PMID- 31097292 OWN - NLM STAT- MEDLINE DCOM- 20200525 LR - 20200525 IS - 2213-2945 (Print) IS - 2213-2953 (Linking) VI - 8 IP - 3 DP - 2019 May-Jun TI - Diagnostic utility of fluorescence in situ hybridization testing on cytology cell blocks for the definitive classification of salivary gland neoplasms. PG - 157-164 LID - S2213-2945(18)30412-5 [pii] LID - 10.1016/j.jasc.2019.01.006 [doi] AB - INTRODUCTION: Fine needle aspiration biopsy (FNAB) is a minimally invasive modality to evaluate salivary gland neoplasms and help guide clinical management. However, significant overlap in the cytomorphology findings among salivary gland neoplasms often renders the definitive diagnosis challenging. Recently, a number of benign and malignant salivary gland tumors have been characterized by specific chromosomal aberrations detectable using fluorescence in situ hybridization (FISH) testing. In the present study, we evaluated the role of FISH testing performed on cytology cell blocks in the diagnosis of salivary gland neoplasms by FNAB. MATERIALS AND METHODS: The data from 57 cases of primary salivary gland tumors diagnosed using FNAB at our institution and sent for ancillary FISH testing between 2012 and 2017 were retrospectively reviewed. The FISH studies were performed on cytology cell blocks, and break-apart probes were used to detect characteristic gene rearrangements for PLAG1, MYB, MAML2, and ETV6 for pleomorphic adenoma, adenoid cystic carcinoma, mucoepidermoid carcinoma, and secretory carcinoma (mammary analogue secretory carcinoma), respectively. Of the 57 cases sent for FISH testing, 6 were excluded because of FISH analysis failure (insufficient cell block cellularity). RESULTS: Of the 51 cases included in the analysis, 15 samples were successfully subclassified after FISH testing, and 10 of these 15 FISH-positive cases were diagnostically confirmed by the surgical pathology review of excision material. Forty cases overall had undergone subsequent excision with the histopathologic follow-up diagnosis available, and all subclassified cases had concordant FNAB, FISH, and excision diagnoses. CONCLUSIONS: FISH testing performed on cytology cell blocks is a useful adjunct in establishing the diagnosis of salivary gland neoplasms by FNAB. CI - Copyright (c) 2019 American Society of Cytopathology. Published by Elsevier Inc. All rights reserved. FAU - Darras, Natasha AU - Darras N AD - Department of Pathology, University of California, San Francisco, San Francisco, California. Electronic address: natasha.darras@ucsf.edu. FAU - Mooney, Kelly L AU - Mooney KL AD - Department of Pathology, Stanford University School of Medicine, Stanford, California. FAU - Long, Steven R AU - Long SR AD - Department of Pathology, University of California, San Francisco, San Francisco, California. LA - eng PT - Evaluation Study PT - Journal Article DEP - 20190125 PL - United States TA - J Am Soc Cytopathol JT - Journal of the American Society of Cytopathology JID - 101613234 SB - IM MH - Adolescent MH - Adult MH - Aged MH - Aged, 80 and over MH - Biopsy, Fine-Needle/standards MH - Carcinoma/classification/genetics/*pathology MH - Child MH - Chromosome Aberrations MH - Female MH - Gene Rearrangement MH - Humans MH - In Situ Hybridization, Fluorescence/methods/*standards MH - Male MH - Middle Aged MH - Salivary Gland Neoplasms/classification/genetics/*pathology OTO - NOTNLM OT - Cytology cell block OT - FISH OT - Fine needle aspiration OT - Fluorescence in situ hybridization OT - Salivary gland EDAT- 2019/05/18 06:00 MHDA- 2020/05/26 06:00 CRDT- 2019/05/18 06:00 PHST- 2018/12/10 00:00 [received] PHST- 2019/01/15 00:00 [revised] PHST- 2019/01/16 00:00 [accepted] PHST- 2019/05/18 06:00 [entrez] PHST- 2019/05/18 06:00 [pubmed] PHST- 2020/05/26 06:00 [medline] AID - S2213-2945(18)30412-5 [pii] AID - 10.1016/j.jasc.2019.01.006 [doi] PST - ppublish SO - J Am Soc Cytopathol. 2019 May-Jun;8(3):157-164. doi: 10.1016/j.jasc.2019.01.006. Epub 2019 Jan 25.