PMID- 31104439
OWN - NLM
STAT- MEDLINE
DCOM- 20190524
LR  - 20200717
IS  - 0253-2727 (Print)
IS  - 2707-9740 (Electronic)
IS  - 0253-2727 (Linking)
VI  - 40
IP  - 4
DP  - 2019 Apr 14
TI  - [Application of immunophenotypic analysis and molecular genetics in the diagnosis 
      of acute promyelocytic leukemia].
PG  - 288-293
LID - 10.3760/cma.j.issn.0253-2727.2019.04.005 [doi]
AB  - Objective: To investigate the application values of immunophenotypic analysis and 
      molecular genetics in the diagnosis of acute promyelocytic leukemia (APL) . 
      Methods: The retrospective analyses of flow cytometric (FCM) immunophenotypic 
      anyalysis, chromosome karyotype and chromosome fluorescence in situ hybridization 
      (FISH) of 798 outpatient or hospitalization APL patients referred to our hospital 
      between May 2012 and December 2017 were performed to further study the 
      application values of FCM and molecular genetics in the diagnosis of APL. 
      Results: The sensitivity and specificity of FCM were 91.9% and 98.7% 
      respectively. The typical characteristic immunophenotype for APL was as of 
      follows: a high SSC, absence of expression of cluster differntiation (CD) CD34 
      and HLA-DR, and expression or stronger expression of CD33, consistent expression 
      of CD13, CD9, CD123, expression of CD56, CD7, CD2 (sometimes) . The rest 10% of 
      the cases harbored atypical APL phenotypes, generally accompanied by CD34 and/or 
      HLA-DR expression, decreased SSC and often accompanied by CD2 expression, it was 
      difficult to definitively diagnose APL by this FCM phenotype, and their diagnoses 
      depended on the results of genetics or molecular biology tests. Compared with 
      normal individuals, complex karyotypes APL with t (15;17) translocation, other 
      variant translocations and variant t (11;17) , t (5;17) had no significant 
      differences in terms of their FCM phenotypes. Conclusions: FCM could rapidly and 
      effectively diagnose APL. Despite the fact that complex karyotypes with various 
      additional chromosomal abnormalities were detected in approximately one third of 
      APL cases in addition to the pathognomonic t (15;17) (q22;q21) , they had no 
      observable impact on the overall immunophenotype. Molecular and genetic criteria 
      were the golden criteria for the diagnosis of APL. About 10% of immunophenotyping 
      cases relied on molecular genetics for diagnosis.
FAU - Gong, J Y
AU  - Gong JY
AD  - Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical 
      Science and Peking Union Medical College, Tianjin 300020, China.
FAU - Li, Y Y
AU  - Li YY
FAU - Li, C W
AU  - Li CW
FAU - Wang, Y S
AU  - Wang YS
FAU - Liu, Y
AU  - Liu Y
FAU - Wang, C
AU  - Wang C
FAU - Ru, K
AU  - Ru K
FAU - Mi, Y C
AU  - Mi YC
FAU - Wang, J X
AU  - Wang JX
FAU - Wang, H J
AU  - Wang HJ
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhonghua Xue Ye Xue Za Zhi
JT  - Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi
JID - 8212398
SB  - IM
MH  - Flow Cytometry
MH  - Humans
MH  - Immunophenotyping
MH  - In Situ Hybridization, Fluorescence
MH  - *Leukemia, Promyelocytic, Acute/diagnosis
MH  - Retrospective Studies
PMC - PMC7343010
OTO - NOTNLM
OT  - Chromosome karyotype
OT  - Flow cytometry
OT  - Fluorescence in situ hybridization
OT  - Leukemia, promyelocytic, acute
EDAT- 2019/05/21 06:00
MHDA- 2019/05/28 06:00
PMCR- 2019/04/01
CRDT- 2019/05/21 06:00
PHST- 2019/05/21 06:00 [entrez]
PHST- 2019/05/21 06:00 [pubmed]
PHST- 2019/05/28 06:00 [medline]
PHST- 2019/04/01 00:00 [pmc-release]
AID - cjh-40-04-288 [pii]
AID - 10.3760/cma.j.issn.0253-2727.2019.04.005 [doi]
PST - ppublish
SO  - Zhonghua Xue Ye Xue Za Zhi. 2019 Apr 14;40(4):288-293. doi: 
      10.3760/cma.j.issn.0253-2727.2019.04.005.