PMID- 31100278 OWN - NLM STAT- MEDLINE DCOM- 20190722 LR - 20190722 IS - 1872-7786 (Electronic) IS - 0009-2797 (Linking) VI - 308 DP - 2019 Aug 1 TI - The effect of boletus polysaccharides on diabetic hepatopathy in rats. PG - 61-69 LID - S0009-2797(19)30329-1 [pii] LID - 10.1016/j.cbi.2019.05.013 [doi] AB - The objective of this work was to investigate the effect of boletus polysaccharide (BPS) on diabetic hepatopathy in streptozotocin (STZ)-induced type 2 diabetes mellitus (T2DM) rats for the first time. The rats were fed with high-fat diet (HFD) for 4 weeks and induced with STZ by a single intraperitoneal injection to develop T2DM model. The HFD was given continually for another 4 weeks after diabetes induction, following the drugs of BPS (400 mg/kg bw/day) infused to stomach of rats once a day. After the administration, blood was drawn from the posterior orbital venous plexus of the inner canthus and the rats were then sacrificed. The blood glucose and lipid, alanine transaminase (ALT) and aspartate transaminase (AST) were detected immediately. Besides, their livers were dissected for biochemical and histopathological assays. And the levels of malonaldehyde, glutathione and antioxidant enzymes in liver were detected. In addition, histopathological examinations of liver were performed to verify the liver injury. The expressions of NF-kappaB, TNF-alpha, SREBP1c, and CYP7A1 were test to trace out the mechanistic pathways. Compared with T2DM model group, the blood glucose, TC, TG, ALT, AST, and MDA and so on were significantly reduced, and CAT, SOD, GSH, GPx were significantly increased in the rats treated with BPS. The histopathological examination showed the liver injury in BPS treated rats was alleviated. The expressions of SREBP1c, NF-kappaB and TNF-alpha were significantly decreased, and the expressions of CYP7A1 was significantly increased. All these experimental findings demonstrate that BPS exhibited antidiabetic effects rats possibly through its inhibition of oxidative stress and inflammation, supporting that BPS has a promising therapeutic effect in the treatment of diabetic hepatopathy in rats. CI - Copyright (c) 2019 Elsevier B.V. All rights reserved. FAU - Xiao, Yanhong AU - Xiao Y AD - Department of Biochemistry, Chengde Medical College, Chengde, 067000, Hebei, China. FAU - Chen, Long AU - Chen L AD - Institute of Basic Medical Sciences, Chengde Medical College, Chengde, 067000, Hebei, China. FAU - Fan, Yanfang AU - Fan Y AD - Institute of Basic Medical Sciences, Chengde Medical College, Chengde, 067000, Hebei, China. FAU - Yan, Peng AU - Yan P AD - Anatomical Pathology, Chengde Medical College, Chengde, 067000, Hebei, China. FAU - Li, Suting AU - Li S AD - Department of Biochemistry, Chengde Medical College, Chengde, 067000, Hebei, China. FAU - Zhou, Xiaohui AU - Zhou X AD - Department of Biochemistry, Chengde Medical College, Chengde, 067000, Hebei, China. Electronic address: 250624104@qq.com. LA - eng PT - Journal Article DEP - 20190515 PL - Ireland TA - Chem Biol Interact JT - Chemico-biological interactions JID - 0227276 RN - 0 (Antioxidants) RN - 0 (Blood Glucose) RN - 0 (Hypoglycemic Agents) RN - 0 (NF-kappa B) RN - 0 (Polysaccharides) RN - 0 (Sterol Regulatory Element Binding Protein 2) RN - 0 (Tumor Necrosis Factor-alpha) RN - EC 1.14.14.23 (CYP7A1 protein, rat) RN - EC 1.14.14.23 (Cholesterol 7-alpha-Hydroxylase) RN - EC 2.6.1.1 (Aspartate Aminotransferases) RN - EC 2.6.1.2 (Alanine Transaminase) SB - IM MH - Alanine Transaminase/blood MH - Animals MH - Antioxidants/metabolism MH - Aspartate Aminotransferases/blood MH - Basidiomycota/metabolism MH - Blood Glucose/analysis MH - Cholesterol 7-alpha-Hydroxylase/metabolism MH - Diabetes Mellitus, Experimental/chemically induced/drug therapy/pathology MH - Diet, High-Fat MH - Hypoglycemic Agents/*pharmacology/therapeutic use MH - Liver/drug effects/metabolism/pathology MH - Male MH - NF-kappa B/metabolism MH - Oxidative Stress/*drug effects MH - Polysaccharides/*pharmacology/therapeutic use MH - Rats MH - Rats, Wistar MH - Sterol Regulatory Element Binding Protein 2/metabolism MH - Tumor Necrosis Factor-alpha/metabolism OTO - NOTNLM OT - Boletus polysaccharides OT - Diabetic hepatopathy OT - Liver injury OT - Type 2 diabetic rats EDAT- 2019/05/18 06:00 MHDA- 2019/07/23 06:00 CRDT- 2019/05/18 06:00 PHST- 2019/03/02 00:00 [received] PHST- 2019/04/23 00:00 [revised] PHST- 2019/05/13 00:00 [accepted] PHST- 2019/05/18 06:00 [pubmed] PHST- 2019/07/23 06:00 [medline] PHST- 2019/05/18 06:00 [entrez] AID - S0009-2797(19)30329-1 [pii] AID - 10.1016/j.cbi.2019.05.013 [doi] PST - ppublish SO - Chem Biol Interact. 2019 Aug 1;308:61-69. doi: 10.1016/j.cbi.2019.05.013. Epub 2019 May 15.