PMID- 31106369
OWN - NLM
STAT- MEDLINE
DCOM- 20200910
LR  - 20200910
IS  - 1460-2091 (Electronic)
IS  - 0305-7453 (Linking)
VI  - 74
IP  - 8
DP  - 2019 Aug 1
TI  - Characterization of a novel, type II staphylococcal cassette chromosome mec 
      element from an endemic oxacillin-resistant Staphylococcus lugdunensis clone in a 
      hospital setting.
PG  - 2162-2165
LID - 10.1093/jac/dkz189 [doi]
AB  - BACKGROUND: Staphylococcus lugdunensis is a significant pathogen that causes 
      community-acquired and nosocomial infections. The high prevalence of 
      oxacillin-resistant S. lugdunensis (ORSL) is of major concern. Resistance to 
      beta-lactams is caused by acquisition of the staphylococcal cassette chromosome mec 
      (SCCmec) element. The cassette is highly diverse, both structurally and 
      genetically, among CoNS. Isolates carrying SCCmec II-ST6 are the major persistent 
      clones in hospitals. OBJECTIVES: To investigate the structure and evolutionary 
      origin of a novel type II SCCmec element in an endemic ST6 S. lugdunensis clone. 
      METHODS: The structure of the SCCmec II element carried by ST6 strain CGMH-SL118 
      was determined by WGS and compared with those reported previously. RESULTS: A 
      novel 39 kb SCCmec element, SCCmecCGMH-SL118, with a unique mosaic structure 
      comprising 41 ORFs integrated into the 3' end of the rlmH gene, was observed. 
      Some regions of SCCmecCGMH-SL118 were homologous to SCCmec IIa of the prototype 
      MRSA strain N315. The structure of SCCmecCGMH-SL118 was similar to that of SCCmec 
      IIb of the MRSA strain, JCSC3063, mainly lacking the aminoglycoside resistance 
      determinant pUB110 in the J3 region but containing the insertion sequence IS256 
      in the J2 region. Notably, SCCmecCGMH-SL118 deletions in the J1 region compared 
      with SCCmec types IIa and IIb, and a high homology to SCCmec elements of 
      Staphylococcus aureus JCSC4610 and Staphylococcus haemolyticus strain 621 were 
      found. CONCLUSIONS: The genetic diversity of the type II SCCmec element in ORSL 
      suggests that CoNS is a potential reservoir for interspecies transfer of SCCmec 
      to S. aureus in hospitals.
CI  - (c) The Author(s) 2019. Published by Oxford University Press on behalf of the 
      British Society for Antimicrobial Chemotherapy. All rights reserved. For 
      permissions, please email: journals.permissions@oup.com.
FAU - Chang, Shih-Cheng
AU  - Chang SC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, Chang Gung 
      University, Taoyuan, Taiwan.
FAU - Lin, Lee-Chung
AU  - Lin LC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Ge, Mao-Cheng
AU  - Ge MC
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Liu, Tsui-Ping
AU  - Liu TP
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
FAU - Lu, Jang-Jih
AU  - Lu JJ
AD  - Department of Laboratory Medicine, Linkou Chang Gung Memorial Hospital, Taoyuan, 
      Taiwan.
AD  - Department of Medical Biotechnology and Laboratory Science, Chang Gung 
      University, Taoyuan, Taiwan.
AD  - School of Medicine, College of Medicine, Chang Gung University, Taoyuan, Taiwan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Antimicrob Chemother
JT  - The Journal of antimicrobial chemotherapy
JID - 7513617
RN  - 0 (Anti-Bacterial Agents)
RN  - 0 (DNA, Bacterial)
RN  - UH95VD7V76 (Oxacillin)
SB  - IM
MH  - Anti-Bacterial Agents/*pharmacology
MH  - *Chromosomes, Bacterial
MH  - Cross Infection/microbiology
MH  - DNA, Bacterial/genetics
MH  - *Drug Resistance, Bacterial
MH  - Genetic Variation
MH  - Hospitals/statistics & numerical data
MH  - Humans
MH  - Infant, Newborn
MH  - Microbial Sensitivity Tests
MH  - Oxacillin/*pharmacology
MH  - Sequence Analysis, DNA
MH  - Staphylococcal Infections/blood/microbiology
MH  - Staphylococcus lugdunensis/*drug effects/*genetics
MH  - Taiwan
MH  - Whole Genome Sequencing
EDAT- 2019/05/21 06:00
MHDA- 2020/09/12 06:00
CRDT- 2019/05/21 06:00
PHST- 2019/01/05 00:00 [received]
PHST- 2019/03/06 00:00 [revised]
PHST- 2019/04/02 00:00 [accepted]
PHST- 2019/05/21 06:00 [pubmed]
PHST- 2020/09/12 06:00 [medline]
PHST- 2019/05/21 06:00 [entrez]
AID - 5491489 [pii]
AID - 10.1093/jac/dkz189 [doi]
PST - ppublish
SO  - J Antimicrob Chemother. 2019 Aug 1;74(8):2162-2165. doi: 10.1093/jac/dkz189.