometrial samples 
      supplying female fetuses compared to those supplying their male littermates. 
      Endothelial cell branching assays demonstrated impaired endothelial cell 
      branching in response to conditioned media from endometrial samples supplying the 
      lightest and female fetuses compared with the CTMLW and male fetuses, 
      respectively. This study has highlighted that endometrial tissues supplying the 
      lightest and female fetuses have impaired angiogenesis when compared with the 
      CTMLW and female fetuses respectively. Importantly, the relationship between 
      fetal size, sex and endometrial vascularity is dynamic and dependent upon the GD 
      investigated.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of Society 
      for the Study of Reproduction.
FAU - Stenhouse, Claire
AU  - Stenhouse C
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Edinburgh, Midlothian, UK.
FAU - Hogg, Charis O
AU  - Hogg CO
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Edinburgh, Midlothian, UK.
FAU - Ashworth, Cheryl J
AU  - Ashworth CJ
AD  - Developmental Biology Division, The Roslin Institute and Royal (Dick) School of 
      Veterinary Studies, University of Edinburgh, Edinburgh, Midlothian, UK.
LA  - eng
GR  - BB/J004316/1/BB_/Biotechnology and Biological Sciences Research Council/United 
      Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biol Reprod
JT  - Biology of reproduction
JID - 0207224
SB  - IM
MH  - Animals
MH  - Endometrium/*blood supply
MH  - Female
MH  - Fetal Development/physiology
MH  - Fetal Growth Retardation/etiology/veterinary
MH  - Fetal Weight/*physiology
MH  - Fetus/*physiology
MH  - Gene Expression Regulation, Developmental
MH  - Male
MH  - Neovascularization, Physiologic/*physiology
MH  - Placenta/blood supply
MH  - Placentation/physiology
MH  - Pregnancy
MH  - Sex Characteristics
MH  - Swine/*physiology
OTO - NOTNLM
OT  - fetal growth
OT  - intrauterine growth restriction (IUGR)
OT  - porcine
OT  - pregnancy
OT  - reproduction
OT  - sexual dimorphism
OT  - vascularity
EDAT- 2019/05/17 06:00
MHDA- 2020/09/08 06:00
CRDT- 2019/05/17 06:00
PHST- 2018/08/31 00:00 [received]
PHST- 2019/01/17 00:00 [revised]
PHST- 2019/04/22 00:00 [accepted]
PHST- 2019/05/17 06:00 [pubmed]
PHST- 2020/09/08 06:00 [medline]
PHST- 2019/05/17 06:00 [entrez]
AID - 5476496 [pii]
AID - 10.1093/biolre/ioz068 [doi]
PST - ppublish
SO  - Biol Reprod. 2019 Jul 1;101(1):112-125. doi: 10.1093/biolre/ioz068.