PMID- 31104196
OWN - NLM
STAT- MEDLINE
DCOM- 20190814
LR  - 20200225
IS  - 1573-7446 (Electronic)
IS  - 0165-7380 (Linking)
VI  - 43
IP  - 3
DP  - 2019 Aug
TI  - Kinetics of the expression of CD163 and CD107a in the lung and tonsil of pigs 
      after infection with PRRSV-1 strains of different virulence.
PG  - 187-195
LID - 10.1007/s11259-019-09755-x [doi]
AB  - The emergence of virulent strains of porcine reproductive and respiratory 
      syndrome virus (PRRSV), causing atypical and severe outbreaks, has been notified 
      worldwide. This study assesses the expression, distribution and kinetics of PRRSV 
      N-protein, CD163 and CD107a in the lung and tonsil from experimentally-infected 
      piglets with three different PRRSV-1 strains: a virulent PRRSV-1 subtype 3 strain 
      (SU1-bel) and two low-virulent subtype 1 strains, Lelystad virus (LV) and 215-06. 
      SU1-bel replicated more efficiently in the lungs and tonsils. The number of 
      CD163(+) cells decreased in both tissues from all infected groups at 7 dpi, 
      followed by an increase at the end of the study, highlighting a negative 
      correlation with the number of N-protein(+)-infected cells. A significant 
      increase in CD107a was observed in all infected groups at 35 dpi but no 
      differences were observed among them. Whereas the initial decrease of CD163(+) 
      cells appears to be associated to virus replication and cell death, the later 
      recovery of the CD163(+) population may be due to either the induction of CD163 
      in immature cells, the recruitment of CD163(+) cells in the area of infection, or 
      both. These results highlight the ability of macrophage subpopulations in 
      infected animals to recover and restore their potential biological functions at 
      one-month post-infection, with the greatest improvement observed in 
      SU1-bel-infected animals.
FAU - Sanchez-Carvajal, Jose M
AU  - Sanchez-Carvajal JM
AUID- ORCID: 0000-0003-0852-3765
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', 14071, 
      Cordoba, Spain. jmsc919@gmail.com.
FAU - Rodriguez-Gomez, Irene M
AU  - Rodriguez-Gomez IM
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', 14071, 
      Cordoba, Spain.
FAU - Carrasco, Librado
AU  - Carrasco L
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', 14071, 
      Cordoba, Spain.
FAU - Barranco, Inmaculada
AU  - Barranco I
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', 14071, 
      Cordoba, Spain.
FAU - Alvarez, Belen
AU  - Alvarez B
AD  - Department of Biotechnology, National Institute for Agricultural and Food 
      Research and Technology (INIA), Madrid, Spain.
FAU - Dominguez, Javier
AU  - Dominguez J
AD  - Department of Biotechnology, National Institute for Agricultural and Food 
      Research and Technology (INIA), Madrid, Spain.
FAU - Salguero, Francisco J
AU  - Salguero FJ
AD  - Department of Pathology, Public Health England, PHE Porton Down, Salisbury, UK.
FAU - Gomez-Laguna, Jaime
AU  - Gomez-Laguna J
AD  - Department of Anatomy and Comparative Pathology, Faculty of Veterinary Medicine, 
      University of Cordoba, International Excellence Agrifood Campus 'ceiA3', 14071, 
      Cordoba, Spain.
LA  - eng
GR  - RYC-2014-16735/Secretaria de Estado de Investigacion, Desarrollo e Innovacion/
GR  - AGL2009-12438/Ministerio de Economia, Industria y Competitividad, Gobierno de 
      Espana/
GR  - AGL2016-76111-R/Ministerio de Economia, Industria y Competitividad, Gobierno de 
      Espana (ES)/
PT  - Journal Article
DEP - 20190518
PL  - Switzerland
TA  - Vet Res Commun
JT  - Veterinary research communications
JID - 8100520
RN  - 0 (Antigens, CD)
RN  - 0 (Antigens, Differentiation, Myelomonocytic)
RN  - 0 (CD163 antigen)
RN  - 0 (Lysosomal-Associated Membrane Protein 1)
RN  - 0 (Nucleocapsid Proteins)
RN  - 0 (Receptors, Cell Surface)
MH  - Animals
MH  - Antigens, CD/*genetics/immunology
MH  - Antigens, Differentiation, Myelomonocytic/*genetics/immunology
MH  - Gene Expression Regulation/*immunology
MH  - Lung/*immunology
MH  - Lysosomal-Associated Membrane Protein 1/*genetics/immunology
MH  - Nucleocapsid Proteins/genetics/metabolism
MH  - Palatine Tonsil/*immunology
MH  - Porcine Reproductive and Respiratory Syndrome/*immunology
MH  - Porcine respiratory and reproductive syndrome virus/*immunology/pathogenicity
MH  - Receptors, Cell Surface/*genetics/immunology
MH  - Swine
MH  - Virulence/immunology
OTO - NOTNLM
OT  - CD107a
OT  - CD163
OT  - Lung
OT  - Macrophages
OT  - PRRSV-1
OT  - Tonsil
EDAT- 2019/05/20 06:00
MHDA- 2019/08/15 06:00
CRDT- 2019/05/20 06:00
PHST- 2019/02/27 00:00 [received]
PHST- 2019/04/30 00:00 [accepted]
PHST- 2019/05/20 06:00 [pubmed]
PHST- 2019/08/15 06:00 [medline]
PHST- 2019/05/20 06:00 [entrez]
AID - 10.1007/s11259-019-09755-x [pii]
AID - 10.1007/s11259-019-09755-x [doi]
PST - ppublish
SO  - Vet Res Commun. 2019 Aug;43(3):187-195. doi: 10.1007/s11259-019-09755-x. Epub 
      2019 May 18.