PMID- 31106593
OWN - NLM
STAT- MEDLINE
DCOM- 20191104
LR  - 20191104
IS  - 2169-141X (Electronic)
IS  - 2169-1401 (Linking)
VI  - 47
IP  - 1
DP  - 2019 Dec
TI  - Vascular protection of salicin on IL-1beta-induced endothelial inflammatory response 
      and damages in retinal endothelial cells.
PG  - 1995-2002
LID - 10.1080/21691401.2019.1608220 [doi]
AB  - Retinal endothelial cells (RECs) are involved in many ocular diseases such as 
      age-related macular degeneration (AMD) and diabetic retinopathy. Salicin is the 
      major ingredient of willow bark extract, and it has been shown to be a potent 
      anti-inflammatory agent. We aim to explore whether salicin has a vascular 
      protective effect in RECs. Our data indicate that the presence of salicin in RECs 
      culture media ameliorates interleukin-1beta (IL-1beta)-induced cellular reactive oxygen 
      species (ROS) production and NADPH oxidase 4 (NOX-4) expression. At the cellular 
      level, salicin attenuates IL-1beta-induced mitochondrial injury as revealed by its 
      preservation on mitochondrial membrane potential (MMP). Furthermore, salicin 
      inhibits IL-1beta-induced production of pro-inflammatory cytokines such as tumor 
      necrosis factor-alpha (TNF-alpha), interleukin-6 (IL-6), and monocyte chemoattractant 
      protein-1 (MCP-1), vascular adhesion molecules such as intercellular cell 
      adhesion molecule-1 (iCAM-1) and vascular cell adhesion molecule 1 (VCAM-1), and 
      high-mobility group protein 1 (HMGB-1). On the other hand, salicin recovers 
      IL-1beta-induced reduction of endothelial nitric oxide synthase (eNOS) and nitric 
      oxide (NO) release. The presence of salicin significantly reduces the 
      IL-1beta-induced release of lactate dehydrogenase (LDH), indicating that it 
      mitigates cytokine caused cytotoxicity. Mechanistically, we show that salicin 
      suppresses IL-1beta-induced activation of the nuclear factor-kappa B (NF-kappaB) 
      signaling as revealed by its suppression on nuclear p65 protein and transfected 
      NF-kappaB promoter. Collectively, our study demonstrates by multiple facets of its 
      mechanisms that salicin is a protective agent in retinal endothelial cells. These 
      results imply its potential use in therapeutic usage of retinal disease.
FAU - Song, Yu
AU  - Song Y
AD  - a Department of ophthalmology, Weihai Central hospital , Weihai , China.
FAU - Tian, Xia
AU  - Tian X
AD  - a Department of ophthalmology, Weihai Central hospital , Weihai , China.
FAU - Wang, Xuehong
AU  - Wang X
AD  - a Department of ophthalmology, Weihai Central hospital , Weihai , China.
FAU - Feng, Hui
AU  - Feng H
AD  - a Department of ophthalmology, Weihai Central hospital , Weihai , China.
LA  - eng
PT  - Journal Article
PL  - England
TA  - Artif Cells Nanomed Biotechnol
JT  - Artificial cells, nanomedicine, and biotechnology
JID - 101594777
RN  - 0 (Benzyl Alcohols)
RN  - 0 (Glucosides)
RN  - 0 (HMGB1 Protein)
RN  - 0 (Interleukin-1beta)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Vascular Cell Adhesion Molecule-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 4649620TBZ (salicin)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - Benzyl Alcohols/*pharmacology
MH  - Blood Vessels/cytology/*drug effects
MH  - Cell Line
MH  - Endothelial Cells/cytology/*drug effects/metabolism
MH  - Gene Expression Regulation/drug effects
MH  - Glucosides/*pharmacology
MH  - HMGB1 Protein/biosynthesis
MH  - Humans
MH  - Inflammation/chemically induced/pathology
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Interleukin-1beta/*pharmacology
MH  - Intracellular Space/drug effects/metabolism
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Mitochondria/drug effects/metabolism
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Nitric Oxide Synthase Type III/metabolism
MH  - Oxidative Stress/drug effects
MH  - Reactive Oxygen Species/metabolism
MH  - Retina/*cytology
MH  - Vascular Cell Adhesion Molecule-1/metabolism
OTO - NOTNLM
OT  - IL-1beta
OT  - NF-kappaB
OT  - Retinal endothelial cell (RECs)
OT  - inflammation
OT  - reactive oxygen species (ROS)
OT  - salicin
EDAT- 2019/05/21 06:00
MHDA- 2019/11/05 06:00
CRDT- 2019/05/21 06:00
PHST- 2019/05/21 06:00 [entrez]
PHST- 2019/05/21 06:00 [pubmed]
PHST- 2019/11/05 06:00 [medline]
AID - 10.1080/21691401.2019.1608220 [doi]
PST - ppublish
SO  - Artif Cells Nanomed Biotechnol. 2019 Dec;47(1):1995-2002. doi: 
      10.1080/21691401.2019.1608220.