PMID- 31108010
OWN - NLM
STAT- MEDLINE
DCOM- 20200819
LR  - 20200819
IS  - 1365-2222 (Electronic)
IS  - 0954-7894 (Print)
IS  - 0954-7894 (Linking)
VI  - 49
IP  - 8
DP  - 2019 Aug
TI  - Variable IgE cross-reactivity between peanut 2S-albumins: The case for measuring 
      IgE to both Ara h 2 and Ara h 6.
PG  - 1107-1115
LID - 10.1111/cea.13432 [doi]
AB  - BACKGROUND: 2S-albumins Ara h 2 and Ara h 6 are the most potent peanut allergens 
      and levels of specific immunoglobulin E (IgE) towards these proteins are good 
      predictors of clinical reactivity. Because of structural homologies, Ara h 6 is 
      generally considered to cross-react extensively with Ara h 2. OBJECTIVE: We aimed 
      to quantify the IgE cross-reactivity between Ara h 2 and Ara h 6. METHODS: Peanut 
      2S-albumins were purified from raw peanuts. The IgE cross-reactivity between Ara 
      h 2 and Ara h 6 was evaluated with 32 sera from French and US peanut-allergic 
      patients by measuring the residual IgE-binding to one 2S-albumin after depletion 
      of IgE antibodies recognizing the other 2S-albumin. The IgE cross-reactivity 
      between Ara h 2 and Ara h 6 was further investigated by competitive inhibition of 
      IgE-binding and by a model of mast cell degranulation. RESULTS: A highly variable 
      level of IgE cross-reactivity was revealed among the patients. The mean fraction 
      of cross-reactive IgE antibodies represented only 17.1% of 2S-albumins-specific 
      IgE antibodies and was lower than the mean fraction of IgE specific to Ara h 2 
      (57.4%) or to Ara h 6 (25.5%). The higher level of Ara h 2-specific IgE was 
      principally due to the IgE-binding capacity of an insertion containing the 
      repeated immunodominant linear epitope DPYSP(OH) S. The impact of IgE 
      cross-reactivity on diagnostic testing was illustrated with a serum displaying an 
      Ara h 6-specific IgE response of 26 UI/mL that was not associated with the 
      capacity of Ara h 6 to trigger mast cell degranulation. CONCLUSIONS & CLINICAL 
      RELEVANCE: Immunoglobulin E antibodies specific to peanut 2S-albumins are mainly 
      non-cross-reactive, but low-affinity cross-reactivity can affect diagnostic 
      accuracy. Testing IgE-binding to a mixture of 2S-albumins rather than to each 
      separately may enhance diagnostic performance.
CI  - (c) 2019 John Wiley & Sons Ltd.
FAU - Hazebrouck, Stephane
AU  - Hazebrouck S
AUID- ORCID: 0000-0003-4828-7031
AD  - Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Immuno-Allergie 
      Alimentaire, CEA, INRA, Universite Paris-Saclay, Gif-sur-Yvette, France.
FAU - Guillon, Blanche
AU  - Guillon B
AD  - Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Immuno-Allergie 
      Alimentaire, CEA, INRA, Universite Paris-Saclay, Gif-sur-Yvette, France.
FAU - Paty, Evelyne
AU  - Paty E
AD  - Universite Paris Descartes-Assistance Publique des Hopitaux de Paris, Hopital 
      Necker Enfants Malades, Paris, France.
FAU - Dreskin, Stephen C
AU  - Dreskin SC
AD  - Division of Allergy and Clinical Immunology, Department of Medicine, Denver 
      School of Medicine, University of Colorado, Aurora, Colorado.
FAU - Adel-Patient, Karine
AU  - Adel-Patient K
AD  - Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Immuno-Allergie 
      Alimentaire, CEA, INRA, Universite Paris-Saclay, Gif-sur-Yvette, France.
FAU - Bernard, Herve
AU  - Bernard H
AD  - Service de Pharmacologie et Immunoanalyse (SPI), Laboratoire d'Immuno-Allergie 
      Alimentaire, CEA, INRA, Universite Paris-Saclay, Gif-sur-Yvette, France.
LA  - eng
GR  - R01 AI099029/AI/NIAID NIH HHS/United States
GR  - R21 AI135397/AI/NIAID NIH HHS/United States
PT  - Clinical Trial
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20190605
PL  - England
TA  - Clin Exp Allergy
JT  - Clinical and experimental allergy : journal of the British Society for Allergy 
      and Clinical Immunology
JID - 8906443
RN  - 0 (2S Albumins, Plant)
RN  - 0 (Antigens, Plant)
RN  - 0 (Ara h 2 allergen, Arachis hypogaea)
RN  - 0 (Ara h 6 allergen, Arachis hypogaea)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - 2S Albumins, Plant/*immunology
MH  - Adolescent
MH  - Antigens, Plant/*immunology
MH  - Child, Preschool
MH  - Cross Reactions
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood/*immunology
MH  - Peanut Hypersensitivity/blood/*diagnosis/*immunology
PMC - PMC6684383
MID - NIHMS1030881
OTO - NOTNLM
OT  - 2S-albumin
OT  - Ara h 2
OT  - Ara h 6
OT  - IgE
OT  - allergy
OT  - cross-reactivity
OT  - epitope
OT  - peanut
OT  - sensitization
COIS- Disclosure of potential conflict of interest: No conflict of interest in relation 
      to this study.
EDAT- 2019/05/21 06:00
MHDA- 2020/08/20 06:00
PMCR- 2020/08/01
CRDT- 2019/05/21 06:00
PHST- 2018/12/20 00:00 [received]
PHST- 2019/04/15 00:00 [revised]
PHST- 2019/04/25 00:00 [accepted]
PHST- 2019/05/21 06:00 [pubmed]
PHST- 2020/08/20 06:00 [medline]
PHST- 2019/05/21 06:00 [entrez]
PHST- 2020/08/01 00:00 [pmc-release]
AID - 10.1111/cea.13432 [doi]
PST - ppublish
SO  - Clin Exp Allergy. 2019 Aug;49(8):1107-1115. doi: 10.1111/cea.13432. Epub 2019 Jun 
      5.