PMID- 31096561 OWN - NLM STAT- MEDLINE DCOM- 20191029 LR - 20200225 IS - 1422-0067 (Electronic) IS - 1422-0067 (Linking) VI - 20 IP - 10 DP - 2019 May 15 TI - Cashew Tree Pollen: An Unknown Source of IgE-Reactive Molecules. LID - 10.3390/ijms20102397 [doi] LID - 2397 AB - Pollinosis is sub-diagnosed and rarely studied in tropical countries. Cashew tree pollen has been reported as an allergen source although the knowledge of its immunoglobulin E (IgE)-reactive molecules is lacking. Therefore, this work aimed to identify IgE-reactive molecules and provide a proteomic profile of this pollen. From the 830 proteins identified by shotgun analysis, 163 were annotated to gene ontology, and a list of 39 proteins filtered for high confidence was submitted to the Allfam database where nine were assigned to allergenic families. Thus, 12 patients from the northeast of Brazil with persistent allergic rhinitis and aggravation of symptoms during cashew flowering season were selected. Using a 2D-based approach, we identified 20 IgE-reactive proteins, four already recognized as allergens, including a homolog of the birch isoflavone-reductase (Bet v 6). IgE-reactivity against the extract in native form was confirmed for five patients in ELISA, with three being positive for Bet v 6. Herein, we present a group of patients with rhinitis exposed to cashew tree pollen with the first description of IgE-binding proteins and a proteomic profile of the whole pollen. Cashew tree pollen is considered an important trigger of rhinitis symptoms in clinical practice in the northeast of Brazil, and the elucidation of its allergenic molecules can improve the diagnostics and treatment for allergic patients. FAU - Danella Figo, Daniele AU - Danella Figo D AD - Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. danidanella@hotmail.com. FAU - De Amicis, Karine AU - De Amicis K AD - Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. karineamicis@gmail.com. FAU - Neiva Santos de Aquino, Denise AU - Neiva Santos de Aquino D AD - UNIFOR, Universidade de Fortaleza, Fortaleza 60811-905, Brazil. deniseneiva@gmail.com. FAU - Pomiecinski, Fabiane AU - Pomiecinski F AD - UNIFOR, Universidade de Fortaleza, Fortaleza 60811-905, Brazil. fabiane.alergia@hotmail.com. FAU - Gadermaier, Gabriele AU - Gadermaier G AD - Department of Biosciences, Paris-Lodron-University of Salzburg, 5020 Salzburg, Austria. Gabriele.Gadermaier@sbg.ac.at. FAU - Briza, Peter AU - Briza P AD - Department of Biosciences, Paris-Lodron-University of Salzburg, 5020 Salzburg, Austria. Peter.Briza@sbg.ac.at. FAU - Eduardo Santos Galvao, Clovis AU - Eduardo Santos Galvao C AD - Servico de Imunologia Clinica e Alergia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. clovis.galvao@hc.fm.usp.br. FAU - Bussador do Amaral, Jonatas AU - Bussador do Amaral J AD - ENT Research Lab, Department of Otorhinolaryngology-Head and Neck Surgery, Federal University of Sao Paulo, Sao Paulo 04021-001, Brazil. amaraljb@gmail.com. FAU - de Oliveira Martins, Carlo AU - de Oliveira Martins C AUID- ORCID: 0000-0003-1287-8884 AD - Department of Biological Chemistry, John Innes Centre, Norwich NR4 7UH, UK. carloleia@gmail.com. FAU - Fernandes Morato Castro, Fabio AU - Fernandes Morato Castro F AD - Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. fabio.castro@imabrasil.com.br. AD - Disciplina de Imunologia Clinica e Alergia, Hospital das Clinicas HCFMUSP, Faculdade de Medicina, Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. fabio.castro@imabrasil.com.br. FAU - Kalil, Jorge AU - Kalil J AD - Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. jkalil@usp.br. AD - Laboratorio de Imunologia, Instituto do Coracao, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. jkalil@usp.br. AD - Institute for Investigation in Immunology (iii), INCT, Sao Paulo 01246-903, Brazil. jkalil@usp.br. FAU - Souza Santos, Keity AU - Souza Santos K AUID- ORCID: 0000-0001-5271-4011 AD - Disciplina de Imunologia Clinica e Alergia, Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. keity.santos@fm.usp.br. AD - Laboratorio de Imunologia, Instituto do Coracao, Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo, Sao Paulo 01246-903, Brazil. keity.santos@fm.usp.br. AD - Institute for Investigation in Immunology (iii), INCT, Sao Paulo 01246-903, Brazil. keity.santos@fm.usp.br. LA - eng GR - 2012/14019-2/Fundacao de Amparo a Pesquisa do Estado de Sao Paulo/ GR - PROEX/Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior/ GR - 2016-2018/Fundacao Faculdade de Medicina/ PT - Journal Article DEP - 20190515 PL - Switzerland TA - Int J Mol Sci JT - International journal of molecular sciences JID - 101092791 RN - 0 (Allergens) RN - 0 (Antigens, Plant) RN - 0 (Carrier Proteins) RN - 0 (Plant Proteins) RN - 37341-29-0 (Immunoglobulin E) SB - IM MH - Adolescent MH - Adult MH - Aged MH - Allergens/chemistry/*immunology MH - Anacardium/*chemistry MH - Animals MH - Antigens, Plant/adverse effects/chemistry/genetics/immunology MH - Betula/metabolism MH - Brazil MH - Carrier Proteins/analysis/immunology MH - Child MH - Child, Preschool MH - Cross Reactions/immunology MH - Dermatophagoides farinae MH - Dermatophagoides pteronyssinus MH - Female MH - Humans MH - Immunoglobulin E/*immunology MH - Male MH - Plant Proteins/adverse effects/chemistry/genetics/immunology MH - Pollen/*adverse effects/*chemistry/genetics MH - Proteomics MH - Rhinitis, Allergic, Seasonal/*chemically induced/immunology MH - Skin Tests PMC - PMC6566400 OTO - NOTNLM OT - Brazil OT - aeroallergens OT - novel allergens OT - pollinosis OT - proteome OT - shotgun analysis COIS- The authors declare no conflict of interest. EDAT- 2019/05/18 06:00 MHDA- 2019/10/30 06:00 PMCR- 2019/05/01 CRDT- 2019/05/18 06:00 PHST- 2019/03/21 00:00 [received] PHST- 2019/04/20 00:00 [revised] PHST- 2019/05/02 00:00 [accepted] PHST- 2019/05/18 06:00 [entrez] PHST- 2019/05/18 06:00 [pubmed] PHST- 2019/10/30 06:00 [medline] PHST- 2019/05/01 00:00 [pmc-release] AID - ijms20102397 [pii] AID - ijms-20-02397 [pii] AID - 10.3390/ijms20102397 [doi] PST - epublish SO - Int J Mol Sci. 2019 May 15;20(10):2397. doi: 10.3390/ijms20102397.