PMID- 31100494
OWN - NLM
STAT- MEDLINE
DCOM- 20200624
LR  - 20200624
IS  - 1872-8057 (Electronic)
IS  - 0303-7207 (Linking)
VI  - 498
DP  - 2019 Dec 1
TI  - Estradiol stimulates adipogenesis and Slc2a4/GLUT4 expression via ESR1-mediated 
      activation of CEBPA.
PG  - 110447
LID - S0303-7207(19)30149-2 [pii]
LID - 10.1016/j.mce.2019.05.006 [doi]
AB  - The ability of adipose tissue to expand is dependent on adipocyte differentiation 
      and adipose tissue glucose disposal. The CCAAT/enhancer-binding protein alpha 
      (CEBPA) enhances the expression of the Slc2a4 gene and GLUT4 protein, which are 
      markers of adipocyte differentiation/glucose disposal. We hypothesized estradiol 
      (E2) facilitates adipocyte differentiation/glucose disposal by an estrogen 
      receptor 1 (ESR1)-dependent and CEBPA-mediated mechanism. Our results suggest 
      that E2 (10 nM) has a positive effect on 3T3-L1 adipocyte differentiation (days 
      2-8), lipid accumulation, Slc2a4 and Cebpa mRNA expression, total GLUT4 and 
      nuclear CEBPA contents, and CEBP/Slc2a4-binding activity. Esr1 silencing ( approximately 50%) 
      in mature adipocytes abrogates the 24-h E2 effects on nuclear CEBPA content, 
      Slc2a4/GLUT4 expression and GLUT4 translocation to the cell membrane. Thus, E2 
      stimulates adipocyte differentiation and Slc2a4/GLUT4 expression in an 
      ESR1/CEBPA-mediated pathway. Our data provide mechanistic insight demonstrating 
      E2 participates in adipose-tissue differentiation and glucose transporter 
      expression which ultimately can improve adipose tissue expandability and glycemic 
      control.
CI  - Copyright (c) 2019 Elsevier B.V. All rights reserved.
FAU - Fatima, Luciana A
AU  - Fatima LA
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Campello, Raquel S
AU  - Campello RS
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Barreto-Andrade, Joao N
AU  - Barreto-Andrade JN
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, Sao Paulo, Brazil.
FAU - Passarelli, Marisa
AU  - Passarelli M
AD  - Lipids Laboratory (LIM 10), Medical School, University of Sao Paulo, Sao Paulo, 
      Brazil; Graduate Studies Program in Medicine, University Nove Nove de Julho, Sao 
      Paulo, Brazil.
FAU - Santos, Roberta S
AU  - Santos RS
AD  - Biomedical Research Department, Diabetes and Obesity Research Division, 
      Cedars-Sinai Medical Center, Los Angeles, CA, USA.
FAU - Clegg, Deborah J
AU  - Clegg DJ
AD  - Biomedical Research Department, Diabetes and Obesity Research Division, 
      Cedars-Sinai Medical Center, Los Angeles, CA, USA.
FAU - Machado, Ubiratan F
AU  - Machado UF
AD  - Department of Physiology and Biophysics, Institute of Biomedical Sciences, 
      University of Sao Paulo, Sao Paulo, Brazil. Electronic address: 
      ubiratan@icb.usp.br.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190514
PL  - Ireland
TA  - Mol Cell Endocrinol
JT  - Molecular and cellular endocrinology
JID - 7500844
RN  - 0 (CCAAT-Enhancer-Binding Proteins)
RN  - 0 (CEBPA protein, mouse)
RN  - 0 (Esr1 protein, mouse)
RN  - 0 (Estrogen Receptor alpha)
RN  - 0 (Estrogens)
RN  - 0 (Glucose Transporter Type 4)
RN  - 0 (Slc2a4 protein, mouse)
RN  - 4TI98Z838E (Estradiol)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*cytology/drug effects/metabolism
MH  - Adipogenesis/*drug effects
MH  - Animals
MH  - CCAAT-Enhancer-Binding Proteins/genetics/*metabolism
MH  - Cell Differentiation
MH  - Estradiol/*pharmacology
MH  - Estrogen Receptor alpha/*physiology
MH  - Estrogens/pharmacology
MH  - Female
MH  - Gene Expression Regulation/*drug effects
MH  - Glucose Transporter Type 4/genetics/*metabolism
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Promoter Regions, Genetic
OTO - NOTNLM
OT  - 3T3-L1 adipocytes
OT  - Adipogenesis
OT  - CEBPA
OT  - ESR1-Silencing
OT  - GLUT4
OT  - Slc2a4
EDAT- 2019/05/18 06:00
MHDA- 2020/06/25 06:00
CRDT- 2019/05/18 06:00
PHST- 2019/03/02 00:00 [received]
PHST- 2019/05/10 00:00 [revised]
PHST- 2019/05/10 00:00 [accepted]
PHST- 2019/05/18 06:00 [pubmed]
PHST- 2020/06/25 06:00 [medline]
PHST- 2019/05/18 06:00 [entrez]
AID - S0303-7207(19)30149-2 [pii]
AID - 10.1016/j.mce.2019.05.006 [doi]
PST - ppublish
SO  - Mol Cell Endocrinol. 2019 Dec 1;498:110447. doi: 10.1016/j.mce.2019.05.006. Epub 
      2019 May 14.