PMID- 31099071
OWN - NLM
STAT- MEDLINE
DCOM- 20200210
LR  - 20200210
IS  - 1365-3024 (Electronic)
IS  - 0141-9838 (Linking)
VI  - 41
IP  - 8
DP  - 2019 Aug
TI  - Trypanosoma brucei gambiense excreted/secreted factors impair 
      lipopolysaccharide-induced maturation and activation of human monocyte-derived 
      dendritic cells.
PG  - e12632
LID - 10.1111/pim.12632 [doi]
AB  - Trypanosoma brucei gambiense, an extracellular eukaryotic flagellate parasite, is 
      the main etiological agent of human African trypanosomiasis (HAT) or sleeping 
      sickness. Dendritic cells (DCs) play a pivotal role at the interface between 
      innate and adaptive immune response and are implicated during HAT. In this study, 
      we investigated the effects of T gambiense and its excreted/secreted factors 
      (ESF) on the phenotype of human monocyte-derived DCs (Mo-DCs). Mo-DCs were 
      cultured with trypanosomes, lipopolysaccharide (LPS), ESF derived from 
      T gambiense bloodstream strain Biyamina (MHOM/SD/82), or both ESF and LPS. 
      Importantly, ESF reduced the expression of the maturation markers HLA-DR and 
      CD83, as well as the secretion of IL-12, TNF-alpha and IL-10, in LPS-stimulated 
      Mo-DCs. During mixed-leucocyte reactions, LPS- plus ESF-exposed DCs induced a 
      non-significant decrease in the IFN-gamma/IL-10 ratio of CD4 + T-cell cytokines. 
      Based on the results presented here, we raise the hypothesis that T gambiense has 
      developed an immune escape strategy through the secretion of paracrine mediators 
      in order to limit maturation and activation of human DCs. The identification of 
      the factor(s) in the T gambiense ESF and of the DCs signalling pathway(s) 
      involved may be important in the development of new therapeutic targets.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Dauchy, Frederic-Antoine
AU  - Dauchy FA
AUID- ORCID: 0000-0002-7727-2851
AD  - Laboratoire de Parasitologie, UMR IRD CIRAD INTERTRYP 177, University of 
      Bordeaux, Bordeaux, France.
AD  - UMR INTERTRYP 177, IRD-CIRAD-University of Bordeaux, Montpellier, France.
AD  - Department of Infectious and Tropical Diseases, Hopital Pellegrin, CHU de 
      Bordeaux, Bordeaux, France.
FAU - Contin-Bordes, Cecile
AU  - Contin-Bordes C
AD  - Laboratoire d'Immunologie et d'Immunogenetique, CHU de Bordeaux, Bordeaux, 
      France.
AD  - UMR 5164 CIRID, University of Bordeaux, Bordeaux, France.
FAU - Nzoumbou-Boko, Romaric
AU  - Nzoumbou-Boko R
AD  - Laboratoire de Parasitologie, UMR IRD CIRAD INTERTRYP 177, University of 
      Bordeaux, Bordeaux, France.
AD  - UMR INTERTRYP 177, IRD-CIRAD-University of Bordeaux, Montpellier, France.
FAU - Bonhivers, Melanie
AU  - Bonhivers M
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, University of Bordeaux, 
      Bordeaux, France.
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, CNRS, Bordeaux, France.
FAU - Landrein, Nicolas
AU  - Landrein N
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, University of Bordeaux, 
      Bordeaux, France.
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, CNRS, Bordeaux, France.
FAU - Robinson, Derrick R
AU  - Robinson DR
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, University of Bordeaux, 
      Bordeaux, France.
AD  - Microbiologie Fondamentale et Pathogenicite, UMR 5234, CNRS, Bordeaux, France.
FAU - Rambert, Jerome
AU  - Rambert J
AD  - Aquiderm, INSERM U 1035, University of Bordeaux, Bordeaux, France.
FAU - Courtois, Pierrette
AU  - Courtois P
AD  - Laboratoire de Parasitologie, UMR IRD CIRAD INTERTRYP 177, University of 
      Bordeaux, Bordeaux, France.
AD  - UMR INTERTRYP 177, IRD-CIRAD-University of Bordeaux, Montpellier, France.
FAU - Daulouede, Sylvie
AU  - Daulouede S
AD  - Laboratoire de Parasitologie, UMR IRD CIRAD INTERTRYP 177, University of 
      Bordeaux, Bordeaux, France.
AD  - UMR INTERTRYP 177, IRD-CIRAD-University of Bordeaux, Montpellier, France.
FAU - Vincendeau, Philippe
AU  - Vincendeau P
AD  - Laboratoire de Parasitologie, UMR IRD CIRAD INTERTRYP 177, University of 
      Bordeaux, Bordeaux, France.
AD  - UMR INTERTRYP 177, IRD-CIRAD-University of Bordeaux, Montpellier, France.
AD  - Laboratoire de Parasitologie, CHU de Bordeaux, Bordeaux, France.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190627
PL  - England
TA  - Parasite Immunol
JT  - Parasite immunology
JID - 7910948
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Protozoan Proteins)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 130068-27-8 (Interleukin-10)
RN  - 187348-17-0 (Interleukin-12)
SB  - IM
MH  - Animals
MH  - Dendritic Cells/*immunology/parasitology
MH  - Female
MH  - HLA-DR Antigens/genetics/immunology
MH  - Host-Parasite Interactions
MH  - Humans
MH  - Interleukin-10/genetics/immunology
MH  - Interleukin-12/genetics/immunology
MH  - Lipopolysaccharides/immunology
MH  - Mice
MH  - Monocytes/*immunology/parasitology
MH  - Protozoan Proteins/genetics/*immunology
MH  - Signal Transduction
MH  - T-Lymphocytes/immunology/parasitology
MH  - Trypanosoma brucei gambiense/genetics/*immunology
MH  - Trypanosomiasis, African/genetics/*immunology/parasitology
MH  - Tumor Necrosis Factor-alpha/genetics/immunology
OTO - NOTNLM
OT  - dendritic cell < cell
OT  - inflammation < disease
OT  - trypanosomiasis < disease
EDAT- 2019/05/18 06:00
MHDA- 2020/02/11 06:00
CRDT- 2019/05/18 06:00
PHST- 2019/03/11 00:00 [received]
PHST- 2019/05/13 00:00 [revised]
PHST- 2019/05/14 00:00 [accepted]
PHST- 2019/05/18 06:00 [pubmed]
PHST- 2020/02/11 06:00 [medline]
PHST- 2019/05/18 06:00 [entrez]
AID - 10.1111/pim.12632 [doi]
PST - ppublish
SO  - Parasite Immunol. 2019 Aug;41(8):e12632. doi: 10.1111/pim.12632. Epub 2019 Jun 
      27.