PMID- 31100040
OWN - NLM
STAT- MEDLINE
DCOM- 20200413
LR  - 20200413
IS  - 1557-8100 (Electronic)
IS  - 1536-2310 (Linking)
VI  - 23
IP  - 5
DP  - 2019 May
TI  - Skin Microbiome Differences in Atopic Dermatitis and Healthy Controls in Egyptian 
      Children and Adults, and Association with Serum Immunoglobulin E.
PG  - 247-260
LID - 10.1089/omi.2019.0011 [doi]
AB  - Atopic dermatitis (AD) is a complex, multifactorial, chronic pruritic 
      inflammatory skin disease. We report the first microbiome study and new insights 
      on the relationship between skin microbiota variation and AD susceptibility in a 
      population sample from Egypt. We characterized the skin microbiome in 75 patients 
      with AD and 20 healthy controls using Illumina MiSeq sequencing of 16S rRNA gene. 
      Overall, bacterial diversity of skin microbiome in patients with AD was less than 
      those of the healthy subjects. Genus level analysis revealed significant 
      abundance variations by age, disease severity, locality, or immune response. 
      Among these genera, Streptococcus, Cutibacterium, and Corynebacterium appeared to 
      be specific signatures for AD in children, adolescents, and adults, respectively, 
      while Staphylococcus was noted as a potential biomarker candidate for AD. 
      Additionally, functional potential of metagenomes shifted the overall metabolic 
      pathways to participate in the exacerbation of disease. Total immunoglobulin E 
      (IgE) levels were positively correlated with relative enrichment of certain 
      Staphylococcus aureus subspecies. Finally, AD-related differences in skin 
      bacterial diversity appeared to be in part linked to the serum IgE level. These 
      new observations attest to the promise of microbiome science and metagenomic 
      analysis in AD specifically, and clinical dermatology broadly.
FAU - Ramadan, Mohammed
AU  - Ramadan M
AD  - 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar 
      University, Assiut, Egypt.
FAU - Solyman, Samar
AU  - Solyman S
AD  - 2 Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal 
      University, Ismaillia, Egypt.
FAU - Yones, Mamdouh
AU  - Yones M
AD  - 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar 
      University, Assiut, Egypt.
FAU - Abdallah, Yasser
AU  - Abdallah Y
AD  - 3 Department of Dermatology, Faculty of Medicine, Al-Azhar University, Assiut, 
      Egypt.
FAU - Halaby, Hamada
AU  - Halaby H
AD  - 1 Department of Microbiology and Immunology, Faculty of Pharmacy, Al-Azhar 
      University, Assiut, Egypt.
FAU - Hanora, Amro
AU  - Hanora A
AD  - 2 Department of Microbiology and Immunology, Faculty of Pharmacy, Suez Canal 
      University, Ismaillia, Egypt.
LA  - eng
PT  - Journal Article
PL  - United States
TA  - OMICS
JT  - Omics : a journal of integrative biology
JID - 101131135
RN  - 0 (RNA, Ribosomal, 16S)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adolescent
MH  - Adult
MH  - Case-Control Studies
MH  - Child
MH  - Child, Preschool
MH  - Corynebacterium/genetics/isolation & purification
MH  - Dermatitis, Atopic/*blood
MH  - Egypt
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*blood
MH  - Male
MH  - RNA, Ribosomal, 16S/genetics
MH  - Skin/*microbiology
MH  - Staphylococcus aureus/genetics/isolation & purification
MH  - Young Adult
OTO - NOTNLM
OT  - Egypt
OT  - atopic dermatitis
OT  - biomarkers
OT  - metagenomics
OT  - microbiome
EDAT- 2019/05/18 06:00
MHDA- 2020/04/14 06:00
CRDT- 2019/05/18 06:00
PHST- 2019/05/18 06:00 [entrez]
PHST- 2019/05/18 06:00 [pubmed]
PHST- 2020/04/14 06:00 [medline]
AID - 10.1089/omi.2019.0011 [doi]
PST - ppublish
SO  - OMICS. 2019 May;23(5):247-260. doi: 10.1089/omi.2019.0011.