PMID- 31114309 OWN - NLM STAT- PubMed-not-MEDLINE LR - 20201001 IS - 1178-7090 (Print) IS - 1178-7090 (Electronic) IS - 1178-7090 (Linking) VI - 12 DP - 2019 TI - Decreased expression of lncRNA Malat1 in rat spinal cord contributes to neuropathic pain by increasing neuron excitability after brachial plexus avulsion. PG - 1297-1310 LID - 10.2147/JPR.S195117 [doi] AB - Purpose: Neuropathic pain (NP) is a challenging clinical problem due to its complex pathogenesis. In our previous study using microarray, we found that the levels of lncRNA Malat1 were decreased in the spinal cord of NP rat after brachial plexus avulsion, but its contribution to NP remain unclear. The purpose of this study was to investigate its role in the pathogenesis of NP. Methods: In the NP model of complete brachial plexus avulsion rat, spinal cords were harvested, and fluorescence in situ hybridization (FISH) was used to test the spatial expression of Malat1 and qRT-PCR was used to confirm the quantitative expression of Malat1. In primary cultured neurons, Malat1 expression interfered with adenovirus. Spontaneous electric activities of neurons were tested using multi-electrode arrays and apoptosis of neurons was tested using TUNEL method. The change of intracellular calcium concentration was analyzed using calcium imaging method. Results: Decreased Malat1 expression was confirmed using qRT-PCR, and Malat1 was identified in the cytoplasm of neurons in spinal cord, but not in glia. In vitro, the decrease of Malat1 resulted in an increase in the frequency of spontaneous electric activity in neurons but had no effect on neuronal apoptosis. Further analysis indicated during glutamate stimulation, the change of intracellular calcium concentration in neurons with downregulated Malat1 expression was significantly greater than that in normal neurons. Conclusion: Reduced Malat1 expression may induce NP by increasing neuronal excitability in the spinal cord via regulation of calcium flux. FAU - Meng, Chong AU - Meng C AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Yang, Xun AU - Yang X AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Liu, Yuzhou AU - Liu Y AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Zhou, Yingjie AU - Zhou Y AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Rui, Jing AU - Rui J AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Li, Shenqian AU - Li S AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Xu, Ce AU - Xu C AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Zhuang, Yongqing AU - Zhuang Y AD - Hand Surgery Department, Shenzhen People's Hospital, Shenzhen 518020, People's Republic of China. FAU - Lao, Jie AU - Lao J AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. FAU - Zhao, Xin AU - Zhao X AD - Department of Hand Surgery, Huashan Hospital, Fudan University, Shanghai 200040, People's Republic of China. AD - Key Laboratory of Hand Reconstruction, Ministry of Health, Shanghai 200032, People's Republic of China. AD - Shanghai Key Laboratory of Peripheral Nerve and Microsurgery, Shanghai 200032, People's Republic of China. LA - eng PT - Journal Article DEP - 20190423 PL - New Zealand TA - J Pain Res JT - Journal of pain research JID - 101540514 PMC - PMC6497903 OTO - NOTNLM OT - Malat1 OT - brachial plexus avulsion OT - lncRNA OT - multielectrode array OT - neuron OT - neuropathic pain OT - spinal cord COIS- The authors report no conflicts of interest in this work. EDAT- 2019/05/23 06:00 MHDA- 2019/05/23 06:01 PMCR- 2019/04/23 CRDT- 2019/05/23 06:00 PHST- 2018/11/18 00:00 [received] PHST- 2019/03/15 00:00 [accepted] PHST- 2019/05/23 06:00 [entrez] PHST- 2019/05/23 06:00 [pubmed] PHST- 2019/05/23 06:01 [medline] PHST- 2019/04/23 00:00 [pmc-release] AID - 195117 [pii] AID - 10.2147/JPR.S195117 [doi] PST - epublish SO - J Pain Res. 2019 Apr 23;12:1297-1310. doi: 10.2147/JPR.S195117. eCollection 2019.