PMID- 31108107
OWN - NLM
STAT- MEDLINE
DCOM- 20200526
LR  - 20200526
IS  - 1096-0333 (Electronic)
IS  - 0041-008X (Linking)
VI  - 376
DP  - 2019 Aug 1
TI  - Tanshinone prevents alveolar bone loss in ovariectomized osteoporosis rats by 
      up-regulating phosphoglycerate dehydrogenase.
PG  - 9-16
LID - S0041-008X(19)30185-1 [pii]
LID - 10.1016/j.taap.2019.05.014 [doi]
AB  - Osteoporosis is manifested by reduced bone mass. Tanshinone has been shown to 
      affect osteoclast differentiation, but its role in osteoporosis remains less 
      clear. This study aimed to investigate the effects and molecular mechanisms of 
      tanshinone on osteoporosis. Osteoporosis was induced by bilateral ovariectomy 
      (OVX) in adult female rats treated with or without tanshinone. Trabecular bone 
      structure was assessed by micro-computed tomography (micro-CT). Bone marrow 
      stromal cells (BMSCs) were isolated for analysis of stemness and senescence. mRNA 
      levels of age related genes were examined and the role of the gene that was 
      upregulated by tanshinone treatment was suppressed to determine its involvement 
      in tanshinone mediated effects. Finally, the mechanism underlying tanshinone 
      induced gene upregulation was explored. We found that tanshinone treatment 
      restored alveolar bone structure in OVX rats as well as the stemness and 
      senescence status of BMSCs isolated from OVX rats. Tanshinone upregulated Phgdh 
      mRNA levels and inhibition of phosphoglycerate dehydrogenase Phgdh, the protein 
      encoded by the Phgdh gene, abolished the effects of tanshinone on BMSC stemness 
      and senescence. Finally, we found that OVX lead to hypermethylation of the 
      promoter region of Phgdh which was suppressed by tanshinone treatment. Our study 
      shows that tanshinone potently suppress OVX induced osteoporosis and BMSC 
      senescence through upregulation of PHGDH.
CI  - Copyright (c) 2019. Published by Elsevier Inc.
FAU - Wang, Luyao
AU  - Wang L
AD  - The Center of Stomatology, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Cheng, Liangxing
AU  - Cheng L
AD  - Editorial Department of Journal of Basic and Clinical Oncology, the First 
      Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan, China.
FAU - Zhang, Baixia
AU  - Zhang B
AD  - The Center of Stomatology, the First Affiliated Hospital of Zhengzhou University, 
      Zhengzhou 450052, Henan, China.
FAU - Wang, Nan
AU  - Wang N
AD  - Department of Emergency Surgery, the First Affiliated Hospital of Zhengzhou 
      University, Zhengzhou 450052, Henan, China. Electronic address: 
      alpha19820607@163.com.
FAU - Wang, Feng
AU  - Wang F
AD  - Department of Orthopaedic Surgery, Shanghai Sixth People's Hospital East 
      Affiliated to Shanghai University of Medicine & Health Sciences, Shanghai 201306, 
      China. Electronic address: fengwang4@163.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20190517
PL  - United States
TA  - Toxicol Appl Pharmacol
JT  - Toxicology and applied pharmacology
JID - 0416575
RN  - 0 (Abietanes)
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (RNA, Messenger)
RN  - 03UUH3J385 (tanshinone)
RN  - EC 1.1.1.95 (Phosphoglycerate Dehydrogenase)
SB  - IM
MH  - Abietanes/*administration & dosage
MH  - Alveolar Bone Loss/*prevention & control
MH  - Animals
MH  - Cells, Cultured
MH  - Cellular Senescence/drug effects
MH  - DNA Methylation/drug effects
MH  - Drugs, Chinese Herbal
MH  - Female
MH  - Mesenchymal Stem Cells/chemistry/drug effects/physiology
MH  - Osteoporosis/diet therapy/etiology/*physiopathology
MH  - *Ovariectomy
MH  - Phosphoglycerate Dehydrogenase/*genetics
MH  - Promoter Regions, Genetic/drug effects
MH  - RNA, Messenger/analysis
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Up-Regulation/*drug effects
OTO - NOTNLM
OT  - Aging
OT  - Alveolar bone loss
OT  - Osteoporosis
OT  - Phosphoglycerate dehydrogenase (PHGDH)
OT  - Tanshinone
EDAT- 2019/05/21 06:00
MHDA- 2020/05/27 06:00
CRDT- 2019/05/21 06:00
PHST- 2019/05/11 00:00 [received]
PHST- 2019/05/16 00:00 [accepted]
PHST- 2019/05/21 06:00 [pubmed]
PHST- 2020/05/27 06:00 [medline]
PHST- 2019/05/21 06:00 [entrez]
AID - S0041-008X(19)30185-1 [pii]
AID - 10.1016/j.taap.2019.05.014 [doi]
PST - ppublish
SO  - Toxicol Appl Pharmacol. 2019 Aug 1;376:9-16. doi: 10.1016/j.taap.2019.05.014. 
      Epub 2019 May 17.