PMID- 31100845
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20201001
IS  - 2079-4991 (Print)
IS  - 2079-4991 (Electronic)
IS  - 2079-4991 (Linking)
VI  - 9
IP  - 5
DP  - 2019 May 16
TI  - Detection of Ampicillin-Resistant E. coli Using Novel Nanoprobe-Combined 
      Fluorescence In Situ Hybridization.
LID - 10.3390/nano9050750 [doi]
LID - 750
AB  - Antibiotic-resistant bacteria present a global threat because the infections they 
      cause are difficult to treat. Therefore, it is highly important to develop 
      advanced methods for the identification of antibiotic resistance gene in the 
      virulent bacteria. Here, we report the development of novel nanoprobes for 
      fluorescence in situ hybridization (FISH) and the application of the nanoprobe to 
      the detection of ampicillin-resistant Escherichia coli. The nanoprobe for FISH 
      was synthesized by the modified sol-gel chemistry and the synthesized nanoprobe 
      provided strong fluorescent signals and pH stability even under natural light 
      condition. For the double-identification of bacteria species and 
      ampicillin-resistance with a single probe in situ, the nanoprobes were conjugated 
      to the two kinds of biotinylated probe DNAs; one for E. coli-species specific 
      gene and the other for a drug-resistant gene. By using the nanoprobe-DNA 
      conjugants, we successfully detected the ampicillin-resistant E. coli through the 
      FISH technique. This result suggests the new insight into light stable FISH 
      application of the nanoprobe for a pathogenic antibiotic-resistance bacterium.
FAU - Lee, Wang Sik
AU  - Lee WS
AD  - Environmental Disease Research Center, Korea Research Institute of Bioscience 
      Biotechnology (KRIBB), Daejeon 34141, Korea. wang3026@kribb.re.kr.
AD  - Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, Daejeon 
      34113, Korea. wang3026@kribb.re.kr.
FAU - Lee, Soohyun
AU  - Lee S
AD  - Infectious Disease Research Center, KRIBB, Daejeon 34141, Korea. 
      ysh76@kribb.re.kr.
FAU - Kang, Taejoon
AU  - Kang T
AUID- ORCID: 0000-0002-5387-6458
AD  - Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, Daejeon 
      34113, Korea. kangtaejoon@kribb.re.kr.
AD  - Bionanotechnology Research Center, KRIBB, Daejeon 34141, Korea. 
      kangtaejoon@kribb.re.kr.
FAU - Ryu, Choong-Min
AU  - Ryu CM
AD  - Infectious Disease Research Center, KRIBB, Daejeon 34141, Korea. 
      cmryu@kribb.re.kr.
AD  - Department of Biosystems and Bioengineering, KRIBB School of Biotechnology, UST, 
      Daejeon 34113, Korea. cmryu@kribb.re.kr.
FAU - Jeong, Jinyoung
AU  - Jeong J
AUID- ORCID: 0000-0003-0381-3958
AD  - Environmental Disease Research Center, Korea Research Institute of Bioscience 
      Biotechnology (KRIBB), Daejeon 34141, Korea. jyjeong@kribb.re.kr.
AD  - Department of Nanobiotechnology, KRIBB School of Biotechnology, UST, Daejeon 
      34113, Korea. jyjeong@kribb.re.kr.
LA  - eng
PT  - Journal Article
DEP - 20190516
PL  - Switzerland
TA  - Nanomaterials (Basel)
JT  - Nanomaterials (Basel, Switzerland)
JID - 101610216
PMC - PMC6567190
OTO - NOTNLM
OT  - ampicillin
OT  - antibiotic-resistance
OT  - bacteria
OT  - fluorescence in situ hybridization
OT  - nanoprobe
COIS- The authors declare no conflict of interest.
EDAT- 2019/05/19 06:00
MHDA- 2019/05/19 06:01
PMCR- 2019/05/16
CRDT- 2019/05/19 06:00
PHST- 2019/04/15 00:00 [received]
PHST- 2019/05/04 00:00 [revised]
PHST- 2019/05/12 00:00 [accepted]
PHST- 2019/05/19 06:00 [entrez]
PHST- 2019/05/19 06:00 [pubmed]
PHST- 2019/05/19 06:01 [medline]
PHST- 2019/05/16 00:00 [pmc-release]
AID - nano9050750 [pii]
AID - nanomaterials-09-00750 [pii]
AID - 10.3390/nano9050750 [doi]
PST - epublish
SO  - Nanomaterials (Basel). 2019 May 16;9(5):750. doi: 10.3390/nano9050750.